Evaluation of hays produced with Andropogon gayanus grass at different ages using semi - automated in vitro gas production technique

Objetivou-se avaliar o feno da Andropogon gayanus pela técnica in vitro semi-automática de produção de gases. O delineamento estatístico adotado foi o de blocos ao acaso com parcelas subdivididas, sendo as três idades de corte (56, 84 e 112 dias) as parcelas, os inóculos ruminais os blocos, e os tempos de leitura de produção de gases as sub-parcelas. A degradabilidade da matéria seca foi medida nos tempos de 6,12, 24, 48 e 96 horas. As maiores produções (p>0,05) acumuladas de gases foram obtidas com os fenos colhidos aos 56 e 84 dias com 195,1 e 189,4 mL/g de MS respectivamente. Dentre as três idades avaliadas, recomenda-se o feno colhido aos 56 dias de crescimento.In this study were utilized Andropogon gayanus hays produced at different ages of growth (56, 84 and 112 days) for assay of degradability of in vitro dry matter using semi-automated in vitro gas production technique. The statistical design was randomized blocks with split plots. The ages of growth were parcels and degradation time (6, 12, 24, 48 and 96 hours) were subparcels, with five blocks. The observed results indicated that the best hay of A. gayanus are obtained from plants cutted in between 56 days of growth.Facultad de Ciencias Agrarias y Forestale

Evaluation of hays produced with Andropogon gayanus grass at different ages using semi - automated in vitro gas production technique

Objetivou-se avaliar o feno da Andropogon gayanus pela técnica in vitro semi-automática de produção de gases. O delineamento estatístico adotado foi o de blocos ao acaso com parcelas subdivididas, sendo as três idades de corte (56, 84 e 112 dias) as parcelas, os inóculos ruminais os blocos, e os tempos de leitura de produção de gases as sub-parcelas. A degradabilidade da matéria seca foi medida nos tempos de 6,12, 24, 48 e 96 horas. As maiores produções (p>0,05) acumuladas de gases foram obtidas com os fenos colhidos aos 56 e 84 dias com 195,1 e 189,4 mL/g de MS respectivamente. Dentre as três idades avaliadas, recomenda-se o feno colhido aos 56 dias de crescimento.In this study were utilized Andropogon gayanus hays produced at different ages of growth (56, 84 and 112 days) for assay of degradability of in vitro dry matter using semi-automated in vitro gas production technique. The statistical design was randomized blocks with split plots. The ages of growth were parcels and degradation time (6, 12, 24, 48 and 96 hours) were subparcels, with five blocks. The observed results indicated that the best hay of A. gayanus are obtained from plants cutted in between 56 days of growth.Facultad de Ciencias Agrarias y Forestale

Evaluation of hays produced with Andropogon gayanus grass at different ages using semi - automated in vitro gas production technique

Objetivou-se avaliar o feno da Andropogon gayanus pela técnica in vitro semi-automática de produção de gases. O delineamento estatístico adotado foi o de blocos ao acaso com parcelas subdivididas, sendo as três idades de corte (56, 84 e 112 dias) as parcelas, os inóculos ruminais os blocos, e os tempos de leitura de produção de gases as sub-parcelas. A degradabilidade da matéria seca foi medida nos tempos de 6,12, 24, 48 e 96 horas. As maiores produções (p>0,05) acumuladas de gases foram obtidas com os fenos colhidos aos 56 e 84 dias com 195,1 e 189,4 mL/g de MS respectivamente. Dentre as três idades avaliadas, recomenda-se o feno colhido aos 56 dias de crescimento.In this study were utilized Andropogon gayanus hays produced at different ages of growth (56, 84 and 112 days) for assay of degradability of in vitro dry matter using semi-automated in vitro gas production technique. The statistical design was randomized blocks with split plots. The ages of growth were parcels and degradation time (6, 12, 24, 48 and 96 hours) were subparcels, with five blocks. The observed results indicated that the best hay of A. gayanus are obtained from plants cutted in between 56 days of growth.Facultad de Ciencias Agrarias y Forestale

Activity of arginine-phenylalanine and arginine-tryptophan-based surfactants against Staphylococcus aureus

Aims: This study aimed to evaluate the antibacterial effect of two new cationic surfactants based on phenylalanine-arginine (LPAM) and tryptophan-arginine (LTAM). Materials & methods: Antibacterial activity, mechanism of action and interactions with Staphylococcus aureus enzymes were measured through microbiological, flow cytometry and molecular docking assays, respectively. Results & conclusion: These compounds showed antibacterial activity in the range of 4.06-16.24 μg/ml against planktonic cells and no activity against mature biofilms, since they caused a loss of membrane integrity and increased DNA damage, as revealed by flow cytometry analysis. In silico assays revealed the existence of molecular bonds such as hydrogen bonds, mainly with DNA. Therefore, these compounds have promising pharmacological activity against MRSA strains.The authors would like to thank Central Analítica-UFC/CT-INFRA/MCTI- SISANO/Pró-Equipamentos CAPES for the support.Peer reviewe