Application of Textiles With Plant Textures in Soft Decoration of Home Furnishing

Textiles with plant textures have been widely applied in soft decoration of home furnishing and decoration means are diversified. This paper discusses the design foothold of textiles with plant textures in soft decoration of home furnishing, elaborates their different varieties in soft decoration and the basic principles of application, analyzes the concrete application methods of textiles with plant textures in different soft decoration styles, and predicts their development tendency in soft decoration of home furnishing, thus offering theoretical basis and method reference for the application of textiles with plant textures in soft decoration of modern home furnishing.

Permanence and Stability of an Age-Structured Prey-Predator System with Delays

An age-structured prey-predator model with delays is proposed and analyzed. Mathematical analyses of the model equations with regard to boundedness of solutions, permanence, and stability are analyzed. By using the persistence theory for infinite-dimensional systems, the sufficient conditions for the permanence of the system are obtained. By constructing suitable Lyapunov functions and using an iterative technique, sufficient conditions are also obtained for the global asymptotic stability of the positive equilibrium of the model

Screening and cloning of differentially expressed genes in Dendrobium nobile induced by orchid mycorrhizal fungus promoting the growth

Appropriate mycorrhizal fungi could effectively promote plant growth and development. Our previous research results showed that the growth of Dendrobium nobile was obviously promoted under inoculating one orchid mycorrhizal fungi, Epulorhiza sp. AR-18. To understand the growth-promoting molecular mechanisms, differential displayed real time polymerase chain reaction (DDRT-PCR), reverse Northern blot and Southern blot were used to isolate and identify genes whose transcription were altered in cultured D. nobile plants that were treated with Epulorhiza sp. AR-18. Amplified by 8 primer combinations from one anchor primers and 8 random primers, a total of 14 complementary DNA (cDNA) fragments including 12 differentially expressed cDNA bands were isolated. Reverse northern blot analysis showed that only 2 genes were differentially displayed cDNA bands. One band was an especially expressed fragment, expressed in the treated group but not in the control; while another was a differentially expressed fragment, weak in the treated and strength in the control. Southern blot analysis demonstrated that the two gene fragments were from the plant and not from the fungus. Sequence analysis and database searches revealed no significant homology to any known sequences. The results suggested that the usefulness of messenger RAN (mRNA) differential display technique for the detection of differentially expressed genes in D. nobile whose growth could be promoted by mycorrhizal fungi.Keywords: Dendrobium nobile, differential displayed real time polymerase chain reaction (DDRT-PCR), orchid mycorrhizal fungus, Epulorhiza sp., reverse northern blo

TCMGIS-II based prediction of medicinal plant distribution for conservation planning: a case study of Rheum tanguticum

<p>Abstract</p> <p>Background</p> <p>Many medicinal plants are increasingly endangered due to overexploitation and habitat destruction. To provide reliable references for conservation planning and regional management, this study focuses on large-scale distribution prediction of <it>Rheum tanguticum </it>Maxim. ex Balf (<it>Dahuang</it>).</p> <p>Methods</p> <p>Native habitats were determined by specimen examination. An improved version of GIS-based program for the distribution prediction of traditional Chinese medicine (TCMGIS-II) was employed to integrate national geographic, climate and soil type databases of China. Grid-based distance analysis of climate factors was based on the Mikowski distance and the analysis of soil types was based on grade division. The database of resource survey was employed to assess the reliability of prediction result.</p> <p>Results</p> <p>A total of 660 counties of 17 provinces in China, covering a land area of 3.63 × 10⁶km², shared similar ecological factors with those of native habitats appropriate for <it>R. tanguticum </it>growth.</p> <p>Conclusion</p> <p>TCMGIS-II modeling found the potential habitats of target medicinal plants for their conservation planning. This technology is useful in conservation planning and regional management of medicinal plant resources.</p

Identification of closely related species in Aspergillus through Analysis of Whole-Genome

The challenge of discriminating closely related species persists, notably within clinical diagnostic laboratories for invasive aspergillosis (IA)-related species and food contamination microorganisms with toxin-producing potential. We employed Analysis of the whole-GEnome (AGE) to address the challenges of closely related species within the genus Aspergillus and developed a rapid detection method. First, reliable whole genome data for 77 Aspergillus species were downloaded from the database, and through bioinformatic analysis, specific targets for each species were identified. Subsequently, sequencing was employed to validate these specific targets. Additionally, we developed an on-site detection method targeting a specific target using a genome editing system. Our results indicate that AGE has successfully achieved reliable identification of all IA-related species (Aspergillus fumigatus, Aspergillus niger, Aspergillus nidulans, Aspergillus flavus, and Aspergillus terreus) and three well-known species (A. flavus, Aspergillus parasiticus, and Aspergillus oryzae) within the Aspergillus section. Flavi and AGE have provided species-level-specific targets for 77 species within the genus Aspergillus. Based on these reference targets, the sequencing results targeting specific targets substantiate the efficacy of distinguishing the focal species from its closely related species. Notably, the amalgamation of room-temperature amplification and genome editing techniques demonstrates the capacity for rapid and accurate identification of genomic DNA samples at a concentration as low as 0.1 ng/μl within a concise 30-min timeframe. Importantly, this methodology circumvents the reliance on large specialized instrumentation by presenting a singular tube operational modality and allowing for visualized result assessment. These advancements aptly meet the exigencies of on-site detection requirements for the specified species, facilitating prompt diagnosis and food quality monitoring. Moreover, as an identification method based on species-specific genomic sequences, AGE shows promising potential as an effective tool for epidemiological research and species classification

Crypto-ransomware Detection through Quantitative API-based Behavioral Profiling

With crypto-ransomware's unprecedented scope of impact and evolving level of sophistication, there is an urgent need to pinpoint the security gap and improve the effectiveness of defenses by identifying new detection approaches. Based on our characterization results on dynamic API behaviors of ransomware, we present a new API profiling-based detection mechanism. Our method involves two operations, namely consistency analysis and refinement. We evaluate it against a set of real-world ransomware and also benign samples. We are able to detect all ransomware executions in consistency analysis and reduce the false positive case in refinement. We also conduct in-depth case studies on the most informative API for detection with context

De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis

<p>Abstract</p> <p>Background</p> <p>American ginseng (<it>Panax quinquefolius </it>L.) is one of the most widely used herbal remedies in the world. Its major bioactive constituents are the triterpene saponins known as ginsenosides. However, little is known about ginsenoside biosynthesis in American ginseng, especially the late steps of the pathway.</p> <p>Results</p> <p>In this study, a one-quarter 454 sequencing run produced 209,747 high-quality reads with an average sequence length of 427 bases. <it>De novo </it>assembly generated 31,088 unique sequences containing 16,592 contigs and 14,496 singletons. About 93.1% of the high-quality reads were assembled into contigs with an average 8-fold coverage. A total of 21,684 (69.8%) unique sequences were annotated by a BLAST similarity search against four public sequence databases, and 4,097 of the unique sequences were assigned to specific metabolic pathways by the Kyoto Encyclopedia of Genes and Genomes. Based on the bioinformatic analysis described above, we found all of the known enzymes involved in ginsenoside backbone synthesis, starting from acetyl-CoA via the isoprenoid pathway. Additionally, a total of 150 cytochrome P450 (CYP450) and 235 glycosyltransferase unique sequences were found in the 454 cDNA library, some of which encode enzymes responsible for the conversion of the ginsenoside backbone into the various ginsenosides. Finally, one CYP450 and four UDP-glycosyltransferases were selected as the candidates most likely to be involved in ginsenoside biosynthesis through a methyl jasmonate (MeJA) inducibility experiment and tissue-specific expression pattern analysis based on a real-time PCR assay.</p> <p>Conclusions</p> <p>We demonstrated, with the assistance of the MeJA inducibility experiment and tissue-specific expression pattern analysis, that transcriptome analysis based on 454 pyrosequencing is a powerful tool for determining the genes encoding enzymes responsible for the biosynthesis of secondary metabolites in non-model plants. Additionally, the expressed sequence tags (ESTs) and unique sequences from this study provide an important resource for the scientific community that is interested in the molecular genetics and functional genomics of American ginseng.</p

Association between Ghrelin gene (GHRL) polymorphisms and clinical response to atypical antipsychotic drugs in Han Chinese schizophrenia patients

<p>Abstract</p> <p>Background</p> <p>Ghrelin (<it>GHRL</it>) is a pivotal peptide regulator of food intake, energy balance, and body mass. Weight gain (WG) is a common side effect of the atypical antipsychotics (AAPs) used to treat schizophrenia (SZ). Ghrelin polymorphisms have been associated with pathogenic variations in plasma lipid concentrations, blood pressure, plasma glucose, and body mass index (BMI). However, it is unclear whether <it>GHRL </it>polymorphisms are associated with WG due to AAPs. Furthermore, there is no evidence of an association between <it>GHRL </it>polymorphisms and SZ or the therapeutic response to AAPs. We explored these potential associations by genotyping <it>GHRL </it>alleles in SZ patients and controls. We also examined the relation between these SNPs and changes in metabolic indices during AAP treatment in SZ subgroups distinguished by high or low therapeutic response.</p> <p>Methods</p> <p>Four SNPs (Leu72Met, -501A/C, -604 G/A, and -1062 G > C) were genotyped in 634 schizophrenia patients and 606 control subjects.</p> <p>Results</p> <p>There were no significant differences in allele frequencies, genotype distributions, or the distributions of two SNP haplotypes between SZ patients and healthy controls (<it>P </it>> 0.05). There was also no significant difference in symptom reduction between genotypes after 8 weeks of AAP treatment as measured by positive and negative symptom scale scores (PANSS). However, the -604 G/A polymorphism was associated with a greater BMI increase in response to AAP administration in both APP responders and non-responders as distinguished by PANSS score reduction (<it>P </it>< 0.001). There were also significant differences in WG when the responder group was further subdivided according to the specific AAP prescribed (<it>P </it>< 0.05).</p> <p>Conclusions</p> <p>These four <it>GHRL </it>gene SNPs were not associated with SZ in this Chinese Han population. The -604 G/A polymorphism was associated with significant BW and BMI increases during AAP treatment. Patients exhibiting higher WG showed greater improvements in positive and negative symptoms than patients exhibiting lower weight gain or weight loss.</p