Editorial: IPPS 2022 - plant phenotyping for a sustainable future

Plants are a venue for addressing the challenges facing humanity. The need for a reliable supply of food, feed, materials, chemicals and energy as well as ways to manage agroecology and climate change are among the challenges that we can address through the sustainable use of plants and plant ecosystems. The research community needs to integrate plant systems approaches, from molecular to organismal to applications in the field and ecosystems, to increase productivity sustainably while using fewer land, water, and nutrient resources. In the past two decades, plant phenotyping research has developed a highly valuable portfolio of technologies, processes and infrastructures to address these questions (Pieruschka and Schurr, 2019). In the past, the creation of datasets was limited by low throughput sensing and image analysis (Tsaftaris et al., 2016). However, through the development of digital image analysis the previous phenotyping “bottleneck” has shifted towards a capacity problem, making it difficult to interpret vast datasets (especially in the face of plant x environment interactions), leading to an “interpretation bottleneck” (Smith et al., 2021). Innovative plant phenotyping approaches that reveal and target relevant traits are thus still needed to identify and quantify key traits and processes and to understand the dynamic interactions between genetics, molecular and biochemical processes, and the physiological responses to changes in the environment that lead to the development of a phenotype

Seismotectonic study of the Fergana region (Southern Kyrgyzstan): distribution and kinematics of local seismicity

We present new seismicity and focal-mechanism data for the Fergana basin and surrounding mountain belts in western Kyrgyzstan from a temporary local seismic network. A total of 210 crustal earthquakes with hypocentral depths shallower than 25 km were observed during a 12-month period in 2009/2010. The hypocenter distribution indicates a complex net of seismically active structures. The seismicity derived in this study is mainly concentrated at the edges of the Fergana basin, whereas the observed rate of seismicity within the basin is low. The seismicity at the dominant tectonic feature of the region, the Talas-Fergana fault, is likewise low, so the fault seems to be inactive or locked. To estimate the uncertainties of earthquake locations derived in this study, a strong explosion with known origin time and location is used as a ground truth calibration event which suggests a horizontal and vertical accuracy of about 1 km for our relocations. We derived 35 focal mechanisms using first motion polarities and retrieved a set of nine moment tensor solutions for earthquakes with moment magnitude (Mw) ranging from 3.3 to 4.9 by waveform inversion. The solutions reveal both thrust and strike-slip mechanisms compatible with a NW-SE direction of compression for the Fergana region. Two previously unknown tectonic structures in the Fergana region could be identified, both featuring strike-slip kinematics. The combined analysis of the results derived in this study allowed a detailed insight into the currently active tectonic structures and their kinematics where little information had previously been available

Geometric models of the aortic and pulmonary roots: suggestions for the Ross procedure

Objective: To discuss geometric factors, which may influence long-term results relating to homograft competence following the Ross procedure, we describe the 3D morphology of the pulmonary and aortic roots. Materials: Measurements were made on 25 human aortic and pulmonary roots. Inter-commissural distances and the heights of the sinuses were measured. For geometrical reconstruction the three commissures and their vertical projections at the root base were used as reference points. Results: In the pulmonary root, the three inter-commissural distances were of similar dimensions (17.9±1.6mm, 17.5±1.4mm and 18.6±1.5mm). In the aortic root, the right inter-commissural distance was greatest (18.8±1.9mm), followed by the non-coronary (17.4±2.0mm) and left coronary sinus commissures (15.2±1.9mm). The mean height of the left pulmonary sinus was greatest (20±1.7mm) followed by the anterior (17.5±1.4mm) and right pulmonary sinus (18±1.66mm). In the aortic root, the height of the right coronary sinus was the greatest (19.4±1.9mm) followed by the heights of the non-coronary (17.7±1.8mm) and left coronary sinus (17.4±1.4mm). Measured differences between parameters determine the tilt angle and direction of the root vector. The tilt angle in the pulmonary root averaged 16.26°, respectively; for the aortic roots, it was 5.47°. Conclusions: Herein we suggest that the left pulmonary sinus is best implanted in the position of the right coronary sinus, the anterior pulmonary in the position of the non-coronary sinus and the right pulmonary sinus in the position of the left coronary sinus. In this way, the direction of the pulmonary root vector will be parallel to that of the aortic root vecto

Incidence and pathophysiology of atrioventricular block following mitral valve replacement and ring annuloplasty

Background: In this retrospective study we evaluate the causative mechanisms underlying postoperative atrioventricular block (AVB) following mitral valve replacement and mitral valve annuloplasty. Methods: Between January 1990 and December 2003, 391 patients underwent mitral valve replacement or ring annuloplasty and quadrangular resection. Exclusion criteria were preoperative AV block, two or three valvular procedures, reoperations and procedures combined with coronary artery bypass grafting. The presence of the postoperative AVB was compared with preoperative and intraoperative variables. On 55 post-mortem specimens the relationship between the AV node, AV node artery and mitral valve annulus was investigated. Results: The mean age was 59±14 years and 44% of patients were female. Postoperatively AVB occurred in 92 (23.5%) patients. AVB III was found in 17 (4%) patents, in whom a pacemaker was implanted within median interval of 4 days. Second degree AVB occurred and first degree AVB in five (1.3%) and in 70 (18%) patients respectively. In dry dissected human hearts in 23% of investigated cases the AV node artery was discovered to run close to the annulus of the mitral valve. Conclusions: Data collected in this study showed that, sotalol and amiodarone as well as a prolonged cross-clamp time may slightly influence the 23% incidence of postoperative AVB. The morphological investigation showed that the AV node artery runs in close proximity to the annulus in 23% of cases. We speculate that damage of the AV node artery may play a role in development of AV

Advantages of subclavian artery perfusion for repair of acute type A dissection

Objective: Arterial perfusion through the right subclavian artery is proposed to avoid intraoperative malperfusion during repair of acute type A dissection. This study evaluated the clinical and neurological outcome of patients undergoing surgery of acute aortic type A dissection following subclavian arterial cannulation compared to femoral artery approach. Methods: From 1/97 to 1/03, 122 consecutive patients underwent surgery for acute type A aortic dissection. Subclavian cannulation was performed in 62 versus femoral cannulation in 60 patients. Clinical characteristics in both groups were similar. Mean age was 61 years (SD±14 years, 72% male) and mean follow-up was 3 years (±2 years). Patient outcome was assessed as the prevalence of clinical complications, especially neurological deficits, mortality at 30 days, perioperative morbidity and time of body temperature cooling and analyzed by nominal logistic regression analysis for odds ratio calculation. Results: Arterial subclavian cannulation was successfully performed without any occurrence of malperfusion in all cases. Patients undergoing subclavian cannulation showed an odds ratio of 1.98 (95% CI 1.15-3.51; P=0.0057) for an improved neurological outcome compared to patients undergoing femoral cannulation. Re-exploration rate for postoperative bleeding was significantly reduced in the subclavian group (P<0.0001), as well as occurrence of myocardial infarction (P<0.0001) and duration for body temperature cooling (P=0.004). The 30-day mortality of patients with femoral cannulation was significantly higher compared to patients with subclavian artery cannulation (24 versus 8%; P=0.0179). Conclusions: Arterial perfusion through the right subclavian artery provides an excellent approach for repair of acute type A dissection with optimized arterial perfusion body perfusion and allows for antegrade cerebral perfusion during circulatory arrest. The technique is safe and results in a significantly improved clinical and especially neurological outcom

Modified ultrafiltration lowers adhesion molecule and cytokine levels after cardiopulmonary bypass without clinical relevance in adults

Objective: Cardiac surgery with cardiopulmonary bypass (CPB) results in expression of cytokines and adhesion molecules (AM) with subsequent inflammatory response. The purpose of the study was to evaluate the clinical impact of modified ultrafiltration (MUF) and its efficacy in reducing cytokines and AM following coronary artery bypass grafting (CABG) in adults. Methods: A prospective randomized study of 97 patients undergoing elective CABG was designed. Fifty patients were operated on using normothermic and 47 patients using hypothermic CPB. The normothermic group was subdivided into a group with modified ultrafiltration (n=30) and a group without MUF (n=20). In the hypothermic group 30 patients received MUF compared to 17 patients serving as controls. MUF was instituted after CPB for 15 min through the arterial and venous bypass circuit lines. Cytokines (IL-6, IL-8, TNF-α, IL-2R) and adhesion molecules (sE-selectin, sICAM-1) were measured preoperatively, pre-MUF, in the ultrafiltrate, 24 h, 48 h and 6 days after surgery by chemiluminescent enzyme immunometric assay or enzyme-linked immunosorbent assay (ELISA). Clinical parameters were collected prospectively until discharge. Results: In all patients AM and cytokines were significantly elevated after normothermic and hypothemic CPB. AM and cytokines were significantly higher in hypothermia compared to normothermia. In hypothermic CPB sE-selectin was decreased after 24 h by 37% (P<0.0063) and by 40% (P<0.0027) after 48 h postoperatively. ICAM-1 was reduced by 43% (P<0.0001) after 24 h and by 60% (P<0.0001) after 6 days. Similar results were seen in cytokines with reduction up to 60% after 24 h. Changes after 48 h were noticeable but not significant. Reduction of AM and cytokines after normothermic CPB was minimal. Neither in normothermia, nor in hypothermia has sIL-2R been effectively removed from the circulation. There were no significant differences in the clinical variables between the patients with or without MUF. Conclusion: AM and cytokines are significantly elevated after hypothermic CPB compared to normothermic CPB. MUF led to a significant reduction in cytokine and AM levels after hypothermic CPB, except for IL-2R. MUF showed minimal effect in normothermia. We conclude that MUF is an efficient way to remove cytokines and AM. However, we were unable to demonstrate any significant impact of MUF in outcome of adults after elective CAB

Root-root interactions: extending our perspective to be more inclusive of the range of theories in ecology and agriculture using in-vivo analyses

Background There is a large body of literature on competitive interactions among plants, but many studies have only focused on above-ground interactions and little is known about root-root dynamics between interacting plants. The perspective on possible mechanisms that explain the outcome of root-root interactions has recently been extended to include non-resource-driven mechanisms (as well as resource-driven mechanisms) of root competition and positive interactions such as facilitation. These approaches have often suffered from being static, partly due to the lack of appropriate methodologies for in-situ non-destructive root characterization. Scope Recent studies show that interactive effects of plant neighbourhood interactions follow non-linear and non-additive paths that are hard to explain. Common outcomes such as accumulation of roots mainly in the topsoil cannot be explained solely by competition theory but require a more inclusive theoretical, as well as an improved methodological framework. This will include the question of whether we can apply the same conceptual framework to crop versus natural species. Conclusions The development of non-invasive methods to dynamically study root-root interactions in vivo will provide the necessary tools to study a more inclusive conceptual framework for root-root interactions. By following the dynamics of root-root interactions through time in a whole range of scenarios and systems, using a wide variety of non-invasive methods, (such as fluorescent protein which now allows us to separately identify the roots of several individuals within soil), we will be much better equipped to answer some of the key questions in root physiology, ecology and agronom