Effect of Ethanol Extract of Sugar Apple (Annona squamosa L.) Stem Bark on Rat SGPT and SGOT

This study aims to compare hepatoprotector potency of the ethanol extract of sugar apple (A. squamosa) stem bark (EESSB) against the enzyme activity of SGOT and SGPT in rats induced by toxic doses of paracetamol. The method used in this study is Completely Randomized (CRD) consisting of 6 treatment groups and 3 replications. The parameters that are being observed in this study are phytochemical, antioxidant, clinical symptoms, macroscopic, SGOT and SGPT rats. Data were analyzed using Analysis of Variance (ANOVA) with a significant level of 5% with the Duncan test as a follow-up test. The clinical and macroscopic symptoms of rat liver including changes in color, surface structure and consistency as well as relative organ weight. Data were analyzed using ANOVA significant level 5% and Duncan's test as a follow-up test.  Extracts contains alkaloid, steroid and phenolic compounds. The results showed that there was a significant difference (P <0.05) in the levels of SGOT and SGPT after giving ethanol extract of stem bark A.squamosa (EESSB). Based on the results of the study, it was concluded that all EESSB doses had the potential to reduce the levels of AST and ALT in paracetamol induced rats. The results showed that there was a significant difference (P <0.05) in the macroscopic changes in the liver based on changes in color and degree of liver damage. Relative organ weight had no significant effect (P> 0.05). The ethanol extract of  A. squamosa (EESSB) stem bark has the potential to be a hepatoprotector, however a dose of 150 mg/kg BW is a more effective dose as a hepatoprotector against paracetamol

PENGARUH PEMBERIAN EKSTRAK ETANOL KULIT BATANG SIRSAK (Annona muricata L.) DAN SRIKAYA (Annona squamosa L.) TERHADAP HATI TIKUS (Rattus novergicus L.) GALUR WISTAR

Penelitian ini bertujuan mengetahui pengaruh ekstrak etanol kulit batang Annona muricata dan Annona squamosa terhadap perubahan struktur makroskopis hati, serta gejala klinis pada tikus yang diinduksi parasetamol. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) yang terdiri dari 9 perlakuan dan 3 ulangan. Sebanyak 27 tikus diinduksi hepatotoksisitas dengan parasetamol sebanyak 1350 mg/kgBB pada hari ke-7, kecuali kontrol normal (K0) yang diberi akuades. Hewan uji juga mendapat perlakuan pemberian akuades pada kontrol negatif (K-), Hepa-Q sebanyak 11,34 mg/kgBB kontrol positif (K+), Ekstrak Etanol Kulit Batang Annona muricata dosis 150 mg/kgBB (P1), 300 mg/kgBB (P2) dan 600 mg/kgBB (P3). Ekstrak Etanol Kulit Batang Annona squamosa dosis 150 mg/kgBB (P4), 300 mg/kgBB (P5) dan 600 mg/kgBB (P6) selama 14 hari. Parameter yang diamati adalah Gejala klinis dan makroskopis hati tikus meliputi perubahan warna. Data dianalisis menggunakan Analysis of Variance (ANOVA) taraf signifikan 5% dan uji Duncan sebagai uji lanjut. Hasil penelitian menunjukkan terdapat perbedaan yang nyata (P<0,05) pada perubahan makroskopis hati berdasarkan perubahan warna dan derajat kerusakan hati. Ekstrak etanol kulit batang kedua spesies tumbuhan tersebut berpotensi sebagai hepatoprotektor dimana dosis 150 mg/kgBB adalah dosis yang efektif sebagai bahan hepatoprotektor terhadap parasetamol