Normative data of the start in the 50 m events at the 2021 LEN European Championships and understanding its relationship with the final race

This study aimed to: (i) present normative data of the variables related to the start in the four swim strokes by tier and sex, and; (ii) understand the relationship between the 15th meter mark time and the final race time of the male and female swimmers competing in the four 50 m events at the 2021 European Championships. Participants were all male and female swimmers who competed in the 50 m events at the 2021 LEN European Championships held in Budapest. The official race times and block times were retrieved from the official competition website. All starting variables were analyzed in a dedicated software for race analysis. The 15th meter mark time was used as the start main outcome. For all events by sex, the 15th meter mark time was the variable presenting the highest and largest tier effect (p < 0.001) besides the final race time. Overall, despite the swim stroke, the variables related to the underwater phase were also responsible for the significant tier effect (p < 0.001). The 15th meter mark time presented a high to very-high relationship with the final race time in all four swim strokes. This relationship was stronger in freestyle (both sexes). That is, swimmers who achieve the 15th meter mark sooner are more likely to deliver better performances. Coaches must be aware that the underwater phase plays a key-role on the swimmers’ (both sexes) start performance. Nonetheless, different strategies can be used based on the swimmers’ strength and weaknesses. Moreover, the start performance in all four swim strokes and in both sexes can strongly predict the final race time.This research was supported by national funds (FCT - Portuguese Foundation for Science and Technology) under the project UIDB/DTP/04045/2020info:eu-repo/semantics/publishedVersio

Identification of the glycosylphosphatidylinositol-specific phospholipase A2 (GPI-PLA2) that mediates GPI fatty acid remodeling in Trypanosoma brucei

The biosynthesis of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) in the parasitic protozoan Trypanosoma brucei involves fatty acid remodeling of the GPI precursor molecules before they are transferred to protein in the endoplasmic reticulum. The genes encoding the requisite phospholipase A2 and A1 activities for this remodeling have thus far been elusive. Here, we identify a gene, Tb927.7.6110, that encodes a protein that is both necessary and sufficient for GPI-phospholipase A2 (GPI-PLA2) activity in the procyclic form of the parasite. The predicted protein product belongs to the alkaline ceramidase, PAQR receptor, Per1, SID-1, and TMEM8 (CREST) superfamily of transmembrane hydrolase proteins and shows sequence similarity to Post-GPI-Attachment to Protein 6 (PGAP6), a GPI-PLA2 that acts after transfer of GPI precursors to protein in mammalian cells. We show the trypanosome Tb927.7.6110 GPI-PLA2 gene resides in a locus with two closely related genes Tb927.7.6150 and Tb927.7.6170, one of which (Tb927.7.6150) most likely encodes a catalytically inactive protein. The absence of GPI-PLA2 in the null mutant procyclic cells not only affected fatty acid remodeling but also reduced GPI anchor sidechain size on mature GPI-anchored procyclin glycoproteins. This reduction in GPI anchor sidechain size was reversed upon the re-addition of Tb927.7.6110 and of Tb927.7.6170, despite the latter not encoding GPI precursor GPI-PLA2 activity. Taken together, we conclude that Tb927.7.6110 encodes the GPI-PLA2 of GPI precursor fatty acid remodeling and that more work is required to assess the roles and essentiality of Tb927.7.6170 and the presumably enzymatically inactive Tb927.7.6150