Sample-to-sample variation in the effect of seminal plasma on tight junctional proteins.

<p>Bars represent mean abundance ± standard error of specific tight junctional proteins (Claudin-2 [panel A], Claudin-4 [panel B], Claudin-5 [panel C] and Claudin-7 [panel D]) relative to the control condition, for 4 cell layers (3 cell layers for sample D). Asterisks indicate level of statistical difference (Student’s t test, two-tailed), relative to control: * P < 0.05, ** P < 0.01, *** P < 0.001. # P < 0.05 vs Samples A and C vs Sample D.</p

Summary of effects of specific seminal plasma components on CACO-2 cell layer barrier function.

<p>Summary of effects of specific seminal plasma components on CACO-2 cell layer barrier function.</p

Sample-to-sample variation in Interleukin-8 (A) and zinc (B) concentrations in seminal plasma.

<p>Seminal plasma was diluted 1:20 in culture medium. IL-8 and zinc levels were measured as described in Materials and Methods. Each bar is the average of two (IL-8) or five (zinc) measurements with a variation of less than 5% of the mean.</p

Specific Components of Seminal Plasma in Substantial Excess Relative to Blood Plasma

<p>Specific Components of Seminal Plasma in Substantial Excess Relative to Blood Plasma</p

Zinc reduces epithelial barrier compromise induced by human seminal plasma - Fig 5

<p><b>Effect of zinc chelation on seminal plasma action on transepithelial electrical resistance (A) and transepithelial mannitol permeability (B).</b> Error bars represent standard error of the mean for n = 3 cell layers. * indicates P < 0.05, ** P < 0.01 for Student’s t-test, two-tailed, relative to control bar. # P = 0.05 relative to SP condition. Experiment was repeated 3 times with similar results.</p

Rhesus Macaque TRAV and TRDV genes

TRAV and TRDV genes extracted from the rhesus macaque genome. The gene features reported include L-PART1, V-INTRON, V-EXON, V-HEPTAMER, V-SPACER, and V-NONAMER. The FASTA header fields include the species name, rhesus macaque chromosome 7 accession number (NC_007864.1), start and end positions of the gene feature in the rhesus macaque chromosome 7, strand (-1 indicates that the reported sequences are the reverse complement), gene name, and gene feature

Rhesus Macaque TRAJ genes

TRAJ genes extracted from the rhesus macaque genome. The gene features reported include J-NONAMER, J-SPACER, and J-HEPTAMER. The FASTA header fields include the species name, rhesus macaque chromosome 7 accession number (NC_007864.1), start and end positions of the gene feature in the rhesus macaque chromosome 7, strand (-1 indicates that the reported sequences are the reverse complement), gene name, and gene feature