Flow chart of experimental treatment groups.

<p>Flow chart of experimental treatment groups.</p

Infection, clearance and survival rates of each treatment group (excluding unexposed controls) following exposure to <i>Batrachochytrium dendrobatidis</i> (<i>Bd</i>).

<p>“Inoculated” frogs were previously exposed and infected with <i>Bd</i> and cleared of infection with itraconazole prior to exposure, “Treated” frogs were not initially exposed to <i>Bd</i> but were treated with itraconazole prior to exposure, “Naïve” frogs were not exposed nor treated prior to exposure.</p

Dataset 5.2 Liver-filtered-condensed-transdecoder

Dataset 5 consists of de novo assembled transcriptomes for each tissue type from frogs in Experiment B (n = 61). These data are contained in Dataset 5, as six individual .fasta text files, including both nucleotide sequence assembly files and amino acid sequence assembly files (translated using TransDecoder) for each of the three tissue types (skin, liver and spleen) [Data Citation 1]. Descriptions of the sequence identifier lines are contained in the associated readme file

Dataset 6.1 Liver transcriptome functionally annotated

Dataset 6 consists of functional annotation data (from the Gene Ontology [GO] consortium, Enzyme Code and Inter Pro databases) for each gene assembled, across the three tissue types (skin, liver and spleen). These data are contained in Dataset 6, as three individual comma-separated files (.csv format) [Data Citation 1]. Full descriptions of the column headers are contained in the associated readme file

Dataset 7.7 Skin matrix.isoforms.TPM.not_cross_norm

Dataset 7 contains the raw gene/transcript counts data for each frog tissue sample (Experiment B, n = 61) resulting from allocation of sequence reads to genes from the transcriptome assemblies. These data are contained in Dataset 7, as 12 individual tab-separated text files (.txt) [Data Citation 1]. The four files for each tissue type (skin, liver and spleen) represent raw (1) counts, (2) TMM.EXPR files, (3) TPM.not_cross_norm files, and (4) normalisation information (as per the standard output from RSEM). The first row of these files (1-3 above) represents frog ID, and the first element of all subsequent rows represents transcript/isoform ID

Dataset 7.3 Liver matrix.isoforms.TPM.not_cross_norm

Dataset 7 contains the raw gene/transcript counts data for each frog tissue sample (Experiment B, n = 61) resulting from allocation of sequence reads to genes from the transcriptome assemblies. These data are contained in Dataset 7, as 12 individual tab-separated text files (.txt) [Data Citation 1]. The four files for each tissue type (skin, liver and spleen) represent raw (1) counts, (2) TMM.EXPR files, (3) TPM.not_cross_norm files, and (4) normalisation information (as per the standard output from RSEM). The first row of these files (1-3 above) represents frog ID, and the first element of all subsequent rows represents transcript/isoform ID

Dataset 7.2 Liver matrix.isoforms.TMM.EXPR

Dataset 7 contains the raw gene/transcript counts data for each frog tissue sample (Experiment B, n = 61) resulting from allocation of sequence reads to genes from the transcriptome assemblies. These data are contained in Dataset 7, as 12 individual tab-separated text files (.txt) [Data Citation 1]. The four files for each tissue type (skin, liver and spleen) represent raw (1) counts, (2) TMM.EXPR files, (3) TPM.not_cross_norm files, and (4) normalisation information (as per the standard output from RSEM). The first row of these files (1-3 above) represents frog ID, and the first element of all subsequent rows represents transcript/isoform ID

Dataset 7.1 Liver matrix.isoforms.counts

Dataset 7 contains the raw gene/transcript counts data for each frog tissue sample (Experiment B, n = 61) resulting from allocation of sequence reads to genes from the transcriptome assemblies. These data are contained in Dataset 7, as 12 individual tab-separated text files (.txt) [Data Citation 1]. The four files for each tissue type (skin, liver and spleen) represent raw (1) counts, (2) TMM.EXPR files, (3) TPM.not_cross_norm files, and (4) normalisation information (as per the standard output from RSEM). The first row of these files (1-3 above) represents frog ID, and the first element of all subsequent rows represents transcript/isoform ID

Dataset 7.9 Spleen matrix.isoforms.counts

Dataset 7 contains the raw gene/transcript counts data for each frog tissue sample (Experiment B, n = 61) resulting from allocation of sequence reads to genes from the transcriptome assemblies. These data are contained in Dataset 7, as 12 individual tab-separated text files (.txt) [Data Citation 1]. The four files for each tissue type (skin, liver and spleen) represent raw (1) counts, (2) TMM.EXPR files, (3) TPM.not_cross_norm files, and (4) normalisation information (as per the standard output from RSEM). The first row of these files (1-3 above) represents frog ID, and the first element of all subsequent rows represents transcript/isoform ID

Dataset 4 Sequencing quality control data

Dataset 4 consists of a summary of the phred Q-score quality control data accompanying the sequence data from each tissue sample (described above). These data are contained in Dataset 4 as a single comma-separated file (.csv format). Descriptions of the column headers are contained in the associated readme file