The type of adjuvant in whole inactivated influenza a virus vaccines impacts vaccine-associated enhanced respiratory disease

Influenza A virus (IAV) causes a disease burden in the swine industry in the US and is a challenge to prevent due to substantial genetic and antigenic diversity of IAV that circulate in pig populations. Whole inactivated virus (WIV) vaccines formulated with oil-in-water (OW) adjuvant are commonly used in swine. However, WIV-OW are associated with vaccine-associated enhanced respiratory disease (VAERD) when the hemagglutinin and neuraminidase of the vaccine strain are mismatched with the challenge virus. Here, we assessed if different types of adjuvant in WIV vaccine formulations impacted VAERD outcome. WIV vaccines with a swine δ1-H1N2 were formulated with different commercial adjuvants: OW1, OW2, nano-emulsion squalene-based (NE) and gel polymer (GP). Pigs were vaccinated twice by the intramuscular route, 3 weeks apart, then challenged with an H1N1pdm09 three weeks post-boost and necropsied at 5 days post infection. All WIV vaccines elicited antibodies detected using the hemagglutination inhibition (HI) assay against the homologous vaccine virus, but not against the heterologous challenge virus; in contrast, all vaccinated groups had cross-reactive IgG antibody and IFN-γ responses against H1N1pdm09, with a higher magnitude observed in OW groups. Both OW groups demonstrated robust homologous HI titers and cross-reactivity against heterologous H1 viruses in the same genetic lineage. However, both OW groups had severe immunopathology consistent with VAERD after challenge when compared to NE, GP, and non-vaccinated challenge controls. None of the WIV formulations protected pigs from heterologous virus replication in the lungs or nasal cavity. Thus, although the type of adjuvant in the WIV formulation played a significant role in the magnitude of immune response to homologous and antigenically similar H1, none tested here increased the breadth of protection against the antigenically-distinct challenge virus, and some impacted immunopathology after challenge

Interação nitrogênio, fósforo, potássio na cultura da mandioca no Norte de Mato Grosso.

Na Região Norte do Estado de Mato Grosso, foram testadas doses de adubação fosfatada (0, 40, 80 e 120 kg de P2O5/ha) em interação com nitrogênio (o e 40 kg de N/ha) e potássio (0 e 40 kg de K2O/ha) para a cultura da mandioca, em dois ciclos de cultivo, de novembro/81 a marco/83, com a cultivar "Cuiabana", e de outubro/82 a fevereiro/84, com a cultivar "Juriti". Determinou-se a produção de folhas, hastes, cepa, raiz, número de plantas e porcentagem de amido. Não foram observados diferenças significativas entre tratamento para nenhuma das variáveis nos dois ciclos.bitstream/item/81260/1/interacao-nitrogenio-Laercio-Duarte-Boletim-Pesquisa-9-1992.pdfMemória

Fitorreguladores de crescimento no alongamento in vitro de Pinus greggii.

Organizado por Patricia Póvoa de Mattos, Celso Garcia Auer, Rejane Stumpf Sberze, Katia Regina Pichelli e Paulo César Botosso

Métodos de extração de hidrocarbonetos policíclicos aromáticos em amostra de solo, sedimento e lodo.

bitstream/item/12265/1/documentos_79.pd

Components of multifractality in the Central England Temperature anomaly series

We study the multifractal nature of the Central England Temperature (CET) anomaly, a time series that spans more than 200 years. The series is analyzed as a complete data set and considering a sliding window of 11 years. In both cases, we quantify the broadness of the multifractal spectrum as well as its components defined by the deviations from the Gaussian distribution and the influence of the dependence between measurements. The results show that the chief contribution to the multifractal structure comes from the dynamical dependencies, mainly the weak ones, followed by a residual contribution of the deviations from Gaussianity. However, using the sliding window, we verify that the spikes in the non-Gaussian contribution occur at very close dates associated with climate changes determined in previous works by component analysis methods. Moreover, the strong non-Gaussian contribution found in the multifractal measures from the 1960s onwards is in agreement with global results very recently proposed in the literature.Comment: 21 pages, 10 figure