Karyomorphology of skin diseases in human population

The aim of the present study is to determine the incidence of chromosomal abnormalities in skin diseases. Patients were analyzed for chromosomal aberrations using peripheral lymphocyte culture. Cytogenetic analysis was carried out in 27 people affected by Vitiligo, Psoriasis, Parthenia. Lymphocytes culture was done and subjected to colcemid, hypotonic treatment, fixation and air drying.  GTG banded chromosomes were identified and counted for abnormalities. One way ANOVA was used to analyze the collected data. Tukey-Kramer Multiple Comparisons Test was used to determine differences between the means. Comparing the groups on the basis of type of chromosomal abnormalities, the differences between the means was found to be non significant (P>0.05) in all the comparisons. However, it was found to be significant for acrocentric association in Vitiligo subjects as compared to the controls. The means for premature centromeric divisions (Parthenia Vs Vitiligo) were also found to be significantly different

Plasma Electrophoresis and Phagocytic Index Screening of Some Indigenous Vegetables Subjected to Preclinical Models

50 % of methanolic extract Colocasia esculenta, Moringa oliefera, Luffa cylindrica, and Hibiscus esculentus were subjected to immunomodulatory activity in Swiss albino mice either sex. Mice were treated with five days of dosing of Colocasia esculenta 50 mg/kg bw, Colocasia esculenta 100 mg/kg bw, Moringa oliefera 200mg/kg bw, Moringa oleifera 400 mg/kg bw, Luffa cylindrica 100 mg/kg bw, Luffa cylindrica 200 mg/kg bw, Hibiscus esculentus 100 mg/kg bw, and Hibiscus esculentus 200 mg/kg bw. Cyclosporine (2.5 mg/kg) used as a standard reference drug for 5 days. Investigation of immunomodulator activity of these 50 % of methanolic extract of drugs to parentage of yeast digestion form 24 hours the peritoneal fluid culture and electrophoretic plasma protein band albumin, alpha 1, alpha 2, beta and gamma respectively from blood plasma were observed using parameters phagocytosis and plasma electrophoresis. Also investigated the ulcerogenic effect or any toxic effect of plant extract by histopathology study of crypt, villi and goblet cells with reference to standard drug cyclosporine. As regards these parameters, Hibiscus esculentus 100 and 200 mg/ kg bw dose, Moringa oleifera 200 and 400 mg/ kg bw dose and Luffa cylindrica 200mg/kg bw elicited a moderately significant increase in the % of yeast digestion (P < 0.001) respectively and Luffa cylindrica 100mg/kg bw significant increase in the % of yeast digestion (P < 0.01). Hibiscus esculentus showed significant dose dependent increase and Moringa oleifera decrease phagocytic activity of macrophages. Hibiscus esculentus 200 mg/ kg bw dose and Moringa oleifera 200 significantly increased (P < 0.01) the Gamma globulin.However, our present study revealed and signatured for their immunomodulator enhancing property. As in Asian subcontinent daily there vegetables are cooked and served with know and unknown of its potential function against different diseases. If there vegetables properly ruled out for their pharmacological aspect then it may add diamond in the crown of dietician which has been bother every day today life but over looked exponetentially.Keywords:- Colocasia esculenta, Moringa oliefera, Luffa cylindrica, and Hibiscus esculentu

Expanding the repertoire of pyrrolidyl PNA analogues for DNA/RNA hybridization selectivity: aminoethylpyrrolidinone PNA (aepone-PNA)

New PNA analogues derived from aminoethylpyrrolidin- 5-one backbone show stabilization of aepone-PNA:DNA hybrids and destabilization of the corresponding RNA hybrids compared to unmodified PNA

PNA C-C<SUP>+</SUP> i-motif: superior stability of PNA TC<SUB>8</SUB> tetraplexes compared to DNA TC<SUB>8</SUB> tetraplexes at low pH

Study of self-assembly of PNA TC8 monitored by UV thermal transition at 295 nm indicates formation of a C-C+ tetraplex (i-motif) in acidic pH, with higher stability than the analogous dTC8

Radiation Protective Potentiality of Adhatoda vasica

The present study forecasts the extensive damage caused by radiotherapy and chemotherapy treatment for cancer in the genetic setup of an individual. To counteract this damage, radioprotective potentiality of 50% Methanolic extract of Adhatoda vasica was evaluated through Cyto-Geno analysis.50% Methanolic extract of Adhatoda vasica leaves was used as a drug whose radioprotective potentiality was to be investigated. Human peripheral lymphocytes were cultured using RPMI-1640 media, harvested and observed under microscope for chromosomal aberration assay.Total 200 metaphases were counted for each group. All the metaphases were found to be normal in Control group. The frequency of normal metaphases greatly increased after pretreatment with both low and high drug dose (50mg/Kg body weight and 100mg/Kg body weight) concentration. The average normal metaphase in Radiation Control was 118 out of 200 metaphases but after pretreatment with 50% Methanolic extract of Adhatoda vasica the number of normal metaphase increased to 179 at 50mg/Kg body weight and 184 at 100mg/Kg body weight. It was concluded that 50% Methanolic Extract of Adhatoda vasica with both low and high drug doses have shown its potentiality as a radio protector against the therapeutically induced mutations which can prove to be a contributor in cancer management in future. Such indigenous Indian, herbal, cost effective, poor man friendly drug will definitely be a potential adjuvant to cancer treatments like radiotherapy and chemotherapy since Amifostine a well known radioprotector given to the patients at the time of cancer therapy is expensive and has its own side effects.Keywords: Radiation Protective, Adhatoda vasica, metaphase

Radiation Protective Potentiality of Adhatoda vasica

The present study forecasts the extensive damage caused by radiotherapy and chemotherapy treatment for cancer in the genetic setup of an individual. To counteract this damage, radioprotective potentiality of 50% Methanolic extract of Adhatoda vasica was evaluated through Cyto-Geno analysis.50% Methanolic extract of Adhatoda vasica leaves was used as a drug whose radioprotective potentiality was to be investigated. Human peripheral lymphocytes were cultured using RPMI-1640 media, harvested and observed under microscope for chromosomal aberration assay.Total 200 metaphases were counted for each group. All the metaphases were found to be normal in Control group. The frequency of normal metaphases greatly increased after pretreatment with both low and high drug dose (50mg/Kg body weight and 100mg/Kg body weight) concentration. The average normal metaphase in Radiation Control was 118 out of 200 metaphases but after pretreatment with 50% Methanolic extract of Adhatoda vasica the number of normal metaphase increased to 179 at 50mg/Kg body weight and 184 at 100mg/Kg body weight. It was concluded that 50% Methanolic Extract of Adhatoda vasica with both low and high drug doses have shown its potentiality as a radio protector against the therapeutically induced mutations which can prove to be a contributor in cancer management in future. Such indigenous Indian, herbal, cost effective, poor man friendly drug will definitely be a potential adjuvant to cancer treatments like radiotherapy and chemotherapy since Amifostine a well known radioprotector given to the patients at the time of cancer therapy is expensive and has its own side effects.Keywords: Radiation Protective, Adhatoda vasica, metaphase

Integrating IT-Enabled Social Networks with Transaction Cost Economics and the Resource Based View of the Firm

Prior research has mostly focused on transaction cost economics (TCE) to interpret the effect of information systems (IS) on organizational governance structures. A TCE based approach predicts that information technology (IT) will lead to increased use of electronic markets to coordinate economic transactions from electronic hierarchies. However, there is contradictory evidence in the literature regarding the rise and importance of cooperative relationships, joint ventures, and value-added partnerships integrated through information systems. To reconcile these contradictions, this paper analyzes the effect of IT on governance structures based on the TCE, social network theory, and the resource based view (RBV) of the firm. The most important aspect of this paper is that instead of overemphasizing the economic perspective, as has been done in prior IS research, it pays equal attention to economic, social, and knowledge perspectives of the firm. By considering variables such as product demand uncertainty, human specificity, task complexity, and frequency of interaction, the effect of IT on governance structure has been analyzed. In this paper, we suggest that, in knowledge intensive companies, a greater degree of outsourcing will take place, not through markets as hypothesized by earlier researchers, but through an increasing number of social networks. This differentiation can not be understood in simple economic terms because social networks are not based on contracts. Therefore, we suggest that the integration of IT-enabled social networks with the TCE and RBV of the firm leads to a better understanding and improvement of decision-making and corporate governance structures in knowledge intensive firms

COMPARATIVE IN VITRO ANTIOXIDANT ACTIVITY OF ETHYL ACETATE AND ETHANOL EXTRACTS OF CALLICARPA MACROPHYLLA

Objective: The objective of the study was to evaluate and compare the antioxidant activity of (EA and E) ethyl acetate and ethanol extracts of (CM) Callicarpa macrophylla. Methods: The physiochemical parameters were assessed according to guidelines given by the world health organization. The total content of phenols and flavonoids was assessed by Folin–Ciocalteu and aluminum chloride methods. In vitro, antioxidant activity was screened by (DPPH) 1, 1-diphenyl-2- picrylhydrazyl and(H2O2) hydrogen peroxide scavenging and reducing power assay. Results: The physicochemical parameters fulfilled the standards of WHO guidelines. Total phenol and flavonoid content were more in ethanol extract as compared to ethyl acetate extract of CM. The antioxidant activity of ethanol extract was further high as compared to ethyl acetate extract of Callicarpa macrophylla. The IC50 of Callicarpa macrophylla ethanol extract was less than the ethyl acetate extract. So, more antioxidant activity of ethanol extract compared to ethyl acetate extract of CM. Conclusion: Overall, both the extracts showed antioxidant activity and can be used further for diseases that can be managed using antioxidants. Ethanol extract possessed significant antioxidant effects than the ethyl acetate extract

A REVIEW FROM HISTORICAL TO CURRENT-CELASTRUS PANICULATUS

Celastrus paniculatus is commonly known as “Malkangani”, widely distributed in the Maldives, Australia, China, Cambodia, Malaysia, Taiwan, Nepal, Thailand as well as in the Pacific Islands and all over India mainly Maharashtra, Orissa and Andaman and Nicobar group of Islands on an altitude of 1800m. It climbs up to over 10m. The leaves are ovate or elliptic in shape with dentate margin. Seeds are ellipsoid or ovoid, yellowish-brown in color and grow inside the capsules. Celastrus paniculatus (Malkangni) is used in Ayurveda as a nervine tonic, tranquilizer and diuretic and in rheumatism, gout, leprosy and asthma. Different Parts of Celastrus paniculatus after extraction and fractionation give different active constituents such as sesquiterpene esters-malkanguinol, malkangunin, sesquiterpene alkaloids-celapanin, celapanigin, alkaloids-celastrine, paniculatine, fatty acids-oleic acid, palmittic acid, linoleic acid and stearic acid, crystalline substance tetracasanol and sterol. Different pharmacological activities are anti-rheumatic, anti-fungal, nootropic activity, antimalarial activity, anti spermatogenic effect, anti-anxiety and anti-atherosclerotic effect. In the present review, our target is to search, bring together and compile the data of Celastrus paniculatus, which have less side effects and very valuable for the treatment of rheumatism. Related information is procured from various scientific publications using online, seek out engines such as Google scholar, Pubmed and Science Direct. A total of 200 articles was reviewed out of which 55 articles are selected to review for the description of the plant, parts used, chemical constituents, traditional uses and for reported activities