Flowchart of the study design.

<p>Flowchart of the study design.</p

Significant expression results for hexadecanedioate in adipocytes.

<p>Significant expression results for hexadecanedioate in adipocytes.</p

Manhattan plot showing genome-wide P values from association analysis of hexadecanedioate in the TwinsUK-KORA meta-analysis.

<p>The y axis shows the −log10 P values of SNPs, and the x axis shows their chromosomal positions.</p

Association between SNPs in the <i>SLCO1B1</i> gene and <i>CYP4</i> cluster and hexadecanedioate levels in the meta analysis carried out by Shin et al (2014)[7], in the extremes of BP carried out by Padmanabhan et al (2010)[11], in the ICBP consortium GWAS(2011)[10] and with risk of statin associated myopathy as reported (SEARCH collaborative group 2008)[14].

<p>Association between SNPs in the <i>SLCO1B1</i> gene and <i>CYP4</i> cluster and hexadecanedioate levels in the meta analysis carried out by Shin et al (2014)[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0175479#pone.0175479.ref007" target="_blank">7</a>], in the extremes of BP carried out by Padmanabhan et al (2010)[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0175479#pone.0175479.ref011" target="_blank">11</a>], in the ICBP consortium GWAS(2011)[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0175479#pone.0175479.ref010" target="_blank">10</a>] and with risk of statin associated myopathy as reported (SEARCH collaborative group 2008)[<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0175479#pone.0175479.ref014" target="_blank">14</a>].</p

Flowchart of the study design.

<p>Flowchart of the study design.</p

Alcohol intake and BP stratified by hexadecanedioate levels adjusting for age, age², BMI and family relatedness.

<p>Alcohol intake and BP stratified by hexadecanedioate levels adjusting for age, age², BMI and family relatedness.</p

Association plot of the genomic region around (i) SLCO1B1, (ii) CYP4B1 and (iii)ADH1B showing both type and imputed SNPs with location of genes and recombination rate.

<p><i>P</i> values of SNPs are plotted (as −log₁₀ <i>P</i>) against their physical position on the chromosomes (NCBI Build 36). Estimated recombination rates from the HapMap CEU population show the local LD structure. The color of each SNP indicates linkage disequilibrium with the index SNP (rs4149056 or rs6663731 or rs1693457 respectively) based on pairwise <i>r</i>² values from HapMap CEU data.</p

The effect of comorbidity on the occurrence of depression and/or angina in GS:SFHS overall.

<p>The effect of comorbidity on the occurrence of depression and/or angina in GS:SFHS overall.</p

Co-occurrence of chronic pain, major depressive disorder and/or angina in GS:SFHS overall.

<p>Co-occurrence of chronic pain, major depressive disorder and/or angina in GS:SFHS overall.</p

Phenotypic correlations (r) with [95% confidence intervals] between observed variables in TwinsUK (n = 2,902).

<p>Phenotypic correlations (r) with [95% confidence intervals] between observed variables in TwinsUK (n = 2,902).</p