User Needs for Electronic Document Management in Public Administration: A Study of Two Cases

Development of new practices for managing documents in their electronic form in public administration requires extensive knowledge about the domain, its processes, documents, and the needs of both organisations and individual people. The paper introduces two cases where the needs concerning electronic document management are studied. The first case concerns the creation of the state budget in Finland, and the second one concerns the Finnish participation in EU legislative work. In both of the cases the study revealed many needs related to different aspects of electronic document management: documents, information technology, and work with documents. The study is part of the research related to SGML standardisation in the Finnish Parliament and ministries

Characterization of Highly Mucus-Adherent Non-GMO Derivatives of Lacticaseibacillus rhamnosus GG

Lacticaseibacillus rhamnosusGG is one of the best studied lactic acid bacteria in the context of probiotic effects.L. rhamnosusGG has been shown to prevent diarrhea in children and adults and has been implicated to have mitigating or preventive effects in several disorders connected to microbiota dysbiosis. The probiotic effects are largely attributed to its adhesive heterotrimeric sortase-dependent pili, encoded by thespaCBA-srtC1gene cluster. Indeed, the strain-specific SpaCBA pili have been shown to contribute to adherence, biofilm formation and host signaling. In this work we set out to generate non-GMO derivatives ofL. rhamnosusGG that adhere stronger to mucus compared to the wild-type strain using chemical mutagenesis. We selected 13 derivatives that showed an increased mucus-adherent phenotype. Deep shotgun resequencing of the strains enabled division of the strains into three classes, two of which revealed SNPs (single nucleotide polymorphisms) in thespaAandspaCgenes encoding the shaft and tip adhesive pilins, respectively. Strikingly, the other class derivatives demonstrated less clear genotype - phenotype relationships, illustrating that pili biogenesis and structure is also affected by other processes. Further characterization of the different classes of derivatives was performed by PacBio SMRT sequencing and RNAseq analysis, which resulted in the identification of molecular candidates driving pilin biosynthesis and functionality. In conclusion, we report on the generation and characterization of three classes of strongly adherentL. rhamnosusGG derivatives that show an increase in adhesion to mucus. These are of special interest as they provide a window on processes and genes driving piliation and its control inL. rhamnosusGG and offer a variety of non-GMO derivatives of this key probiotic strain that are applicable in food products.Peer reviewe

Requirements for xml document database systems

The shift from SGML to XML has created new demands for managing structured documents. Many XML documents will be transient representations for the purpose of data exchange between different types of applications, but there will also be a need for effective means to manage persistent XML data as a database. In this paper we explore requirements for an XML database management system. The purpose of the paper is not to suggest a single type of system covering all necessary features. Instead the purpose is to initiate discussion of the requirements arising from document collections, to offer a context in which to evaluate current and future solutions, and to encourage the development of proper models and systems for XML database management. Our discussion addresses issues arising from data modelling, data definition, and data manipulation