High-power gyrotrons for electron cyclotron heating and current drive

In many tokamak and stellarator experiments around the globe that are investigating energy production via controlled thermonuclear fusion, electron cyclotron heating and current drive (ECH&CD) are used for plasma start-up, heating, non-inductive current drive and MHD stability control. ECH will be the first auxiliary heating method used on ITER. Megawatt-class, continuous wave (CW) gyrotrons are employed as high-power millimeter (mm)-wave sources. The present review reports on the worldwide state-of-the-art of high-power gyrotrons. Their successful development during the past years changed ECH from a minor to a major heating method. After a general introduction of the various functions of ECH&CD in fusion physics, especially for ITER, Section 2 will explain the fast-wave gyrotron interaction principle. Section 3 discusses innovations on the components of modern long-pulse fusion gyrotrons (magnetron injection electron gun (MIG), beam tunnel, cavity, quasi-optical output coupler, synthetic diamond output window, single-stage depressed collector) and auxiliary components (superconducting magnets, gyrotron diagnostics, high-power calorimetric dummy loads). Section 4 deals with present megawatt-class gyrotrons for ITER, W7-X, LHD, EAST, KSTAR and JT-60SA, and also includes tubes for moderate pulse length machines as, ASDEX-U, DIII-D, HL-2A, TCV, QUEST and GAMMA-10. In Section 5 the development of future advanced fusion gyrotrons is discussed. These are tubes with higher frequencies for DEMO, multi-frequency (multi-purpose) gyrotrons, stepwise frequency tunable tubes for plasma stabilization, injection-locked and coaxial-cavity multi-megawatt gyrotrons, as well as sub-THz gyrotrons for collective Thomson scattering (CTS). Efficiency enhancement via multi-stage depressed collectors, fast oscillation recovery methods and reliability, availability, maintainability and inspectability (RAMI) will be discussed at the end of this section

Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus

Pleurotus ostreatus is one of the most commercially produced edible mushrooms worldwide. Improved cultivated strains with more useful traits have been obtained using classical breeding, which is laborious and time-consuming. Here, we attempted efficient gene mutagenesis using plasmid-based CRISPR/Cas9 as the first step for non-genetically modified (non-GM) P. ostreatus generation. Plasmids harboring expression cassettes of Cas9 and different single guide RNAs targeting fcy1 and pyrG were individually transferred into fungal protoplasts of the PC9 strain, which generated some strains exhibiting resistance to 5-fluorocytosine and 5-fluoroorotic acid, respectively. Genomic PCR followed by sequencing revealed small insertions/deletions or insertion of a fragment from the plasmid at the target site in some of the drug-resistant strains. The results demonstrated efficient CRISPR/Cas9-assisted genome editing in P. ostreatus, which could contribute to the molecular breeding of non-GM cultivated strains in the future. Furthermore, a mutation in fcy1 via homology-directed repair using this CRISPR/Cas9 system was also efficiently introduced, which could be applied not only for precise gene disruption, but also for insertions leading to heterologous gene expression in this fungus

Efficient genome editing with CRISPR/Cas9 in Pleurotus ostreatus

Pleurotus ostreatus is one of the most commercially produced edible mushrooms worldwide. Improved cultivated strains with more useful traits have been obtained using classical breeding, which is laborious and time-consuming. Here, we attempted efficient gene mutagenesis using plasmid-based CRISPR/Cas9 as the first step for non-genetically modified (non-GM) P. ostreatus generation. Plasmids harboring expression cassettes of Cas9 and different single guide RNAs targeting fcy1 and pyrG were individually transferred into fungal protoplasts of the PC9 strain, which generated some strains exhibiting resistance to 5-fluorocytosine and 5-fluoroorotic acid, respectively. Genomic PCR followed by sequencing revealed small insertions/deletions or insertion of a fragment from the plasmid at the target site in some of the drug-resistant strains. The results demonstrated efficient CRISPR/Cas9-assisted genome editing in P. ostreatus, which could contribute to the molecular breeding of non-GM cultivated strains in the future. Furthermore, a mutation in fcy1 via homology-directed repair using this CRISPR/Cas9 system was also efficiently introduced, which could be applied not only for precise gene disruption, but also for insertions leading to heterologous gene expression in this fungus

Status of the RFQ linac installation and conditioning of the Linear IFMIF Prototype Accelerator

Abstract The Radio Frequency Quadrupole (RFQ) linac and 1.6 MW RF power system of the Linear IFMIF Prototype Accelerator (LIPAc) facility in the International Fusion Energy Research Center (IFERC) in Rokkasho (Japan) has been installed and conditioned. During the assembly and tuning process, the RFQ cavity was protected with a temporary tent from the potential deterioration of performance caused by dust. The vacuum in the cavity was improved through the 100 °C baking process of the cavity. The high power test of the 175 MHz RF systems up to 200 kW in CW for each of the eight RF chains was performed for checking its stable output reproducibility in Japan, before connecting 9–3/16 inch coaxial transmission lines from the RF chains to the RF input couplers of the cavity. It was confirmed that the eight RF chains provided the balanced RF power to the single RFQ cavity in-phase using a feedback loop and a synchronization system. The peak power in the cavity achieved 150 kW in the pulsed mode, which corresponds approximately to the required electric field to accelerate proton beam. Such RF conditioning process is ongoing to achieve 600 kW approximately required for deuteron beam commissioning planned in 2018