Additional file 3: Figure S2. of IL-1β mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation

Increased immune cells and total protein in BALF in both WT and IL-1β−/− groups 24 h after the last HDM challenge alone. Total protein in BALF (a), total cells from BALF (b) and total neutrophil count in BALF (c). Data are presented as mean ± SEM, n = 4–7 mice in each group. ## = p < 0.01 compared to respective HDM/Saline control, and # = p < 0.05 compared to respective HDM/Saline control. (TIFF 941 kb

Additional file 2: Table S1. of IL-1β mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation

Commercially available primer sequences for genes analysed with RT-qPCR. (DOCX 13 kb

Additional file 4: Figure S3. of IL-1β mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation

Increased immune cell count and chemokine expression at exacerbation, although in both WT and IL-1β−/− mice. Total eosinophil count (a), macrophage count (b) and lymphocyte count (c) from BALF at exacerbation. Chemokine mRNA expression of CCL11 (d) and CCL2 (e) from lung tissue homogenates were analysed with RT-qPCR. The relative gene expression was related to the reference gene 18S and normalised to control. Data are presented as mean ± SEM, n = 4–8 mice in each group. ## = p < 0.01 compared to respective HDM/Saline control. (TIFF 1200 kb