Gene TNFA em pacientes brasileiros com acidente vascular encefálico hemorrágico ou aneurisma cerebral

Introdução: Muitas doenças cerebrovasculares relacionam-se com processos inflamatórios, portanto, a influência de vários polimorfismos em doenças tem sido estudada para melhorar o conhecimento sobre os mecanismos fisiológicos do sistema nervoso. Objetivo: Identificar a associação entre um polimorfismo na posição -308 do gene TNFA e o desenvolvimento de acidente vascular encefálico hemorrágico (AVEH) ou aneurisma em pacientes de uma base hospitalar do Distrito Federal, Brasil. Métodos: Foram coletados os prontuários e as informações clínicas de pacientes com AVEH ou aneurisma. A caracterização dos grupos caso foi confirmada por tomografia computadorizada (TC) ou ressonância nuclear magnética (RNM). Os genótipos do gene TNFA foram determinados por técnica do polimorfismo de comprimento dos fragmentos de restrição do produto obtido pela reação em cadeia da polimerase (PCR). Resultados: O genótipo AG parece diminuir a ocorrência de AVEH ou aneurisma em indivíduos entre 45 e 63 anos. Nosso estudo foi o primeiro a investigar essa associação em uma amostra brasileira, embora um relatório anterior tenha mostrado efeito semelhante com o acidente vascular encefálico isquêmico em uma população chinesa. Conclusão: O genótipo TNFA -308 AG está associado à diminuição do risco de aneurisma ou AVEH em uma população da capital do Brasil, Distrito Federal.Introduction: Many cerebrovascular diseases display a relation with inflammatory processes. Furthermore, the influence of several polymorphisms has been studied to improve the knowledge of physiological mechanisms of the nervous system. Objectives: The aim of this study was to identify if there was an association between a polymorphism in -308 position of the TNFA gene and the development of hemorrhagic stroke or aneurysm in Distrito Federal, Brazil. Methods: We collected the clinical information and the medical records from hemorrhagic stroke or aneurysm patients. The occurrence of stroke or aneurysm was confirmed by computed tomography (CT) or magnetic resonance image (MRI). The TNFA genotypes were determined by polymerase chain reaction restriction fragment length polymorphism. Results: The AG genotype appears to decrease the occurrence of hemorrhagic stroke or aneurysm in people between 45-63 years. Our study was the first to investigate this association in a Brazilian sample, although a previous report showed a similar effect with ischemic stroke in a Chinese population. Conclusion: The TNFA -308 AG genotype is associated with a decreased risk of aneurysm or hemorrhagic stroke in a population from the capital of Brazil, Distrito Federal

The impact of sarcopenic obesity on inflammation, lean body mass, and muscle strength in elderly women

Dahan da Cunha Nascimento,1,2 Samuel da Cunha Oliveira,1 Denis Cesar Leite Vieira,2,3 Silvana Schwerz Funghetto,4 Alessandro Oliveira Silva,5,6 Renato Valduga,7 Brad Jon Schoenfeld,8 Jonato Prestes1 1Department of Physical Education, Catholic University of Brasilia (UCB), Brasilia, Brazil; 2Department of Physical Education, University Center of the Federal District (UDF), Brasilia, Brazil; 3Department of Physical Education, University of Brasilia (UNB), Brasilia, Brazil; 4Department of Nursing, University of Brasilia (UNB), Brasilia, Brazil; 5University Center of Brasilia (UniCEUB), Brasilia, Brazil; 6Department of Medicine and Physical Education, Integrated Colleges of the Central Plateau Educational Union (FACIPLAC), Brasilia, Brazil; 7Department of Physiotherapy, Secretary of State for Health of the Federal District, Emergency Unit, Ceilandia Regional Hospital, Brasilia, Brazil; 8Department of Health Sciences, CUNY Lehman College, Bronx, NY, USA Objective: The objective of this study was to apply the newly standardized definition for sarcopenia from the Foundation for the National Institutes of Health (FNIH) and the current definition for obesity to 1) determine the prevalence of sarcopenic obesity (SO) in obese elderly women; 2) compare the muscle strength, lean body mass, and markers of inflammation between obese elderly women with SO and nonsarcopenic obesity (NSO), and 3) elucidate the relationship between appendicular lean mass adjusted for body mass index (aLM/BMI) with muscle strength, lean body mass, and obesity indices. Methods: A total of 64 elderly obese women (age: 68.35±6.04 years) underwent body composition analysis by dual-energy X-ray absorptiometry. Participants were classified into two groups according to the definition of SO and NSO. Blood samples were collected for total cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein, uric acid, urea, interleukin-6 (IL-6), glucose, and creatine kinase (CK) measurements. Results: The SO group presented a significantly greater BMI, fat (%), glucose, a marginal trend toward significance for uric acid, and IL-6 compared to the NSO group. In addition, the SO group displayed lower values for muscle strength and lean body mass. From a correlation standpoint, a higher aLM/BMI was positively associated with lean body mass and muscle strength and negatively associated with a lower BMI and percentage body fat. Conclusion: The definition criteria from FNIH and obesity permit the ability to illustrate the prevalence and identify SO in elderly women with low muscle mass, low muscle strength, and impaired markers of inflammation. Keywords: aging, fat mass, muscles, obesity, sarcopenia, sarcopenic obes

Blood pressure response to resistance training in hypertensive and normotensive older women

Dahan da Cunha Nascimento,1,2 Cristiane Rocha da Silva,1 Renato Valduga,1,3 Bruno Saraiva,1 Ivo Vieira de Sousa Neto,4 Amilton Vieira,4 Silvana Schwerz Funghetto,4 Alessandro Oliveira Silva,5 Samuel da Cunha Oliveira,1 Guilherme Borges Pereira,1 Jefrey M Willardson,6 Jonato Prestes11Programa de Pós-Graduação em Educação Física, Universidade Católica de Brasília, Brasília, Brazil; 2Centro Universitário do Distrito Federal (UDF), Brasília, Brazil; 3Centro Universitário Unieuro, Brasília, Brazil; 4Universidade de Brasília (UNB), Brasília, Brazil; 5Centro Universitário de Brasília (UNICEUB), Brasília, Brazil; 6Health and Human Performance Department, Rocky Mountain College, Billings, MT, USAPurpose: The purpose of the present study was to identify the variability of blood pressure response to a 10-week resistance training (RT) program in hypertensive and normotensive elderly women.Participants and methods: Twenty-seven untrained hypertensive and 12 normotensive elderly women participated in the present study. A whole-body RT program was performed on two nonconsecutive days per week for 10 weeks. The responsiveness of resting systolic blood pressure (SBP) was determined based on the percent decline between the pre- and post-training time points T1 and T4. The term responders were used to describe subjects who exhibited a percent SBP decline ≥−2.58% and the term nonresponders for subjects who exhibited a percent SBP decline <−2.58%, respectively.Results: Both the responders and nonresponders in the hypertensive group presented significant changes in SBP (−7.83 ± 5.70 mmHg vs 3.78 ± 7.42 mmHg), respectively. Moreover, the responders and nonresponders in the normotensive group presented significant changes in SBP as well (−8.58 ± 5.52 mmHg vs 5.71 ± 3.84 mmHg).Conclusion: SBP presents a heterogeneous response to a controlled RT program in hypertensive and normotensive elderly women. A different modality of training and additional therapies should be used for nonresponders in order to decrease resting SBP.Keywords: resistance training, exercise, hypertension, responsiveness, elderly, obesit