synthesis of copper ion incorporated uricase-based hybrid nanoflowers and their enhanced cataltic properties

Enzymehas been unique properties due to eco-friendly nature and superior catalyticactivity, high reaction specificity and low toxicity [1]. However, theirefficient use is limited due to some connatural disadvantages due to beingwithout stability, difficulty in recovery and reusability, loss of catalyticactivity after administration into the reaction and high cost. In order toovercome these disadvantages, several immobilization approaches have beendeveloped. The expectancy with enzyme immobilization is to improve bothcatalytic activity and stability [2]. Recently, hybrid materials, combiningwith the advantages of organic and inorganic components have been widelyapplied for enzyme immobilization because of their unique properties. Herein,we present a study on the formation, catalytic activity and stability of hybridnanoflowers (hNFs) containing uricase and copper ions.</p

preparation of maltase-inorganic hybrid nanoflowers and investigation of inflence on enzymatic activity of synthesis conditions

Enzymes have found widespread in variousscientific and technical fields, including chemistry, biochemistry, medicine,pharmaceutical science and industry [1]. Although enzymes have uniqueproperties, such as high catalytic activity, reaction specificity, stability,selectivity, low toxicity and water solubility, their efficient use isvery limited due to their innate disadvantages associated with instabilityduring storage, loss of catalytic activity after &nbsp;implementationinto the reaction, high production cost and lack of recovery from reactionmedium, substrate- and product-inhibitions [2]. &nbsp;In order to overcome these limitations,several immobilization approaches have been developed.Inthis study, &nbsp;maltase-copper phosphate hybrid nanoflowers(HNFs) synthesized and at the same time formation mecanism, catalytic activityand stability under different experimantal parameters (pHs, temperatures) wasinvestigated. ThehNFs were characterized using SEM, EDX analysis, Bradford assay, UV-Visspectrometry and XRD. The effect of reaction temperatures on the morphology ofthe hNFs was demonstrated with SEM images (Fig. 1).&nbsp;</p

Effect of a Synthesized Compound against Cancerous Cell Line and Synthesis of Copper Ion Incorporated 1-(3,4-Diaminophenyl) Ethanone-Based Hybrid Nanoflowers

Cancer is a very common disease between men and women in worldwide. Although manystudies have been conducted to find effective anticancer drugs by researchers, this disease is notcompletely exterminated. 2-Nitrilebenzyl substituted benzimidazolium salt was obtained inmoderate yield (Scheme 1). The cytotoxic effect of the synthesized ligand were tested against twohuman cancer cell lines and one non-cancerous cell line. According to obtained results; compound 2have anticancer effect. Furthermore; we conducted a study on the formation; catalytic activity andstability of hybrid nanoflowers (hNFs) containing benzimidazolium salt and copper ions. Thesynthesis of hNFs was accomplished using a modified method. In all activity measurementexperiments; the hNFs and an identical concentration of free benzimidazolium salt was used. Theactivities of hNFs were determined by colorimetric and spectroscopic methods using guaiacol as achromogenic substrate.The hNFs were characterized using SEM, EDX, FT-IR analysis, Bradfordassay, UV-Vis spectrometry and XRD. The effect of reaction temperatures on the morphology of thehNFs is demonstrated with SEM images (Figure 1).&nbsp;</p