Development of a control system for laser doppler imaging scanning technique using labview

To monitor a small region of skin tissue for blood perfusion mapping construction, a Y-Z scanning control system using X-Y mechanism was developed. This control system utilizes the combination of a Stepper Motor and a DC Motor. A GCP (Graphical Coded Programming) of LabVIEW is used to compute the system driver controller via DAQ (Data Acquisition) card of PCI E-series. The results presented are based on experimental observation. Results show that the design is capable of performing the Y-Z scanning pattern up to 25 mm � 25 mm AOI (Area of Interest). © 2005 IEEE

Rotational kinetics of absorbing dust grains in neutral gas

We study the rotational and translational kinetics of massive particulates (dust grains) absorbing the ambient gas. Equations for microscopic phase densities are deduced resulting in the Fokker-Planck equation for the dust component. It is shown that although there is no stationary distribution, the translational and rotational temperatures of dust tend to certain values, which differ from the temperature of the ambient gas. The influence of the inner structure of grains on rotational kinetics is also discussed.Comment: REVTEX4, 20 pages, 2 figure

Antimicrobial substances of potential biomedical importance from holothurian species

161-164Antibacterial and antifungal activities of alcoholic extracts of holothurian species such as Actinopyga echinites, A. miliaris. Holothuria atra and H. scabra of Tamil Nadu coast were studied. Bacteria such as Aeromonas hydrophila, Escherichia coli, Enterococcus sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and Vibrio harveyi, and fish-borne mold, Aspergillus sp. were inhibited at varying levels by the extracts of A. miliaris, H. atra and H. scabra. Bacillus sp. was not affected by holothurian extracts. The results of the study revealed the presence of antimicrobial substances possibly steroidal sapogenins in holothuria. There exist a great potential for the extraction of bioactive substances of medical importance at a lower cost from marine holothurians

Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor

A single-pixel dry-type reconstituted bacteriorhodopsin (bR) sensor (Au/CMC + bR/ITO) incorporating carboxyrnethylcellulose (CMC) as the artificial membrane is fabricated according to a protocol developed to investigate signal acquisition from the proposed biosensor. Droplets of reconstituted purple membrane (PM) containing bR applied onto a gel-like solution of CMC placed on gold electrodes (anode) are allowed to dry under an electric field, utilising the PM fragment's net negative charge and electric dipole moment. The resulting bR matrix thin film with high molecular orientation is finally sandwiched together with a semi-transparent indium titanium oxide (ITO) counter electrode and proper electrical connections made to form a single pixel bR-CMC photosensor. Efficient and reproducible photoresponse observed upon illumination clearly exhibits the potential of the proposed biosensor for future photosensing works. (C) 2006 Elsevier B.V. All rights reserved

Distribution and abundance of luminous bacteria with special reference to shrimp farming activities

208-213Quantitative and qualitative distribution of planktonic luminous bacteria in aquaculturally affected region of Tuticorin Bay, along southeast coast of India was studied. Luminous bacterial abundance ranging from 20 to 1050 cells/ml in nearshore seawater and 100 to 8,150 cells/g inshore sediment were recorded. A significant positive correlation was observed between the luminous bacterial counts and total viable counts of seawater in areas having intensive shrimp farming activities. Five different species of luminous bacteria namely Vibrio fischeri, V. harveyi, V. orientalis, V. splendidus biotype 1 and Photobacterium leiognathi were identified during this study with V. harveyi as the dominant species, comprising ≥ 82-97 % of the total luminous population. These results suggest that intensive shrimp farming which largely contributed to luminous bacterial population strongly influences the distribution of planktonic luminous bacteria in nearshore seawater and probably alters the balance of autochthonous microflora in areas affected by shrimp farming

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Not AvailableSeasonal variation in the proximate composition (moisture, protein, fat, carbohydrate and ash) was analysed in emperor fish (Lethrinus lentjan). Mineral composition (iron, phosphorus, sodium, potassium and calcium) was also analysed. Moisture, protein, fat, carbohydrate and ash contents were found to range from 74.93–78.20, 14.53–24.40, 0.21–2.76, 0.49– 1.07 and 1.24–2.70% respectively. Iron, phosphorus, sodium, calcium and potassium was 4.20, 13.50, 7.20, 156.20 and 17.90 mg% respectively. Fatty acid profile of emperor fish revealed palmitic acid (C16), stearic acid (C18) and myristic acid (C14) to be the major saturated fatty acid and elaidic acid (C18:1n9T) and oleic acid (C18: 1n9C) were the dominant monounsaturated fatty acids. Investigations also revealed that fat content was higher during the monsoon season (October and November).Not Availabl

Identification of a novel 1,2 oxazine that can induce apoptosis by targeting NF-κB in hepatocellular carcinoma cells

10.1016/j.btre.2020.e00438Biotechnology Reports25e0043

N-substituted pyrido-1,4-Oxazin-3-ones induce apoptosis of hepatocellular carcinoma cells by targeting NF-κB signaling pathway

Hepatocellular carcinoma (HCC) is a fatal disease and ranked fifth in cancer related mortality. Persistent activation of NF-κB is responsible for the oncogenesis, metastasis, tumor evasion, anti-apoptosis, angiogenesis and proliferation in HCC. Therefore, designing of chemically novel, biologically potent small molecules that target NF-κB signaling cascade have gained prominent clinical interest. Herein we synthesized a novel class of 4-(substituted)-2H-pyrido[3,2-b][1,4]oxazin-3(4H)-one by reacting 2H-pyrido[3,2-b][1,4]oxazin-3(4H)-one with various alkyl halides by using combustion derived bismuth oxide. We evaluated the antiproliferative efficacy of newly synthesized compounds against HCC cells and identified 4-(4-nitrobenzyl)-2H-pyrido[3,2-b][1,4]oxazin-3(4H)-one (NPO) as lead anticancer agent. In addition, we investigated the effect of NPO on the DNA binding ability of NF-κB and NF-κB regulated luciferase expression in HCC cells. The results demonstrated that NPO can induce significant growth inhibitory effects in HepG2, HCCLM3 and Huh-7 cells in dose and time-dependent manner. Interestingly, NPO induced significant downregulation in p65 DNA binding ability, p65 phosphorylation and subsequent expression of NF-κB dependent luciferase gene expression in diverse HCC cell lines. Further, in silico docking analysis suggested that NPO can show direct physical interaction with NF-κB. Finally, NPO was found to significantly abrogate tumor growth at a dose of 50 mg/kg in an orthotopic mouse model. Thus, we report the potential anticancer effects of NPO as a novel inhibitor of NF-κB signaling pathway in HCC. Copyright © 2018 Mohan, Bharathkumar, Dukanya, Rangappa, Shanmugam

Fangchinoline, a bisbenzylisoquinoline alkaloid can modulate cytokine-impelled apoptosis via the dual regulation of NF-?B and AP-1 pathways

10.3390/molecules24173127Molecules2417312

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Not AvailableBackground: The success of larval rearing is greatly influenced by first feeding regimes and the nutritional quality of weaning diets, with dietary lipids being recognized as one of the most important nutritional factors that affect larval growth and survival. Reports are scanty on milkfish larval nutrition and growth and survival unlike other marine species. Methods: In this investigation during 2020, five larval diets were prepared with 40 g kg-1 of entirely fish oil (F4), corn oil (C4) or fish oil and corn oil in 3:1 (F3C1), 2:2 (F2C2) and 1:3 (F1C3) ratios. Each diet was fed to triplicate groups of milkfish larvae (45 mg) in a flow-through rearing system for 42 days. Result: A growth indices were highest in the F3C1 group, followed by F2C2, F4 and other dietary treatments. The whole-body fatty acid profile was found to change significantly with increasing fish oil replacement with corn oil, i.e., the n-3 polyunsaturated and saturated fatty acid proportions decreased linearly, while the n-6 polyunsaturated fatty acids content increased. Overall, this study reveals that growth and survival of milkfish is dependent on dietary lipid source or combinations that meet the essential fatty acid requirements during the early life stages.Not Availabl