Preparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albumin

WOS: 000244617500008Novel sulfonyl-hydrazine carrying poly(styrene-divinylbenzene), poly(S-DVB)-S-NH2 ion-exchange beads (size between 210 and 425 mu m) were prepared via suspension polymerization and, were first used as an ion-exchange support for adsorption of human serum albumin (HSA) from aqueous solution. The influence of pH, equilibrium time, ionic strength and initial albumin concentration on the adsorption capacity of the poly(SDVB)-S-NH2 ion-exchange beads has been investigated in a batch system and the unmodified poly(S-DVB) beads were used as control system. Maximum HSA adsorption onto poly(S-DVB)-S-NH2 ion-exchange beads was found to be 63.05 mg/g at pH 7.0. The experimental equilibrium data for HSA adsorption onto poly(S-DVB)-S-NH2 beads was well described by the Langmuir isotherm model. Kinetic parameters of this adsorption system were also analyzed for HSA adsorption onto beads and, the first-order rate equations were favorable. Finally, the poly(S-DVB)-S-NH2 beads was used for the purification of HSA from whole human serum and, the purity of the eluted HSA from the beads was determined as 89% by HPLC from single step purification protocol. (c) 2006 Elsevier B.V. All rights reserved

A radioactively durable melamine-styrene based polymer: Highly efficient removal of Sr-90

Removal of strontium has a vital importance for radioactive waste management due to its long half-life. In this context, melamine-styrene based polymer (MSBP) was synthesized and characterized by different spectrophotometric methods. Sr2+ ions were removed from the solution using MSBP sorbent. In this respect, adsorption of Sr(2+)onto melamine-based polymer was studied as a part of pH, amount of adsorbent, initial Sr(2+)concentration, contact time, temperature and particle size. Also, adsorption rate of radioactive strontium was investigated by using LSC (Liquid Scintillation Counter). The adsorption data were fitted to Langmuir, Freundlich and Dubinin-Radushkevich (D-R) isotherm models. MSBP sorbent reached maximum adsorption capacity for Sr2+ as 142.9 mg.g(-1). Thermodynamic parameters such as free energy (Delta G(0)), entropy (Delta S-0) and enthalpy (Delta H-0) of Sr2+ adsorption on MSBP were examined at five different temperatures of 293 K, 303 K, 313 K, 323 K and 333 K. 0 Delta G(0) and 0 < Delta S-0 revealed that Sr2+ adsorption on MSBP occurred spontaneously and irreversible, respectively. Pseudo first and second orders were investigated as a part of kinetic parameters and seen that pseudo second order was much more convenient for adsorption of Sr2+ onto MSBP

Synthesis of New Polymeric Resin and Its Application in Solid Phase Extraction of Copper in Water Samples Using STAT-FAAS

In this study, thiol-containing sulfonamide resin was synthesized, characterized, and applied as a new sorption material for the determination of copper in water samples. The method, solid phase extraction, is based on the sorption of Cu ions on the synthesized resin without using any complexing reagent. The optimization of experimental conditions was performed by changing parameters including pH, contact time, initial sample volume, and volume of eluent. Flame atomic absorption spectrometry (FAAS) was used for the determinations. The optimized method includes advantages such as a rapid and simple preconcentration step compared to other similar methods because there is no need for a complexing reagent, and the optimum pH of the solution is found in acidic media (pH=3). In brief, 120-fold improvements in the sensitivity of FAAS were achieved by combining slotted tube atom trap-atomic absorption spectrometry (STAT-FAAS) and the optimized preconcentration method. To examine the reliability, the method was checked by using both standard reference materials and ICP-MS measurements. The Cu concentrations in the water samples were found to be in the range of 0.9-13.0 ng m L-1WOS:0002809721000022-s2.0-7574912578

The effect of liver test abnormalities on the prognosis of COVID-19

Introduction: COVID-19 caused by the SARS-CoV-2 continues to spread rapidly across the world. In our study, we aim to investigate the relationship between the liver enzymes on admission (AST, ALT, ALP, GGT) and severity of COVID-19. We evaluated course of disease, hospital stay, liver damage and mortality. Materials and methods: Our study included 614 patients who were hospitalized with the diagnosis of COVID-19 between 03.16.20 and 05.12.20. Patients with liver disease, hematological and solid organ malignancy with liver metastases were excluded, resulting in 554 patients who met our inclusion criteria. We retrospectively evaluated liver transaminase levels, AST/ALT ratio, cholestatic enzyme levels and R ratio during hospital admission and these were compared in terms of morbidity, mortality and clinical course. Results: Mean age of 554 subjects were 66.21 ± 15.45 years, 328 (59.2%) were men. The mean values of liver enzymes on admission were AST (36.2 ± 33.6 U/L), ALT (34.01 ± 49.34 U/L), ALP (78.8 ± 46.86 U/L), GGT (46.25 ± 60.05 U/L). Mortality rate and need for intensive care unit were statistically significant in subjects that had high ALT–AST levels during their admission to the hospital (p = 0.001). According to the ROC analysis AST/ALT ratio was a good marker of mortality risk (AUC = 0.713: p = 0.001) and expected probability of intensive care unit admission (AUC = 0.636: p = 0.001). R ratio, which was used to evaluate prognosis, showed a poor prognosis rate of 26.5% in the cholestatic injury group, 36.1% in the mixed pattern group and 30% in the hepato-cellular injury group (p 0.001). Conclusions: ALT–AST elevation and AST/ALT ratio >1 was associated with more severe course and increased mortality in COVID-19