A frailty census of older adults in the emergency department and acute inpatient settings of a model 4 hospital in the Mid‑West of Ireland

Background Frailty is a risk factor for presentation to the ED, in-hospital mortality, prolonged hospital stays and functional decline at discharge. Profiling the prevalence and level of frailty within the acute hospital setting is vital to ensure evidence-based practice and service development within the construct of frailty. The aim of this cross-sectional study was to establish the prevalence of frailty and co-morbidities among older adults in an acute hospital setting. Methods Data collection was undertaken by clinical research nurses and advanced nurse practitioners experienced in assessing older adults. All patients aged≥65 years and admitted to a medical or surgical inpatient setting between 08:00 and 20:00 and who attended the ED over a 24-h period were screened using validated frailty and co-morbidity scales. Age and gender demographics, Clinical Frailty Scale (CFS), Charlson Co-morbidity Index (CCI) and admitting specialty (medical/ surgical) were collected. Descriptive statistics were used to profile the cohort, and p values were calculated to ascertain the significance of results. Results Within a sample of 413 inpatients, 291 (70%) were≥65 years and therefore were included in the study. 202 of these 291 older adults (70%) were≥75 years. Frailty was investigated using validated clinical cut-offs on the CFS (not frail<5; frail≥5). Comorbidities were investigated using the Charlson Comorbidity Index (mild 1–2; moderate 3–4; severe≥5). The median CFS was 6 indicating moderate frailty levels, and the median CCI score was 3 denoting moderate co-morbidity. In the inpatient cohort, 245 (84%) screened positive for frailty, while 223 (75%) had moderate-severe co-morbidity (CCI Mod 3–4, severe≥5). No significant differences were observed across genders for CFS and CCI. In the ED, 81 patients who attended the ED were≥65 years. The median CFS was 6 (moderate frailty), and the median CCI was 5 (severe co-morbidity level). Seventy-four percent (60) of participants screened positively for frailty (CFS≥5), and 31% (25) had a CFS of 7 or greater (severely frail). Ninety-six percent (78) of patients had a moderate-severe level of comorbidity. No significant associations were found between the CFS and CCI and ED participants age, gender, and medical/surgical speciality usage. Conclusion There is a high prevalence of frailty and co-morbidity among older adults who present to the ED and require inpatient care. This may contribute to increased waiting times, lengths of stay, and the need for specialist intervention. With an increased focus on the integration of care for older adults across care transitions, there is a clear need for expansion of frailty-based services, staff training in frailty care and multidisciplinary team resources across the hospital and community setting</p

HeLa FUCCI ANALYSIS

Analysis of cell cycle transition from G1 to S using Fucci vectors in HeLa cells as well as analysis of mean Mitosis length in the cell typ

RelA-DsRedxp E2F1-Venus BAC stables FCS Autocorrolation and cross-corrolation data

RelA-DsRedxp E2F1-Venus BAC stables FCS autocorrolation and cross-corrolation dat

Peak RelA Nuclear Amplitude for HeLa Cells

Peak RelA Nuclear Amplitude for HeLa Cells following TNF stimulation arranged by virtual synchronization of cell cycle. Single cell traces upon which the peak amplitude data is based are also include

RelA-DsRedxp E2F1-Venus BAC stables FCS molecular fluoresence Data

Molecular fluorescence counts in single cells of RelA-DsRedxp E2F1-Venus BAC stables and correlation data

Effect of TNFalpha on HeLa and SK-N-AS cell cycle length

Cell cycle lengths for SK-N-AS and HeLa cells without and with TNFalpha stimulation. Effect extension on cell cycle following stimulation is also shown at G1 and S phases

BAC BAC Stable cell phase Meta data_Dryad

Traces of BAC-BAC E2F-1-Venus and Rel-A-dsRedexpress sorted into different cell cycle phases

Psychotherapeutic interventions for burns patients and the potential use with Stevens-Johnson syndrome and toxic epidermal necrolysis patients: A systematic integrative review

Background The existing evidence demonstrates that survivors of SJS/TEN have reported long-lasting psychological effects of their condition. Burns patients experience similar psychological effects. It is important to look at ways to help allay the psychological complications of SJS/ TEN. As there is an absence of evidence on SJS/TEN psychotherapeutic interventions, it was judged to be beneficial to determine the evidence underpinning psychotherapeutic interventions used with burns patients.  Aims and objectives The aim of this systematic integrative review was to synthesize the evidence relating to psychotherapeutic interventions used with adult burns patients and patients with SJS/TEN.  Method The systematic review was guided by Whittemore and Knafl’s integrative review process and the PRISMA guidelines. Nine databases were searched for English and French language papers published January 2008 to January 2021. The protocol for the review was registered with PROSPERO.  Conclusion Following further research, some of the interventions deployed in burns patients may be applicable to SJS/TEN patients, particularly stress reduction techniques. In addition, the caring behaviours such as compassion, respect, and getting to know the patient as a person are important components to psychological care.  </p

120210 AS FUCCI O+G CTRL ANALYSIS

Analysis of cell cycle transition from G1 to S using Fucci vectors in SK-N-AS cells as well as analysis of mean Mitosis length in the cell typ

The quality-of-life impact of Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) on patients' lives: An interpretative descriptive qualitative study

Background: Much has been documented about the physical sequelae of Stevens Johnson Syndrome (SJS) and Toxic Epidermal Necrolysis (TEN). Whilst less documented, it is recognised that patients can have long lasting psychological sequelae. There is a lack of qualitative research on the quality of life (QoL) experiences of adults who have been diagnosed with SJS/TEN.Objectives: To explore the experiences of adults who had SJS/TEN and how these experiences relate to their QoL.Methods: Using an interpretative descriptive qualitative study, a purposive sample of 18 adults with SJS/TEN were interviewed using in depth semi structured interviews. Data were analysed using content analysis.Results: Two themes were constructed, each with two categories. Theme 1, Psychosocial Impacts included the categories 'The Self and Others', and 'A Changed Perspective on Life'. Theme 2, The Chronicity of Sequelae comprised the categories 'Multi Organ Involvement', and 'Further Consequences of TEN'.Conclusions: Findings highlighted that SJS/TEN had a significant impact on the different quality-of-life experiences of participants including psychological, physical, social, educational and occupational. Many expressed challenges they faced following discharge from hospital, including gaps in psychological care, navigating disjointed care pathways and lack of coordinated care. If SJS/TEN is viewed as a chronic condition, it is important that researchers and clinicians study the long-term effects of SJS/TEN on people's lives to aid in developing a plan of care to enhance the QoL for this cohort. Psychological and quality of life assessments following discharge from hospital require consideration.</p