35 research outputs found
Adaptive Evolution of the Venom-Targeted vWF Protein in Opossums that Eat Pitvipers
The rapid evolution of venom toxin genes is often explained as the result of a biochemical arms race between venomous animals and their prey. However, it is not clear that an arms race analogy is appropriate in this context because there is no published evidence for rapid evolution in genes that might confer toxin resistance among routinely envenomed species. Here we report such evidence from an unusual predator-prey relationship between opossums (Marsupialia: Didelphidae) and pitvipers (Serpentes: Crotalinae). In particular, we found high ratios of replacement to silent substitutions in the gene encoding von Willebrand Factor (vWF), a venom-targeted hemostatic blood protein, in a clade of opossums known to eat pitvipers and to be resistant to their hemorrhagic venom. Observed amino-acid substitutions in venom-resistant opossums include changes in net charge and hydrophobicity that are hypothesized to weaken the bond between vWF and one of its toxic snake-venom ligands, the C-type lectin-like protein botrocetin. Our results provide the first example of rapid adaptive evolution in any venom-targeted molecule, and they support the notion that an evolutionary arms race might be driving the rapid evolution of snake venoms. However, in the arms race implied by our results, venomous snakes are prey, and their venom has a correspondingly defensive function in addition to its usual trophic role
Poisoning due to Philodryas olfersii (Lichtenstein, 1823) attended at Restauração Hospital in Recife, State of Pernambuco, Brazil: case report
Strategies in 'snake venomics' aiming at an integrative view of compositional, functional, and immunological characteristics of venoms
This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and
discusses how these may be combined through diverse experimental approaches with the goal of achieving a
more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms.
Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere
cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological
(antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary
strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails produced by
venomous snakes, responsible for thousands of deaths every year.Ministerio de EconomÃa y Competitividad/[BFU2013-42833-P]//EspañaUCR::VicerrectorÃa de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP