The effects of slaughtering methods on physical and biochemical changes in fish

Duran, Ayhan (Aksaray, Yazar)We investigated the effects of two different slaughter methods (sudden death and struggle death) on catalase (CAT) activity and malonedialdehyde (MDA) levels in tissues of rainbow trout (Oncorhynchus mykiss Walbaum, 1792). We analyzed a group of fish without waiting and other fish groups after being kept in 8-10°C for 12, 24, 36 hours. Additionally, sensory analyses were studied by a sensory panel consisting of experienced judges to sensory evaluation. We have been observed significant increases (p < 0.05) in MDA levels and decreases (p < 0.05) in CAT activity of two groups (sudden and struggle death). Using sudden death (unstressed) method, rainbow trout groups showed good quality until to 36th hour, and sensory analyses of struggle death treated groups (stressed) exhibited good quality up to 24th hour. Consequently, we got importance of storage temperature, time and different slaughter methods on biochemical parameters and sensory analyses of some rainbow trout tissues

Biochemical changes and sensory assessment on tissues of carp (Cyprinus carpio, Linnaeus 1758) during sale conditions

WOS: 000270171000017PubMed: 19533401In this study, some biochemical changes of carp (Cyprinus carpio, Linnaeus 1758) tissues were investigated. Studies have been carried out on carp which have regional economical importance. Storage temperature and time are the most important factors that affect the quality of fish during sales. It was observed that the temperature varied between 9 and 12A degrees C in sale conditions. In addition, we assumed the arrival time of the fish at the fish market to be 0 (zero) h. Biochemical analyses [malondialdehyde (MDA) levels and catalase activity] of carp tissues (muscle, liver, heart, spleen, brain) were carried out on fish which were held for 24 and 48 h, as well as on fresh fish (0 h). In addition, sensory analysis was conducted by a panel consisting of experienced judges of sensory evaluation. Statistically significant (P 0.05) increase in MDA level in heart tissue of carp after 24 h. There was a statistically significant (P < 0.05) increase in MDA levels in muscle, spleen and heart tissues when comparing the 24- and 48-h groups. In the group examined at 24 h, it was observed that there were statistically significant differences from the 0 h group values (P < 0.05) for catalase (CAT) activity in muscle, brain, spleen and heart tissues. The decreases in CAT activity in liver and spleen tissues were found to be statistically significant (P < 0.05) between the group examined at 24 h compared with the group examined at 48 h. Carp maintained good quality during the selling conditions up to 24 h. This experiment deals with the effects of post-slaughter time and storage temperature on carp tissues. It is concluded that by considering the storage temperature (9-12A degrees C) and storage time (post-slaughter) the product maintained acceptable quality up to 24 h. There was significant deterioration of sensory quality, as a result of changes in chemical constituents.Inonu University Research Fund [2003/08]Inonu University Research Fund (I. U. BAP 2003/08) is gratefully acknowledged for support of this work

Effects of propolis on blood biochemical and hematological parameters in nitric oxide synthase inhibited rats by N omega-Nitro-L-arginine methyl ester

WOS: 000325830800009PubMed: 24035946This study showed the effects of propolis on biochemical and hematological parameters in chronic nitric oxide synthase inhibited rats by N-omega-Nitro-L-arginine methyl ester (L-NAME). Rats are given L-NAME for 15 days and the propolis for the last 5 days with L-NAME together. The levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and gamma glutamyltransferase in the L-NAME group compared to control group have increased (P<0.05). The levels of these parameters in L-NAME+propolis group compared to the L-NAME group have decreased (P<0.05). L-NAME caused increase (P<0.05) in levels of glucose, albumin, globulin, creatinine, urea, triglyceride and cholesterol. Erythrocyte number, total leukocyte, hemoglobin, hematocrit, neutrophil and monocyte decreased (P<0.05), platelets and lymphocyte increased (P<0.05) in L-NAME+propolis group compared to the L-NAME group. The study concluded that homeostasis is modulated in L-NAME administrated rats by adding propolis which causes increasing generation of vascular nitric oxide.Nigde University Research Fund [FEB 2008/25]Nigde University Research Fund (FEB 2008/25) is gratefully acknowledged for support of this work. Thanks for his help to Dr. Engin Sahna at Firat University in Turkey about animal research

Biologic Activities of Honeybee Products Obtained From Different Phytogeographical Regions of Turkey

Honeybee products are rich in phenolic compounds, which effect as natural antioxidants. These compounds may be attached as indicators in studies into the floral and geographical origin of the natural bee products. In this study, we aimed to determine average total antioxidant capacity, average total oxidant capacity and average oxidative stress index of natural bee products obtained from different regions of Turkey. Collected honeybee samples were kept at +4o C until extracted. Natural bee products were extracted with specific methods and antioxidant capacities were defined with in vitro analyses and data were compared. As a result, the highest average total antioxidant capacities were observed in propolis and pollen samples. Total antioxidant capacities of honeybee products collected from various regions demonstrated differences (P&lt;0.05) because of different phytogeographical characteristics of regions of Turkey.Honeybee products are rich in phenolic compounds, which effect as natural antioxidants. These compounds may be attached as indicators in studies into the floral and geographical origin of the natural bee products. In this study, we aimed to determine average total antioxidant capacity, average total oxidant capacity and average oxidative stress index of natural bee products obtained from different regions of Turkey. Collected honeybee samples were kept at +4o C until extracted. Natural bee products were extracted with specific methods and antioxidant capacities were defined with in vitro analyses and data were compared. As a result, the highest average total antioxidant capacities were observed in propolis and pollen samples. Total antioxidant capacities of honeybee products collected from various regions demonstrated differences (P&lt;0.05) because of different phytogeographical characteristics of regions of Turkey

The effects of propolis on gill, liver, muscle tissues of rainbow trout (Oncorhynchus mykiss) exposed to various concentrations of cypermethrin

The aim of this study was to investigate the therapeutic effects of propolis for the toxicity of cypermethrin (CYP) on histopathological changes in tissues of rainbow trout (Oncorhynchus mykiss). CYP is one of the most toxic pyrethroids highly for the aquatic organisms. The fish were exposed to three sublethal concentrations of CYP (0.0041, 0.0082 and 0.0123 ppm). In addition, different concentrations of propolis (10, 20 and 30 ppm) were used in the investigation for the period of 96 h. Propolis was collected by honeybees from different plants to prevent oxidative damages as an antioxidant. The therapeutic concentration of propolis was determined at 10 ppm. 10 ppm propolis was added to three cypermethrin concentrations. Shorting the secondary lamellae , fusion of secondary lamellae, oedema, necrosis, vacuolization and cartilage damage in gill tissue of fish exposed to CYP were observed by histopatological analyses. Hepatic lesions in liver tissue of fish exposed to CYP were characterized as hydropic degeneration, necrosis, mononuclear cell infiltration and narrowing of sinusoids. The most common changes in muscle tissues at all concentrations of CYP were nuclear proliferation and congestion. Besides, 10 ppm propolis on gill, liver, muscle tissues of rainbow trouts showed significant therapeutic effects. Histological analysis revealed that propolis may serve as an antitoxic agent against pesticide toxicity

Effects of propolis on biochemical and microbiological parameters in carp (Cyprinus carpio) fillets exposed to arsenic

The purpose of this study was to investigate the therapeutic effects of propolis on biochemical and microbiological parameters in muscle tissues of carp (Cyprinus carpio, Linnaeus 1758) exposed to arsenic. A sublethal concentration of arsenic (0.01 ppm) and/or 0.01 ppm propolis were administered to fish for seven days. Biochemical parameters [pH, lactic acid, total volatile basic nitrogen (TVB-N), and malondialdehyde (MDA) values] and microbiological changes (mesophilic and psychrophilic bacteria count) were determined in fillet of carp in control, arsenic only, propolis only and arsenic+propolis treatment groups. Results showed that the levels of MDA, lactic acid and TVB-N increased (p<0.05) while there were decreases total counts of psychrophilic, mesophilic bacteria and level of pH in arsenic group compared to the control group. Additionally, levels of pH, lactic acid, TVB-N, counts of psychrophilic and mesophilic bacteria in arsenic (0.01 ppm)+propolis (10 ppm) group significantly reduced compared to arsenic group (p<0.05). In conclusion, propolis can affects some biochemical and microbiologic functions and quality in the fillet of carp exposed to arsenic

Antioxidative role of selenium on some tissues of (Cd2+, Cr3+)-induced rainbow trout

WOS: 000258550400009PubMed: 17920681Chemical toxic pollutants (especially heavy metals) are important sources of reactive oxygen species (ROS) in biological systems. Membrane phospholipids of aerobic organisms are continually subjected to oxidant challenges from endogenous and exogenous sources, while peroxidized membranes and lipid peroxidation products represent constant threats to aerobic cells. The primary antioxidant protection against free radical and ROS is provided by the enzymes glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT), respectively. The trace element selenium has been implicated in chemo-prevention and drug-resistance through reduction of oxidative stress. Selenium could prevent damage to the unsaturated fatty acid Of subcellular membranes by lipid peroxidation induced by free radicals. The results reported here show that sodium selenite has an important contribution to antioxidative defense for the spleen and heart of rainbow trout. The ability of sodium selenite to prevent the oxidative stress induced by heavy metals (Cd2+, Cr3+) in fish was rationalized. (C) 2007 Elsevier Inc. All rights reserved.Inonu University Research Fund [BAP 2004/27]This work was supported by Inonu University Research Fund (BAP 2004/27)

Effects of sodium selenite on some biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) exposed to Pb2+ and Cu2+

WOS: 000253527500007PubMed: 18649023This study was carried out to understand the preventive effect of selenium (Se4+) on heavy metal stress induced by lead and copper in rainbow trout (Oncorhynchus mykiss). Variation in glutathione peroxidase (Se-GSH-Px) and superoxide dismutase (SOD) activity, and in malondialdehyde (MDA) levels in liver, spleen, heart, and brain tissues of rainbow trout after 72 h of exposure to Pb2+ and Cu2+ were investigated in the presence and absence of Se4+. In the presence of Se4+, Se-GSH-Px activity and SOD activity were found to be higher and MDA levels were lower compared with in its absence. Hematological parameters were also determined and it has been observed that total leukocyte count (WBC), mean cell volume (MCV), and mean cell hemoglobin (MCH) were increased and erythrocyte number (RBC), hemoglobin (Hb), and hematocrit value (Hct; P < 0.05) were decreased in fish exposed to heavy metals in the absence of selenium. Selenium presence recovered hematological parameters to normal levels. In the light of our findings, it could be stated that Pb2+ and Cu2+ lead to dramatic changes in biochemical and hematological parameters and selenium caused these parameters to converge to control levels when it was administered concurrently with these heavy metals

Antioxidative role of selenium against the toxic effect of heavy metals (Cd+2, Cr+3) on liver of rainbow trout (Oncorhynchus mykiss Walbaum 1792)

WOS: 000258086500004PubMed: 18665459The main purpose of this study is to discuss the effect of Cd+2, Cr+3 and Se metals on biochemical parameters in liver tissue of Oncorhynchus mykiss. The rainbow trout were exposed to heavy metal stress ( Cd+2, Cr+3) at 2 ppm dosage. The present study was undertaken to determine the protective effect of selenium treatment at the same dosage ( 2 ppm) on some biochemical parameters. The activity of catalase ( CAT), glutathione peroxidase ( GSH-Px), superoxide dismutase ( SOD) and the changes in levels of malondialdehyde ( MDA) from biochemical parameters were determined in liver tissue of the fish groups exposed to heavy metals, especially for the selenium-applied groups. Results of this study showed that the activities of CAT, GSH-Px and SOD in the tissues of fish exposed to the stress of Cd+2 and Cr+3 were significantly lower than the control groups ( P < 0.05). Meanwhile, the closer values to the control groups were obtained in selenium-added groups ( Cr+3 + Se+4, Cd+2 + Se+4). For the level of MDA, the last production of lipid peroxidation showed increases ( P < 0.05) in the groups exposed to the metal stress, whereas significant decreases were obtained in selenium-applied groups. The result of the statistical evaluation showed that the negative effects occurring in the biochemical parameters of the applied groups exposed to the toxicity of heavy metal were significantly eliminated ( P < 0.05) as a result of selenium treatment

Effects of Propolis on microbiologic and biochemical parameters of Rainbow trout (Oncorhynchus mykiss) after exposure to the pesticide

Cypermethrin is a potential toxic pollutant that directly threatens the aquatic ecosystems and environment. Propolis, a natural product collected from plants by honey bees has many functions, such as antimicrobial, antioxidant, and anti-inflammatory. This study attempted to detrmine some biochemical and microbiological parameters of rainbow trout fillets after exposure to propolis and cypermethrin. The results of the current study revealed that levels of malondialdehyde (MDA), lactic acid, total volatile base-nitrogen (TVB-N), total counts of psychrophilic, and mesophilic bacteria increased in cypermethrin groups (P<0.05), compared to control group. Furthermore, there were no significant differences in pH levels between groups. Additionally, levels of MDA, lactic acid, TVB-N, count of psychrophilic, and mesophilic bacteria in cypermethrin+propolis treated groups were significantly reduce in comparison to exposed groups with cypermethrin (P<0.05). The findings also showed that the fillet quality some biochemical and microbiologic functions of fishes could be changed in groups that exposed to cypermethrin by propolis