Recueil, traçabilité et restitution des données territoriales du programme ESPON

International audienceThe ESPON M4D Multi-Dimensional Database Design and Development Project entails integrating, verifying and presenting the territorial data produced by ESPON Applied Research Projects. The main challenges that the project faced were managing, standardising and coordinating a wealth of diverse data, ensuring data quality, traceability and creating display tools for territorial data. Implementing specialised methods and tools has provided solutions and opportunities for further analysis. It is clear that centralising the data management and processing helps a wider audience to access this data.Le projet ESPON M4D Multi Dimensional Database Design and Development consiste à intégrer, vérifier et restituer les données territoriales produites par les projets de recherche appliquée du programme européen ESPON. Les principaux enjeux de ce projet consistent à gérer la profusion de données hétérogènes, les normaliser et les harmoniser, évaluer leur qualité, assurer leur traçabilité, créer des outils de suivi et de restitution de ces données. La mise en place de tels méthodes et outils adaptés apportent quelques solutions et pistes de réflexion. Force est de constater que la centralisation de la gestion et du traitement de ces données aide à leur restitution à un très large public

Recueil, traçabilité et restitution des données territoriales du programme ESPON

International audienceThe ESPON M4D Multi-Dimensional Database Design and Development Project entails integrating, verifying and presenting the territorial data produced by ESPON Applied Research Projects. The main challenges that the project faced were managing, standardising and coordinating a wealth of diverse data, ensuring data quality, traceability and creating display tools for territorial data. Implementing specialised methods and tools has provided solutions and opportunities for further analysis. It is clear that centralising the data management and processing helps a wider audience to access this data.Le projet ESPON M4D Multi Dimensional Database Design and Development consiste à intégrer, vérifier et restituer les données territoriales produites par les projets de recherche appliquée du programme européen ESPON. Les principaux enjeux de ce projet consistent à gérer la profusion de données hétérogènes, les normaliser et les harmoniser, évaluer leur qualité, assurer leur traçabilité, créer des outils de suivi et de restitution de ces données. La mise en place de tels méthodes et outils adaptés apportent quelques solutions et pistes de réflexion. Force est de constater que la centralisation de la gestion et du traitement de ces données aide à leur restitution à un très large public

Prospective pathways to depressive symptoms and disordered eating in adolescence: A 7-year longitudinal cohort study

Eating pathology and depressive symptoms increase during adolescence, yet predictive pathways remain predominantly unexplored, despite their implications for prevention. The present study aimed to identify shared risk factors for eating pathology and depressive symptoms by evaluating an adapted Dual-Pathway Model of disordered eating, which postulated that higher BMI would predict disordered eating and depressive symptoms via pathways between body dissatisfaction, later BMI, depressive symptoms, and visible indicators of puberty (breast development for girls, height for boys). The participants were 8,915 children (49% girls) from the Avon Longitudinal Study of Parents and Children, a population-based cohort study of British children, who were assessed at different intervals between the age of 7 to 14 years. Path analyses revealed that, for girls, childhood BMI exerted indirect effects on disordered eating via body dissatisfaction, depressive symptoms, and more advanced breast development, with indirect pathways identified to depressive symptoms via earlier depressive symptoms and more advanced breast development. For boys, childhood BMI had indirect effects on disordered eating via later BMI and body dissatisfaction, while only earlier depressive symptoms were found to have an independent and direct effect on adolescent depressive symptoms. This study reveals shared and independent risk factors for eating pathology and depressive symptoms in adolescence and suggests targets for preventative interventions, including higher BMI, body dissatisfaction, and depressive symptoms, in addition to advanced breast development, for girls

STedi : une infrastructure logicielle pour renforcer la qualité des données territoriales statistiques

International audienceSpatial information must now meet standards to ensure data systems interoperability. While ISO standards, recommended by the INSPIRE directive in force, are adapted to environmental data, still many adjustments are necessary to adapt standards to territorial statistical data. Therefore, the INSPIRE data model is extended as part of the " ESPON Database 2013 " project. This article presents the " STedi " software infrastructure and its components based on the INSPIRE extended model proposed. This operational infrastructure enables the management of a data flow as a whole: from the provider to the user, from the acquisition to the retrieval of data and metadata. The infrastructure provides the oversight of data and metadata collected through software components such as the " Checking tool " , which is in charge of datasets quality process organisation. The modular infrastructure is compliant with standards and is transposable to multiple domains. MOTS-CLES : INSPIRE, modèle de données, qualité des données, infrastructure logicielle.L'information à référence spatiale doit aujourd'hui respecter des normes pour garantir l'interopérabilité des systèmes de données. Si les normes ISO recommandées par la directive INSPIRE en vigueur sont adaptées aux données environnementales, mettre en oeuvre la directive pour des données statistiques territoriales nécessite des adaptations. Le modèle de données INSPIRE est alors étendu pour la représentation de ces données, dans le cadre du projet « ESPON Database 2013 ». Cet article présente l'infrastructure logicielle « STedi » et ses composants reposant sur le modèle INSPIRE étendu proposé. Cette infrastructure opérationnelle permet la gestion d'un flux de données dans son ensemble : du fournisseur à l'utilisateur, de l'acquisition à la restitution des données et métadonnées. L'infrastructure veille au contrôle des données et métadonnées recueillies par le biais de briques logicielles telles que le « Checking tool », chargée d'organiser le processus de vérification de la qualité des jeux de données. Le caractère modulaire et le respect de standards font de l'infrastructure un système transposable à de multiples domaines

STedi : une infrastructure logicielle pour renforcer la qualité des données territoriales statistiques

International audienceSpatial information must now meet standards to ensure data systems interoperability. While ISO standards, recommended by the INSPIRE directive in force, are adapted to environmental data, still many adjustments are necessary to adapt standards to territorial statistical data. Therefore, the INSPIRE data model is extended as part of the " ESPON Database 2013 " project. This article presents the " STedi " software infrastructure and its components based on the INSPIRE extended model proposed. This operational infrastructure enables the management of a data flow as a whole: from the provider to the user, from the acquisition to the retrieval of data and metadata. The infrastructure provides the oversight of data and metadata collected through software components such as the " Checking tool " , which is in charge of datasets quality process organisation. The modular infrastructure is compliant with standards and is transposable to multiple domains. MOTS-CLES : INSPIRE, modèle de données, qualité des données, infrastructure logicielle.L'information à référence spatiale doit aujourd'hui respecter des normes pour garantir l'interopérabilité des systèmes de données. Si les normes ISO recommandées par la directive INSPIRE en vigueur sont adaptées aux données environnementales, mettre en oeuvre la directive pour des données statistiques territoriales nécessite des adaptations. Le modèle de données INSPIRE est alors étendu pour la représentation de ces données, dans le cadre du projet « ESPON Database 2013 ». Cet article présente l'infrastructure logicielle « STedi » et ses composants reposant sur le modèle INSPIRE étendu proposé. Cette infrastructure opérationnelle permet la gestion d'un flux de données dans son ensemble : du fournisseur à l'utilisateur, de l'acquisition à la restitution des données et métadonnées. L'infrastructure veille au contrôle des données et métadonnées recueillies par le biais de briques logicielles telles que le « Checking tool », chargée d'organiser le processus de vérification de la qualité des jeux de données. Le caractère modulaire et le respect de standards font de l'infrastructure un système transposable à de multiples domaines

Recueil, traçabilité et restitution des données territoriales du programme ESPON

Le projet ESPON M4D consiste à collecter, vérifier, intégrer et restituer les données territoriales produites par les projets du programme de recherche appliquée ESPON. Les principaux enjeux de ce projet consistent en premier lieu à gérer la profusion de données hétérogènes, les normaliser et les harmoniser, évaluer leur qualité et assurer leur suivi. Ensuite, de créer des outils de suivi et de restitution de ces données. La mise en place de ces méthodes et outils adaptés apporte quelques solutions et pistes de réflexion pour le lecteur s’intéressant à la reproduction de cette expérience de projet dans un environnement similaire (montage d’observatoire, coordination d’acteurs producteurs de données multiples, etc.).The ESPON M4D Project entails gathering, verifying, integrating and presenting the territorial data produced by ESPON Applied Research Projects. The main challenges that the project faced were firstly managing, standardising and coordinating a wealth of diverse data, ensuring data quality and traceability. The second hurdle was the creation of tracking and display tools for territorial data. Implementing specialised methods and tools has provided solutions and opportunities for further analysis in the same field of applied research (data center creation, coordination of several data providers).Con el proyecto ESPON M4D se ha recogido, verificado, integrado y restituido la información territorial de los proyectos de investigación aplicada de ESPON. Sus principales retos consisten en poder gestionar datos muy heterogéneos, normalizarlos, armonizarlos, evaluar su calidad y asegurar su continuidad. Este método de trabajo y sus herramientas de trabajo aportan innovaciones y procedimientos de trabajo que pueden servir al lector para replicarlos en entornos similares (observatorios territoriales, coordinación de proveedores de información, etc.)

Background

PROCEEDINGS A rich internet application for remote visualization and collaborative annotation of digital slides in histology and cytolog

Early immune response following Salmonella enterica subspecies enterica serovar Typhimurium infection in porcine jejunal gut loops

Salmonella enterica subspecies enterica serovar Typhimurium, commonly called S. Typhimurium, can cause intestinal infections in humans and various animal species such as swine. To analyze the host response to Salmonella infection in the pig we used an in vivo gut loop model, which allows the analysis of multiple immune responses within the same animal. Four jejunal gut-loops were each inoculated with 3×108 cfu of S. Typhimurium in 3 one-month-old piglets and mRNA expressions of various cytokines, chemokines, transcription factors, antimicrobial peptides, toll like and chemokine receptors were assessed by quantitative real-time PCR in the Peyer’s patch and the gut wall after 24 h. Several genes such as the newly cloned CCRL1/CCX-CKR were assessed for the first time in the pig at the mRNA level. Pro-inflammatory and T-helper type-1 (Th1) cytokine mRNA were expressed at higher levels in infected compared to non-infected control loops. Similarly, some B cell activation genes, NOD2 and toll like receptor 2 and 4 transcripts were more expressed in both tissues while TLR5 mRNA was down-regulated. Interestingly, CCL25 mRNA expression as well as the mRNA expressions of its receptors CCR9 and CCRL1 were decreased both in the Peyer’s patch and gut wall suggesting a potential Salmonella strategy to reduce lymphocyte homing to the intestine. In conclusion, these results provide insight into the porcine innate mucosal immune response to infection with entero-invasive microorganisms such as S. Typhimurium. In the future, this knowledge should help in the development of improved prophylactic and therapeutic approaches against porcine intestinal S. Typhimurium infections

Induction of α1-tubulin gene expression during development and regeneration of the fish central nervous system

The α1- and α2-tubulin encoding genes were cloned from a goldfish genomic DNA library. α1- and α2-tubulin RNA expression was examined in developing and adult retinas. These studies demonstrated increased α1-tubulin RNA in presumptive ganglion cells that grow axons early in retinal development and in adult retinal ganglion cells whose optic axons had been damaged. The α2-tubulin RNA was undetectable in developing retina and constitutively expressed in adult retinal ganglion cells regardless of optic nerve crush. To determine if these changes in α1-tubulin RNA reflected changes in α1-tubulin promoter activity, we introduced into zebrafish embryos and adult goldfish retinal explants expression vectors harboring the α1-tubulin gene's promoter. These studies showed that the α1-tubulin promoter confers a developmentally regulated, neuron-restricted pattern of reporter gene expression in vivo and its activity is increased in adult retinal neurons induced to regenerate their axons. Promoter deletions defined regions of α1-tubulin DNA necessary for this pattern of expression. These results suggest that DNA sequences necessary for α1-tubulin gene induction during central nervous system development and regeneration are contained within the α1-tubulin gene's 5′-flanking DNA and that this promoter will be useful for identifying these elements and their DNA binding proteins. © 1998 John Wiley & Sons, Inc. J Neurobiol 37: 429–440, 1998Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34478/1/8_ftp.pd

Cellular Immune Responses Induced with Dose-Sparing Intradermal Administration of HIV Vaccine to HIV-Uninfected Volunteers in the ANRS VAC16 Trial

The objective was to compare the safety and cellular immunogenicity of intradermal versus intramuscular immunization with an HIV-lipopeptide candidate vaccine (LIPO-4) in healthy volunteers.A randomized, open-label trial with 24 weeks of follow-up was conducted in France at six HIV-vaccine trial sites. Sixty-eight healthy 21- to 55-year-old HIV-uninfected subjects were randomized to receive the LIPO-4 vaccine (four HIV lipopeptides linked to a T-helper-stimulating epitope of tetanus-toxin protein) at weeks 0, 4 and 12, either intradermally (0.1 ml, 100 microg of each peptide) or intramuscularly (0.5 ml, 500 microg of each peptide). Comparative safety of both routes was evaluated. CD8+ T-cell immune responses to HIV epitopes (ELISpot interferon-gamma assay) and tetanus toxin-specific CD4+ T-cell responses (lymphoproliferation) were assessed at baseline, two weeks after each injection, and at week 24.No severe, serious or life-threatening adverse events were observed. Local pain was significantly more frequent after intramuscular injection, but local inflammatory reactions were more frequent after intradermal immunization. At weeks 2, 6, 14 and 24, the respective cumulative percentages of induced CD8+ T-cell responses to at least one HIV peptide were 9, 33, 39 and 52 (intradermal group) or 14, 20, 26 and 37 (intramuscular group), and induced tetanus toxin-specific CD4+ T-cell responses were 6, 27, 33 and 39 (intradermal), or 9, 46, 54 and 63 (intramuscular). In conclusion, intradermal LIPO-4 immunization was well tolerated, required one-fifth of the intramuscular dose, and induced similar HIV-specific CD8+ T-cell responses. Moreover, the immunization route influenced which antigen-specific T-cells (CD4+ or CD8+) were induced.ClinicalTrials.gov NCT00121121