145 research outputs found
Normative data of the start in the 50 m events at the 2021 LEN European Championships and understanding its relationship with the final race
This study aimed to: (i) present normative data of the variables related to the start in
the four swim strokes by tier and sex, and; (ii) understand the relationship between the 15th
meter mark time and the final race time of the male and female swimmers competing in the four
50 m events at the 2021 European Championships. Participants were all male and female
swimmers who competed in the 50 m events at the 2021 LEN European Championships held in
Budapest. The official race times and block times were retrieved from the official competition
website. All starting variables were analyzed in a dedicated software for race analysis. The 15th
meter mark time was used as the start main outcome. For all events by sex, the 15th meter mark
time was the variable presenting the highest and largest tier effect (p < 0.001) besides the final
race time. Overall, despite the swim stroke, the variables related to the underwater phase were
also responsible for the significant tier effect (p < 0.001). The 15th meter mark time presented a
high to very-high relationship with the final race time in all four swim strokes. This relationship
was stronger in freestyle (both sexes). That is, swimmers who achieve the 15th meter mark sooner
are more likely to deliver better performances. Coaches must be aware that the underwater
phase plays a key-role on the swimmers’ (both sexes) start performance. Nonetheless, different
strategies can be used based on the swimmers’ strength and weaknesses. Moreover, the start
performance in all four swim strokes and in both sexes can strongly predict the final race time.This research was supported by national funds (FCT - Portuguese Foundation for Science
and Technology) under the project UIDB/DTP/04045/2020info:eu-repo/semantics/publishedVersio
Identification of the glycosylphosphatidylinositol-specific phospholipase A2 (GPI-PLA2) that mediates GPI fatty acid remodeling in Trypanosoma brucei
The biosynthesis of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) in the parasitic protozoan Trypanosoma brucei involves fatty acid remodeling of the GPI precursor molecules before they are transferred to protein in the endoplasmic reticulum. The genes encoding the requisite phospholipase A2 and A1 activities for this remodeling have thus far been elusive. Here, we identify a gene, Tb927.7.6110, that encodes a protein that is both necessary and sufficient for GPI-phospholipase A2 (GPI-PLA2) activity in the procyclic form of the parasite. The predicted protein product belongs to the alkaline ceramidase, PAQR receptor, Per1, SID-1, and TMEM8 (CREST) superfamily of transmembrane hydrolase proteins and shows sequence similarity to Post-GPI-Attachment to Protein 6 (PGAP6), a GPI-PLA2 that acts after transfer of GPI precursors to protein in mammalian cells. We show the trypanosome Tb927.7.6110 GPI-PLA2 gene resides in a locus with two closely related genes Tb927.7.6150 and Tb927.7.6170, one of which (Tb927.7.6150) most likely encodes a catalytically inactive protein. The absence of GPI-PLA2 in the null mutant procyclic cells not only affected fatty acid remodeling but also reduced GPI anchor sidechain size on mature GPI-anchored procyclin glycoproteins. This reduction in GPI anchor sidechain size was reversed upon the re-addition of Tb927.7.6110 and of Tb927.7.6170, despite the latter not encoding GPI precursor GPI-PLA2 activity. Taken together, we conclude that Tb927.7.6110 encodes the GPI-PLA2 of GPI precursor fatty acid remodeling and that more work is required to assess the roles and essentiality of Tb927.7.6170 and the presumably enzymatically inactive Tb927.7.6150
Nomenclatural and taxonomic review of Passifloraceae species illustrated and described by Vellozo in Flora Fluminensis
Relação entre variáveis na regulação do consumo de vacas Holandês × Zebu em lactação sob pastejo em capim-elefante
Hemograma, bioquímica sérica e histologia da biópsia hepática de bovinos após administração de polpa cítrica
Taxa de concepção de cabras inseminadas com sêmen caprino resfriado a 5ºC, por 12 ou 24 horas, em meio diluidor à base de gema de ovo
Glycosaminoglycans affect the interaction of human plasma kallikrein with plasminogen, factor XII and inhibitors
Avaliação da qualidade microbiológica de bebida láctea e creme de leite UAT por ATP-Bioluminescência
Animal performance and carcass characteristics of Nellore young bulls fed coated or uncoated urea slaughtered at different weights
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