Identification of specificity H-2.7 as an erythrocyte antigen: Control by an independent locus, H-2G , between the S and D regions

Specificity H-2.7 is expressed predominantly on erythrocytes and controlled by a gene that maps within the H-2 gene complex at a locus, designated as H-2G , which apparently lies between regions S and D . Three phenotypes have been observed with respect to this antigen: a) positive by direct test and absorption (haplotypes H-2 f , H-2 j , H-2 p , H-2 s ); b) positive only by absorption ( H-2 k ); and c) negative ( H-2 b , H-2 d , H-2 q ). New crossover positions have been established for several H-2 recombinants based on classifications for the H-2G locus.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46742/1/251_2005_Article_BF01572281.pd

THE EXPRESSION OF H-2K, H-2D AND Ia ANTIGENS IN VARIOUS TISSUES AS ASSESSED IN Fc RECEPTOR INHIBITION SYSTEMS

The ability of mouse alloantibody to inhibit EA rosette formation and antibody-dependent cell-mediated cytotoxicity (ADCC) was used to study the expression of H-2K, Ia and H-2D antigens in various tissues. As previously reported antisera against each of these groups of antigens inhibited B lymphocyte EA rosette formation. Continuing studies confirmed these observations but established that quantitative differences may exist in the ease with which antibody against antigens in each region can inhibit EA rosettes: anti H-2D and anti-Ia seemed stronger relative to their cytotoxic titres than anti H-2K. Possible reasons for this are discussed. When rosette forming cells from other tissues were studied, (bone marrow cells, peritoneal macrophages and tumour cells), they were inhibited by anti H-2K and anti H-2D sera but not by anti Ia sera, presumably reflecting the restricted distribution of Ia antigens in those tissues. Inhibition of ADCC by various antisera reflected qualitatively and quantitatively the expression of H-2 antigens in various tissues: whereas effector cell activity in spleen, bone marrow, or peritoneal cell populations was inhibited by anti H-2 or anti-Ia sera, the amount of inhibition observed with anti-Ia was much less when the tissue expressed little Ia antigen (bone marrow) than when it expressed abundant Ia antigen (spleen). The ability of cytotoxicity inhibition to detect antibody coated cells was used to assess the relative amount of Ia antigen on thymus and on lymph node cells, showing significant amounts of Ia antigen on thymus cells. Fc receptor inhibition studies may thus be useful as new approaches to the study of the expression of the antigens of the major histocompatibility complex.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74647/1/j.1744-313X.1975.tb00547.x.pd

Tissue graft rejection in mice

A liver-slice to kidney-bed grafting system was used to study the course of rejection of a specific tissue across various genetic barriers in inbred strains of mice. Rejection or survival, scored histologically at various times after grafting, demonstrated that multiple non H-2 differences cause rejection at least as rapidly as H-2 differences. Differences at the K end of the mouse major histocompatibility complex cause tissue rejection more rapidly than do differences at the D end of the complex. The latter differences cause chronic rejection similar to that found across several minor H locus barriers. The H-2 haplotype carried by the recipient or the strength of the H-2 antigens of the donor affect the survival time in liver tissue grafts. Studies employing this model system will contribute to the definition of different immunogenetic parameters affecting survival of various tissues in a genetically well-defined animal model.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46746/1/251_2005_Article_BF01576941.pd

A serum protein polymorphism determinant on chromosome 9 of Mus musculus.

Genetic polymorphism for a previously undescribed serum protein has been found among inbred strains of Mus musculus. The new serum protein locus, gene symbol Sep-1, has been located on Chromosome 9, gene order Lap-1--Sep-1--Mpi-1--d--Mod-1, by utilizing information obtained from 52 recombinant inbred strains together with standard genetic backcrosses. The strain distribution pattern for this locus, supernatant malic enzyme, and transferrin also on Chromosome 9, are given for 67 inbred strains. Because the genotype of SEP-1 can be determined for individual mice without killing them, Sep-1 is a very useful gene in linkage studies and experimental biology