Man, plant remains, diet: spread and ecology of Prunus L. in Sardinia

Archaeobotany offers the opportunity to explore the various practices related to the use of wild plants and understand how these have been selected for domestication. The identification of wild and cultivated plants could facilitate the understanding of the role they have in the subsistence economy and how this is strictly related to dietary habits. The main goals of this thesis are to interpret and understand the relation between human communities and the exploitation of plant resources in the past, thus understanding agricultural and alimentary behaviors in the present time. The genus Prunus has been selected because understudied in Sardinia from the archaeobotanical point of view but fairly widespread in the Mediterranean archaeological contexts. The application of image analysis technique for an adequate definition of the endocarp morpho-colorimetric and morphometric parameters represents an important diagnostic factor in the Prunus taxonomy studies and consequently may be helpful for the improvement of the management and in the enhancement of ancient fruit. The study of traditional varieties aims to create models of development, recovery and enhancement of ancient fruit. Also sets the basis for proposals for the recovery of cultural traditions and local economies. For the first time, it was possible to investigate about the morphology and morphometry of P. domestica endocarps of traditional local varieties from Sardinia. Endocarp morpho-colorimetric features, EFDs and Haralick’s descriptors obtained by image analysis allowed to implement a statistical classifier able to identify and classify the studied varieties of P. domestica, identifying plausible synonymy groups and confirming that the endocarp retain some characters directly related to the fruit skin color. The discovery of well-preserved waterlogged endocarps of P. domestica from the Phoenician-Punic settlement of Santa Giusta could be evidence that the introduction of primitive cultivated forms of plums in Sardinia have been introduced by the Phoenicians people. Moreover, these endocarps represent the oldest findings and they are the oldest evidence of cultivated plums in the western Mediterranean Basin. Finally, for the first time, it was possible to investigate through image analysis system about the morphology and morphometry of archaeological P. spinosa endocarps from Sardinia. These sites are currently the only finds of P. spinosa remains in waterlogged conditions documented in Sardinia by archaeological sources. Thanks to image analysis system it was possible to understand the hypothetical origin

Morphological Characterization of Fossil Vitis L. Seeds from the Gelasian of Italy by Seed Image Analysis

The discovery of well-preserved fossil Vitis L. seeds from the Gelasian stage in Italy has provided a unique opportunity to investigate the systematics of fossilized Vitis species. Through seed image analyses and elliptical Fourier transforms of fossil Vitis seeds from the sites Buronzo-Gifflenga and Castelletto Cervo II, we pointed out a strong relationship to the group of extant Eurasian Vitis species. However, classification analyses highlighted challenges in accurately assigning the fossil grape seeds to specific modern species. Morphological comparisons with modern Vitis species revealed striking similarities between the fossil seeds and V. vinifera subsp. sylvestris, as well as several other wild species from Asia. This close morphological resemblance suggests the existence of a population of V. vinifera sensu lato in Northen Italy during the Gelasian. These findings contributed to our understanding of the evolution and the complex interplay between ancient and modern Vitis species

Germination of Sardinian black and white Vitis vinifera seeds according to treatments and dormancy factors

Physiological dormancy of Vitis vinifera seeds jeopardises breeding programs and biodiversity evolution. To increase the knowledge on dormancy breaking, seeds of white and black Sardinian grape cultivars (cvs) were exposed to different pre-germination treatments. To shed light on the physiological and structural factors involved in seed dormancy, the contents of oil, abscisic acid, gibberellic acid, 3-indolacetic acid, condensed tannins, and total polyphenols were determined. In addition, sectioned seeds were observed by SEM to determine the morphological and anatomical characteristics. Dormancy break in white, but not in black grape seeds, occurred under almost all imposed pre-germination treatments. Among red cvs, only seeds from ‘Cagnulari’ germinated when kept at 25 °C. Chilling seeds of the white cvs ‘Malvasia sarda’ and ‘Vernaccia di Oristano’ for 30 d resulted in the most effective treatment. Compared to white cvs, seeds of red ones owned 7 times higher levels of abscisic acid however, gibberellic acid content resulted 4 times less. Concerning the coat characteristics, red cv seeds had a thicker cuticle (6-10 μm) than white (4-6 μm) ones, however the most significant diversities were found for the inner integument, where in addition to size variances, palisade cell wall were structurally different

Unearthing Grape Heritage: Morphological Relationships between Late Bronze–Iron Age Grape Pips and Modern Cultivars

The grapevine was one of the earliest domesticated fruit crops and has been cultivated since ancient times. It is considered one of the most important fruit crops worldwide for wine and table grape production. The current grape varieties are the outcome of prolonged selection initiated during the domestication process of their wild relative. Recent genetic studies have shed light on the origins of the modern domestic grapevine in western Europe, suggesting that its origin stems from the introgression between eastern domestic grapes and western wild grapes. However, the origin of ancient grapevines remains largely unexplored. In this study, we conducted an extensive analysis of 2228 well-preserved waterlogged archaeological grape pips from two sites in Sardinia (Italy), dated to the Late Bronze Age (ca. 1300-1100 BC) and the Iron Age (4th and 3rd centuries BC). Using morphometrics and linear discriminant analyses, we compared the archaeological grape pips with modern reference collections to differentiate between wild and domestic grape types and to investigate similarities with 330 modern cultivars. Grape pips from the Late Bronze Age displayed a high percentage of similarity with domesticated grapevines, with a small percentage assigned to wild ones, while the majority of grape pips from the Iron Age were classified as domestic. Discriminant analyses revealed that both white and red grape varieties were cultivated during the Late Bronze and Iron Ages, suggesting a high level of diversification in grape cultivation. Furthermore, a high percentage of archaeological grape pips from both periods showed strong similarities with modern cultivars from the Caucasus and Balkans. This suggests that the great diversity of grapevines present in Sardinia could result from interbreeding between western Asian cultivars and local grapevines that began in the Late Bronze Age. Additionally, a substantial proportion of archaeological grape pips exhibited similar morphometric characteristics to two important Mediterranean grape cultivars: "Muscat à petits grains blancs" and "Garnacha"

Domestic grape germination behaviour: the ‘Chardonnay’ and ‘Syrah’international cultivars’s study case

The domestic grape germination eco-physiology is a little studied aspect since reproduction occurs predominantly agamically, despite the sexual reproduction remains the main form for the selection of new cultivars. In this study, two international cultivars grown all over the world-Chardonnay and Syrah-were chosen as models of the domestic grape for the experiments in the eco-physiology of germination. The experimental design consisted of chemical, mechanical pre-treatments and combination of them as the bird ingestion pre-treatment that simulates the transit through the digestive tract of birds. Furthermore, seeds were submitted to different periods of cold stratification -0, 15, 30, 60 and 90 days -to simulate the winter effect. Seeds were placed to germinate at different incubation temperatures, to find the optimal germination protocol. The results showed that domestic grapes retain the need for cold stratification, and the best germination temperature is represented by the fluctuating temperature that simulates spring conditions. Our results help to understand what the best germination conditions of domestic grapes are and offer a contribution to extend the knowledge on how the process of domestication may have affected the biology of Vitis vinifera L. subsp. vinifera

First finds of Prunus domestica L. in Italy from the Phoenician and Punic periods (6th-2nd centuries BC)

Abstract During the archaeological excavations in the Phoenician and Punic settlement of Santa Giusta (Oristano, Sardinia, Italy), dating back to the 6th–2nd centuries bc, several Prunus fruitstones (endocarps) inside amphorae were recovered. The exceptional state of preservation of the waterlogged remains allowed morphometric measurements to be done by image analysis and statistical comparisons made with modern cultivated and wild Prunus samples collected in Sardinia. Digital images of modern and archaeological Prunus fruitstones were acquired with a flatbed scanner and analysed by applying image analysis techniques to measure 26 morphometric features. By applying stepwise linear discriminant analysis, a morphometric comparison was made between the archaeological fruitstones of Prunus and the modern ones collected in Sardinia. These analyses allowed identification of 53 archaeological fruitstones as P. spinosa and 11 as P. domestica. Moreover, the archaeological samples of P. spinosa showed morphometric similarities in 92.5% of the cases with the modern P. spinosa samples currently growing near the Phoenician and Punic site. Likewise, the archaeological fruitstones identified as P. domestica showed similarities with the modern variety of P. domestica called Sanguigna di Bosa which is currently cultivated near the village of Bosa. Currently, these findings represent the first evidence of P. domestica in Italy during the Phoenician and Punic periods. Keywords Archaeobotany · Image analysis · Morphometric features · Prunus · Sardini

The Botanical Record of Archaeobotany Italian Network - BRAIN: a cooperative network, database and website

Con autorización de la revista para autores CSIC[EN] The BRAIN (Botanical Records of Archaeobotany Italian Network) database and network was developed by the cooperation of archaeobotanists working on Italian archaeological sites. Examples of recent research including pollen or other plant remains in analytical and synthetic papers are reported as an exemplar reference list. This paper retraces the main steps of the creation of BRAIN, from the scientific need for the first research cooperation to the website which has a free online access since 2015.Peer reviewe

Discovering Plum, Watermelon and Grape Cultivars Founded in a Middle Age Site of Sassari (Sardinia, Italy) through a Computer Image Analysis Approach

The discovery of several waterlogged plant remains in a Middle Ages context (1330–1360 AD) in Sassari (NS, Sardinia, Italy) enabled the characterisation of archaeological plum fruit stones and watermelon and grape seeds through computer image analysis. Digital seed/endocarp images were acquired by a flatbed scanner and processed and analysed by applying computerised image analysis techniques. The morphometric data were statistically elaborated using stepwise linear discriminant analysis (LDA), allowing comparisons among archaeological remains, wild populations and autochthonous cultivars. Archaeological samples of plum were compared with 21 autochthonous cultivars of Prunus domestica from Sardinia, while archaeological watermelon seeds were compared with 36 seed lots of Citrullus from Europe, Africa and Asia. Moreover, archaeological grape seeds were compared with 51 autochthonous traditional cultivars of Vitis vinifera subsp. vinifera from Sardinia, 16 cultivars from Tuscany, six cultivars from Liguria, and eight cultivars from Catalonia (Spain). Archaeological plum remains showed morphological affinity with five cultivars of Sardinia. Seed features of the archaeological watermelon remains demonstrated affiliation with a proper sweet dessert watermelon, Citrullus lanatus, and similarity with some Sardinian cultivars. Regarding the archaeological remains of grape, morphometric comparisons showed a high similarity with autochthonous cultivars from Catalonia and Liguria. This study provides new information about ancient fruit cultivated and consumed during the Middle Ages in Sardinia