The role of religiousness on substance-use disorder treatment outcomes: a comparison of black and white adolescents

This study compares 41 Black and 124 White adolescents at intake and discharge from a residential treatment program for substance-use disorders. Study data were obtained as part of a larger study (N = 195) that sought to assess the relationship of helping behavior and addiction recovery. This post-hoc analysis aims to identify cultural strengths that may be associated with recovery from substance-use disorders among Black adolescents. Using regression analyses and controlling for the severity of substance use and background variables that distinguish racial groups, religious practices and behaviors at intake were examined. Specifically, Black youth and White youth were compared on treatment outcomes, including alcohol or drug use during treatment, drug craving, 12-Step work, and 12-Step helping. The burden of health and socioeconomic disparities at intake did not disproportionately disfavor Black adolescents. Outcomes related to 12-Step measures were similar between Black and White youth. White adolescents reported higher craving scores at discharge, and Black adolescents were more likely to use drugs during treatment. High levels of religiousness at treatment intake were linked to greater 12-Step work and greater 12-Step helping at discharge. High levels of religiousness at intake were not related to drug use during treatment or to craving scores at discharge. The relationship between intake levels of religiousness and treatment-related outcomes did not differ by race.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437261/Accepted manuscrip

Effects of Lime Amendment on Antibiotic Resistance in Beef Cattle Manure of Open Feedlots

The objective of this study was to evaluate the effectiveness of lime amendment on the reduction of antimicrobial resistant bacteria and antimicrobial resistance genes in beef cattle manure in open feedlots. Hydrated lime was uniformly applied to the surface of feedlot pen floor 1 day prior to cattle harvest at a rate of 0.36 lb/ft2 and samples were collected over time. Collected samples were analyzed for change in pH and levels of antimicrobial resistant bacteria and antimicrobial resistance genes. Lime amendment elevated the pH of pen floor surface materials to pH \u3e 12 for 4 hours and then pH \u3e 11 for another 24 hours. Lime amendment reduced the concentration of generic and resistant Escherichia coli by 1–2 log for up to 4 hours. The abundance of antimicrobial resistance genes, such as tet(X) and tet(O), decreased by 1–2 order of magnitude with lime amendment. Results indicate that lime addition reduced the concentrations of antimicrobial resistant bacteria and antimicrobial resistance genes in pen surface materials from open beef feedlot pens

Inhibition of IFN-γ Signaling by an Epstein-Barr Virus Immediate-Early Protein

AbstractViruses have evolved elaborate mechanisms to target many aspects of the host's immune response. The cytokine IFN-γ plays a central role in resistance of the host to infection via direct antiviral effects as well as modulation of the immune response. In this study, we demonstrate that the Epstein-Barr virus (EBV) immediate-early protein, BZLF1, inhibits the IFN-γ signaling pathway. BZLF1 decreases the ability of IFN-γ to activate a variety of important downstream target genes, such as IRF-1, p48, and CIITA, and prevents IFN-γ-induced class II MHC surface expression. Additionally, BZLF1 inhibits IFN-γ-induced STAT1 tyrosine phosphorylation and nuclear translocation. Finally, we demonstrate that BZLF1 decreases expression of the IFN-γ receptor, suggesting a mechanism by which EBV may escape antiviral immune responses during primary infection

Identification of protein carbonyls in serum of the fetal and neonatal pig

Oxidation of serum proteins leads to non-reversible carbonyl formation which alters their function and is associated with stress-related disease processes. The primary objective of this study was to quantify and identify oxidized serum proteins in fetal and newborn piglets. Protein carbonyls were converted to hydrazones with dinitrophenyl hydrazine and quantified spectrophotometrically. For identification, serum protein carbonyls were derivatized with biotin hydrazide, separated by 2D PAGE and stained with FITCavidin. Biotin-labeled proteins were excised from gels and identified by mass spectrometry. At birth, carbonyls were determined to be ∼600 pmole/mg serum protein. Fetuses at 50 and 100 days of gestation had similar levels of protein carbonyls as newborns. Carbonyl levels were also similar for control and runt (birth) piglets between 1 and 21 days of age; however, distribution of many proteins varied by age and was also influenced by birth weight. Major oxidized proteins identified in fetal (f) and newborn (n) pigs included; albumin (f, n), transferrin (f, n), fetuin-A (f, n) alpha fetoprotein (f, n), plasminogen (f, n), fetuin-B (f), alpha-1-antitrypsin (f, n) alpha-1-acid glycoprotein (f) and immunoglobulins (n). While abundance and distribution of oxidized proteins changed over time, these changes appear to primarily reflect relative amounts of those proteins in serum

Retention of Swine Slurry Constituents in Soil and Crop Residue as Affected by Setback Distance

Setbacks are prescribed distances from surface waters within which manure application is not allowed. Little information is available concerning the retention of swine slurry constituents in soil and crop residue materials within setback areas. This study was conducted to measure the retention of selected constituents within a setback area following the upslope application of swine slurry and the introduction of simulated rainfall. The no-till cropland site had a slope gradient of 4.9% and a mean winter wheat residue cover of 7.73 Mg ha−1 . Soil and vegetative samples were collected on 3.7 m wide by 23.2 m long plots with and without the addition of slurry. Slurry was added at the 0–4.9 m distance on selected plots, and simulated rainfall was then applied to the entire plot area during two separate events. Soil cores and vegetative samples were collected from each plot at distances of 2.44, 5.18, 7.92, 11.0, 14.0, 17.1, and 20.1 m from the upper plot border. The soil cores were separated into 0–10, 10–20, and 20– 30 cm depth increments. Significant increases in soil concentrations of chloride, nitrate, phosphorus, and zinc were found both within and downslope from the slurry application area. Residue materials located both within and downslope from the slurry application area contained significantly increased concentrations of boron, calcium, copper, magnesium, sulfur, and zinc. When estimating the downslope transport of constituents contained in swine slurry, contributions from runoff, soil, and residue should all be considered

Setback Distance Requirements for Removal of Swine Slurry Constituents in Runoff

The use of setback distances for manure application on cropland areas adjacent to surface water bodies could serve a function similar to vegetative filter strips. However, little information currently exists to identify the setback distances necessary to effectively reduce the transport of contaminants in runoff. The objective of this study was to determine the effects of setback distance and runoff rate on concentrations of selected constituents in runoff following land application of swine slurry to a no-till cropland area in southeast Nebraska. The study site had a residue cover of 7.73 Mg ha-1 and a slope gradient of 4.9%. The twenty plots examined during the investigation were 3.7 m across the slope by 4.9, 7.9, 11.0, 17.1, or 23.2 m long. An initial set of rainfall simulation tests were completed to identify background concentrations of selected constituents. Swine slurry was then applied to the upper 4.9 m of each plot, and additional rainfall simulation tests were conducted on the same plots examined previously. A first-order exponential decay function was used to estimate the effects of setback distance on concentrations of selected constituents. A setback distance of 12.2 m reduced runoff concentrations of dissolved phosphorus (DP), NH4-N, total nitrogen (TN), boron, chloride, manganese, potassium, sulfate, zinc, electrical conductivity (EC), and pH to background values similar to those measured for the no-slurry condition. Runoff rate significantly influenced transport of several of the constituents, with concentrations generally decreasing as runoff rate increased. The transport of selected pollutants in runoff was significantly reduced when setback areas were employed

Resistome and mobilome in surface runoff from manured soil as affected by setback distance

Land application of livestock manure introduces antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) into the soil environment. The objectives of this study were to examine the changes of resistome and mobilome in runoff and soil as a function of setback distance, i.e., the distance between manured soil and surface water, and to quantify the contributions of manure and background soil to the ARGs and MGEs in surface runoff. The resistome and mobilome in runoff and soil from a field-scale plot study were characterized using a high throughput quantitative polymerase chain reaction (HT-qPCR) array. It was estimated that a setback distance of ~40 m is required to reduce the total abundance of ARGs and MGEs in runoff from amended plots to that in control runoff. The resistome and mobilome of the soil in the setback region was not affected by manure-borne ARGs and MGEs. SourceTracker analyses revealed that background soil gradually became the predominant source of the ARGs and MGEs in runoff as setback distance increased. The results demonstrate how manure-borne ARGs and MGEs dissipated in agricultural runoff with increasing setback distance and had limited impacts on the resistome and mobilome of soil within the setback region

How does facilitation in healthcare work? Using mechanism mapping to illuminate the black box of a meta-implementation strategy

BACKGROUND: Healthcare facilitation, an implementation strategy designed to improve the uptake of effective clinical innovations in routine practice, has produced promising yet mixed results in randomized implementation trials and has not been fully researched across different contexts. OBJECTIVE: Using mechanism mapping, which applies directed acyclic graphs that decompose an effect of interest into hypothesized causal steps and mechanisms, we propose a more concrete description of how healthcare facilitation works to inform its further study as a meta-implementation strategy. METHODS: Using a modified Delphi consensus process, co-authors developed the mechanistic map based on a three-step process. First, they developed an initial logic model by collectively reviewing the literature and identifying the most relevant studies of healthcare facilitation components and mechanisms to date. Second, they applied the logic model to write vignettes describing how facilitation worked (or did not) based on recent empirical trials that were selected via consensus for inclusion and diversity in contextual settings (US, international sites). Finally, the mechanistic map was created based on the collective findings from the vignettes. FINDINGS: Theory-based healthcare facilitation components informing the mechanistic map included staff engagement, role clarification, coalition-building through peer experiences and identifying champions, capacity-building through problem solving barriers, and organizational ownership of the implementation process. Across the vignettes, engagement of leaders and practitioners led to increased socialization of the facilitator\u27s role in the organization. This in turn led to clarifying of roles and responsibilities among practitioners and identifying peer experiences led to increased coherence and sense-making of the value of adopting effective innovations. Increased trust develops across leadership and practitioners through expanded capacity in adoption of the effective innovation by identifying opportunities that mitigated barriers to practice change. Finally, these mechanisms led to eventual normalization and ownership of the effective innovation and healthcare facilitation process. IMPACT: Mapping methodology provides a novel perspective of mechanisms of healthcare facilitation, notably how sensemaking, trust, and normalization contribute to quality improvement. This method may also enable more efficient and impactful hypothesis-testing and application of complex implementation strategies, with high relevance for lower-resourced settings, to inform effective innovation uptake