Increased immune cells and total protein in BALF in both WT and IL-1β−/− groups 24 h after the last HDM challenge alone. Total protein in BALF (a), total cells from BALF (b) and total neutrophil count in BALF (c). Data are presented as mean ± SEM, n = 4–7 mice in each group. ## = p < 0.01 compared to respective HDM/Saline control, and # = p < 0.05 compared to respective HDM/Saline control. (TIFF 941 kb