Ordered Mesostructured CdS Nanowire Arrays with Rectifying Properties

Highly ordered mesoporous CdS nanowire arrays were synthesized by using mesoporous silica as hard template and cadmium xanthate (CdR2) as a single precursor. Upon etching silica, mesoporous CdS nanowire arrays were produced with a yield as high as 93 wt%. The nanowire arrays were characterized by XRD, N2adsorption, TEM, and SEM. The results show that the CdS products replicated from the mesoporous silica SBA-15 hard template possess highly ordered hexagonal mesostructure and fiber-like morphology, analogous to the mother template. The current–voltage characteristics of CdS nanoarrays are strongly nonlinear and asymmetrical, showing rectifying diode-like behavior

3D Imaging Algorithm of Directional Borehole Radar Based on Root-MUSIC

A directional borehole radar consists of one transmitting antenna in the borehole and four receiving antennas distributed at equal angles in a ring. The receiving antennas can determine the depth and orientation of targets beside the borehole. However, the problem of target orientation determination and 3D imaging algorithms remains a technological challenge. The MUSIC (multiple signal classification) algorithm requires a peak search, so the accuracy of the operation is limited by the angle interval. Based on the MUSIC algorithm, the Root-MUSIC algorithm is proposed and implemented. By replacing the spectral peak search with calculating the roots of the polynomials greatly improves the orientation recognition accuracy. Finally, the results obtained using the above algorithm are verified with synthetic data and compared with the results of the MUSIC algorithm. The results show that both the MUSIC algorithm and the Root-MUSIC algorithm can achieve very good orientation determination and 3D imaging results. In terms of accuracy, the Root-MUSIC algorithm has an obvious improvement compared with the MUSIC algorithm

Comprehensive Life Cycle Environmental Assessment of Preventive Maintenance Techniques for Asphalt Pavement

Preventive maintenance (PM) is regarded as the most economical maintenance strategy for asphalt pavement, but the life cycle environmental impacts (LCEI) of different PM techniques have not yet been comprehensively assessed and compared, thus hindering sustainable PM planning. This study aims to comprehensively estimate and compared the LCEI of five PM techniques then propose measures to reduce environmental impacts in PM design by using life cycle assessment (LCA), including fog seal with sand, micro-surfacing, composite seal, ultra-thin asphalt overlay, and thin asphalt overlay. Afterwards, ten kinds of LCEI categories and energy consumption of PM techniques were compared from the LCA phases, and inventory inputs perspectives, respectively. Results show that fog seal with sand and micro-surfacing can lower all LCEI scores by more than 50%. The environmental performance of five PM techniques provided by sensitivity analysis indicated that service life may not create significant impact on LCA results to some extent. Moreover, four PM combination plans were developed and compared for environmental performance, and results show that the PM plan only includes seal coat techniques that can reduce the total LCEI by 7–29% in pavement life. Increasing the frequency of seal coat techniques can make the PM plans more sustainable

miR-4780 Derived from N2-Like Neutrophil Exosome Aggravates Epithelial-Mesenchymal Transition and Angiogenesis in Colorectal Cancer

Despite significant advances in diagnostic methods and treatment strategies, the prognosis for patients with advanced colon cancer remains poor, and mortality rates are often high due to metastasis. Increasing evidence showed that it is of significant importance to investigate how the tumor microenvironment participates in the development of colorectal cancer (CRC). In this manuscript, neutrophils were sequentially stimulated with all-trans retinoic acid and transforming growth factor-β in turn to induce the neutrophil polarization. Differentially expressed miRNA in neutrophil exosomes have been sequenced by microarray profile, and the effect of N2-like neutrophil-derived exosomal miR-4780 on epithelial-mesenchymal transition (EMT) and angiogenesis was investigated. In our results, we found that neutrophils were enriched in CRC tumor tissue and that CD11b expression correlated with tumor site and serous membrane invasion. At the same time, we demonstrated that internalization of N2 exosomes exacerbated the viability, migration, and invasion of CRC cell lines and inhibited apoptosis. To further investigate the molecular mechanism, we analyzed the miRNA expression profile in the N2-like neutrophils, which led to the selection of hsa-miR-4780 for the subsequent experiment. The overexpression of miR-4780 from N2-like neutrophil-derived exosomes exacerbated EMT and angiogenesis. Moreover, miR-4780 can regulate its target gene SOX11 to effect EMT and angiogenesis in CRC cell lines. CRC with liver metastasis model also validated that aberrant expression of miR-4780 in N2-like neutrophil exosomes exacerbated tumor metastasis and development of tumor via EMT and angiogenesis. In conclusion, our current findings reveal an important mechanism by which mR-4780 from N2-like neutrophil exosomes exacerbates tumor metastasis and progression via EMT and angiogenesis

Temporal variations in macroinvertebrate communities from the tributaries in the Three Gorges Reservoir Catchment, China

Abstract Background The seasonal variations in macroinvertebrate communities in tropical, temperate and subarctic regions have been observed and well documented to date, but similar studies conducted in subtropical rivers at the regional scale are relatively rare. In this paper, the macroinvertebrate communities from the main tributaries in the Three Gorges Reservoir Catchment (TGRC) were investigated as a function of the four seasons to explore the temporal variations in macroinvertebrate communities and further tests the temporal stability of certain metrics that are based on macroinvertebrates under a routine bioassessment framework. Results The taxa richness reached the highest point in spring, followed by winter, autumn and summer. The taxa Chironomidae, Heptageniidae, Corbiculidae and Baetidae dominated the communities across seasons. The temporal variations in communities were mainly reflected in the changes in taxa proportions between seasons. The percentages of the taxa Heptageniidae and Baetidae were the highest in autumn (normal discharge period) and lowest in summer (high discharge period). The abundance of macroinvertebrates was the lowest in summer, increased in autumn and winter, and then decreased in spring. Natural fluctuations of aquatic ecosystems (temporal effects) resulted in variations that were apparent in macroinvertebrate-based metrics, such as EPT%, Baetidae%, Caenidae%, Ephemerellidae% and Hydropsychidae%. Conclusions The results of our study demonstrated that the macroinvertebrate communities in the main tributaries of the TGRC varied as a function of seasons. This variation was fundamentally similar to the seasonal patterns in subarctic and temperate streams. Different hydro-morphological characteristics and water quality during the high discharge period (summer), low discharge period (winter) and normal discharge period (spring and autumn) strongly affected the distribution patterns of macroinvertebrate communities. Discharge variation among seasons resulted in seasonal fluctuations in the density of macroinvertebrates. In the TGRC, autumn was the important hatching period for mayflies (Ephemeroptera). The variations in metrics related to macroinvertebrates indicated that temporal effects should not be neglected under a biomonitoring framework in future studies

Further confirmation of MBP-induced neurotoxicity

Original data of statistical graph in Figure S

Data from: Myelin basic protein induces neuron-specific toxicity by directly damaging the neuronal plasma membrane

The central nervous system (CNS) insults may cause massive demyelination and lead to the release of myelin-associated proteins including its major component myelin basic protein (MBP). MBP is reported to induce glial activation but its effect on neurons is still little known. Here we found that MBP specifically bound to the extracellular surface of the neuronal plasma membrane and induced neurotoxicity in vitro. This effect of MBP on neurons was basicity-dependent because the binding was blocked by acidic lipids and competed by other basic proteins. Further studies revealed that MBP induced damage to neuronal membrane integrity and function by depolarizing the resting membrane potential, increasing the permeability to cations and other molecules, and decreasing the membrane fluidity. At last, artificial liposome vesicle assay showed that MBP directly disturbed acidic lipid bilayer and resulted in increased membrane permeability. These results revealed that MBP induces neurotoxicity through its direct interaction with acidic components on the extracellular surface of neuronal membrane, which may suggest a possible contribution of MBP to the pathogenesis in the CNS disorders with myelin damage

Data from: Myelin basic protein induces neuron-specific toxicity by directly damaging the neuronal plasma membrane

The central nervous system (CNS) insults may cause massive demyelination and lead to the release of myelin-associated proteins including its major component myelin basic protein (MBP). MBP is reported to induce glial activation but its effect on neurons is still little known. Here we found that MBP specifically bound to the extracellular surface of the neuronal plasma membrane and induced neurotoxicity in vitro. This effect of MBP on neurons was basicity-dependent because the binding was blocked by acidic lipids and competed by other basic proteins. Further studies revealed that MBP induced damage to neuronal membrane integrity and function by depolarizing the resting membrane potential, increasing the permeability to cations and other molecules, and decreasing the membrane fluidity. At last, artificial liposome vesicle assay showed that MBP directly disturbed acidic lipid bilayer and resulted in increased membrane permeability. These results revealed that MBP induces neurotoxicity through its direct interaction with acidic components on the extracellular surface of neuronal membrane, which may suggest a possible contribution of MBP to the pathogenesis in the CNS disorders with myelin damage

<i>Lactiplantibacillus plantarum</i> and <i>Saccharomyces cerevisiae</i>—Fermented Coconut Water Alleviates Dextran Sodium Sulfate-Induced Enteritis in Wenchang Chicken: A Gut Microbiota and Metabolomic Approach

In order to investigate the potential mechanisms of probiotic-fermented coconut water in treating enteritis, this study conducted a comprehensive analysis of the effects of probiotic intervention on the recovery from Dextran Sodium Sulfate-induced acute enteritis in Wenchang chicks. The analysis encompassed the assessment of growth performance, serum indicators, intestinal tissue structure, and metagenomic and metabolomic profiles of cecal contents in 60 Wenchang chicks subjected to intervention. This approach aimed to elucidate the impact of probiotic intervention on the recovery process from acute enteritis at both the genetic and metabolic levels in the avian model. The results revealed that intervention with Saccharomyces cerevisiae Y301 improved the growth rate of chicks. and intervention with Lactiplantibacillus plantarum MS2c regulated the glycerophospholipid metabolism pathway and reshaped the gut microbiota structure in modeling chicks with acute enteritis, reducing the abundance of potentially pathogenic bacteria from the Alistipes and increasing the abundance of potentially beneficial species from the Christensenellaceae. This intervention resulted in the production of specific gut metabolites, including Gentamicin C and polymyxin B2, recognized for their therapeutic effects on acute enteritis. The combined intervention of S. cerevisiae Y301 and L. plantarum MS2c not only enhanced growth performance but also mitigated intestinal wall damage and increased the abundance of gut metabolites such as gentamicin C and polymyxin B2, thereby mitigating symptoms of enteritis. Furthermore, this combined intervention reduced the levels of serum immune markers, including IL-10, IL-6, TNF-α, IFN-γ, and D-lactic acid, thus mitigating intestinal epithelial cell damage and promoting acute enteritis recovery. This study provides crucial insights into the mechanisms of action of probiotics and probiotic-fermented coconut water in acute enteritis recovery, offering new perspectives for sustainable farming practices for Wenchang chicken

Whole-Genome Analysis of <i>Novacetimonas cocois</i> and the Effects of Carbon Sources on Synthesis of Bacterial Cellulose

Novacetimonas cocois WE7 (formally named Komagataeibacter cocois WE7) is a strain isolated from contaminated coconut milk, capable of producing bacterial cellulose (BC). We sequenced its genome to investigate why WE7 cannot synthesize BC from glucose efficiently. It contains about 3.5 Mb and six plasmid DNAs. N. cocois WE7 contains two bcs operons (bacterial cellulose operon, bcs I and bcs II); the absence of bcs III operons may lead to reduced BC production. From genome predictions, glucose, sucrose, fructose, maltose, and glycerol can be utilized to generate BC, with WE7 unable to metabolize carbohydrate carbon sources through the Embden–Meyerhof–Parnas (EMP) pathway, but rather through the Hexose Monophosphate Pathway (HMP) and tricarboxylic acid (TCA) pathways. It has a complete gluconic acid production pathway, suggesting that BC yield might be very low when glucose, maltose, and trehalose are used as carbon sources. This study represents the first genome analysis of N. cocois. This information is crucial for understanding BC production and regulation mechanisms in N. cocois and lays a foundation for constructing engineered strains tailored for diverse BC application purposes