State of the body in disorders of diurnal physiological rhythms and long-term hypokinesia

In order to study the effects of hypokinesia and circadian rhythm restructuring on the morphological and functional status of the hypothalamo-hypophysic-adrenal system, young male Wistar rats were placed in small cages for varying periods. The animals were decapitated and preparations were made from sections of the brain and adrenals and numerous destructive changes were noted in the investigated regions of the brain, indicating that the condition of these areas is directly affected by disruption of established rhythms in physiological processes

Functional-morphological parallels of the hypothalamo-pituitary-adrenal system response reaction to long-term hypokinesia

The effect of 2 and 4 week hypokinesia regimens on the hypothalamo-pituitary-adrenal system (HPAS) was investigated in 110 inbred mice. Progressive exhaustion and pathological reorganization of the HPAS morphofunctional structures was revealed. On the basis of established facts of interlineary and interspecies differences in the HPAS response, it is suggested that the animal body response reaction to the long term effects of hypokinesia depends largely on its HPAS resistance and the values of this system's defensive adaptation potential

Wire GEM detector

A wire GEM (WGEM) detector with a gas gap between meshes was constructed. The detector provides the amplification 5x10E5 for the gas mixture of Ar +20% CO2 at atmospheric pressure. As compared with well-known GEM detectors produced by perforation the plastic plate metalized on both sides the WGEM does not suffer from breakdowns between its electrodes and the effect of accumulation of charges on holes walls is absent. As a result the WGEM has high reliability and stability.Comment: Presented at the RD51 Collaboration Meeting, CERN, November 2009, submitted to the Prib. Tech. Expe

RETGEM with polyvinylchloride (PVC) electrodes

This paper presents a new design of the RETGEM (Resistive Electrode Thick GEM) based on electrodes made of a polyvinylchloride material (PVC). Our device can operate with gains of 10E5 as a conventional TGEM at low counting rates and as RPC in the case of high counting rates without of the transit to the violent sparks. The distinct feature of present RETGEM is the absent of the metal coating and lithographic technology for manufacturing of the protective dielectric rms. The electrodes from PVC permit to do the holes by a simple drilling machine. Detectors on a RETGEM basis could be useful in many fields of an application requiring a more cheap manufacturing and safe operation, for example, in a large neutrino experiments, in TPC, RICH systems.Comment: Presented at the RD51 Collaboration Meeting, CERN, November 200

MARs Wars: heterogeneity and clustering of DNA-binding domains in the nuclear matrix

Aim. CO326 is a chicken nuclear scaffold/matrix attachment region (MAR) associated with the nuclear matrix in several types of chicken cells. It contains a binding site for a sequence-specific DNA-binding protein, F326. We have studied its interaction with the nuclear matrix. Methods. We have used an in vitro MAR assay with isolated matrices from chicken HD3 cells. Results. We have found that an oligonucleotide binding site for the F326 inhibits binding of the CO326 to the nuclear matrix. At the same time, the binding of heterologous MARs is enhanced. Conclusions. Taken together, these data suggest that there exist several classes of MARs and MAR-binding domains and that the MAR-binding proteins may be clustered in the nuclear matrix

Non-backtracking walks reveal compartments in sparse chromatin interaction networks

Chromatin communities stabilized by protein machinery play essential role in gene regulation and refine global polymeric folding of the chromatin fiber. However, treatment of these communities in the framework of the classical network theory (stochastic block model, SBM) does not take into account intrinsic linear connectivity of the chromatin loci. Here we propose the "polymer" block model, paving the way for community detection in polymer networks. On the basis of this new model we modify the non-backtracking flow operator and suggest the first protocol for annotation of compartmental domains in sparse single cell Hi-C matrices. In particular, we prove that our approach corresponds to the maximum entropy principle. The benchmark analyses demonstrates that the spectrum of the polymer non-backtracking operator resolves the true compartmental structure up to the theoretical detectability threshold, while all commonly used operators fail above it. We test various operators on real data and conclude that the sizes of the non-backtracking single cell domains are most close to the sizes of compartments from the population data. Moreover, the found domains clearly segregate in the gene density and correlate with the population compartmental mask, corroborating biological significance of our annotation of the chromatin compartmental domains in single cells Hi-C matrices

The upstream area of the chicken α-globin gene domain is transcribed in both directions in the same cells

AbstractIt was demonstrated previously that in erythroid chicken cells an extended upstream area of the α-globin gene domain is transcribed in both directions as a part of ggPRX gene and a part of a full domain transcript of the α-globin gene domain. Here, we show that both DNA chains of the above-mentioned region are transcribed in the same cells and that the corresponding transcripts coexist in nuclei. The data obtained suggest that cells possess a molecular mechanism which in some cases prevents the formation of dsRNA and subsequent destruction of both transcripts in spite of the presence of complementary RNA chains in the cell nucleus

Formation of Multicharged Ions at Quasi-Gasdynamic Plasma Confinement in a Mirror trap

It was shown in [1] that an increase in plasma density Ne in sources of multicharged ions leads to a substantial increase of ion current and improves slightly the ion distribution over charge states. Validity of this statement was verified in experiments with plasma densities not exceeding several units of 1012 cm-3. It was revealed [2] that, for the electron densities exceeding 1013 cm-3, the regime of plasma confinement in a trap changes significantly, the scaling described in [1] is no longer valid, and the quasi-gasdynamic regime of plasma confinement is realized. The plasma confinement time ti in this regime weakly depends on electron density. Consequently, the parameter governing formation of multicharged ions, Neti , grows as the electron density is increased. This means that an increase in plasma density results not only in an increase in the total ion current but also in the shift of the ion charge state distribution towards higher charge states. The present work concerns experimental investigation of the quasi-gasdynamic regime of confinement of a hot plasma in a direct magnetic trap and formation of multicharged ions in this regime. Experiments were conducted on the setup described in detail in [3]. Millimeter wave radiation with maximum power W=130 kW, frequency f=37.5 GHz, and pulse duration up to 1.5 ms was focused along magnetic field lines into a simple mirror trap with mirror ratio 3.4, length 25 cm, and maximum magnetic field 2.5 T. The temperature and density of the electrons were determined from spectral analysis of X-ray bremsstrahlung of plasma in the 2-20 keV range and from the transmission factor of diag-nostic microwave radiation through the plasma. It is concluded that a quasi-gasdynamic regime of plasma confinement is realized. Ion distribution over charge states in the quasi-gasdynamic regime is calculated and the re-sults obtained are compared with experimental data. A strong effect of anisotropy of the electron distribution function over energies on the efficiency of plasma confinement and formation of multicharged ions is considered. Problems of plasma stability in an axisymmetric mirror trap un-der powerful microwave pumping are addressed

RPC with low-resistive phosphate glass electrodes as a candidate for the CBM TOF

Usage of electrodes made of glass with low bulk resistivity seems to be a promising way to adapt the Resistive Plate Chambers (RPC) to the high-rate environment of the upcoming CBM experiment. A pilot four-gap RPC sample with electrodes made of phosphate glass, which has bulk resistivity in the order of 10^10 Ohm cm, has been studied with MIP beam for TOF applications. The tests have yielded satisfactory results: the efficiency remains above 95% and the time resolution stays within 120 ps up to the particle rate of 18 kHz/cm2. The increase in rate from 2.25 to 18 kHz/cm2 leads to an increase of estimated "tails" fraction in the time spectrum from 1.5% to 4%.Comment: 8 pages, 6 figures, submitted to Elsevier Scienc

Radio Emission and Particle Acceleration in SN 1993J

The radio light curves of SN 1993J are found to be well fit by a synchrotron spectrum, suppressed by external free-free absorption and synchrotron self-absorption. A standard r^-2 circumstellar medium is assumed, and found to be adequate. The magnetic field and number density of relativistic electrons behind the shock are determined. The strength of the magnetic field argues strongly for turbulent amplification behind the shock. The ratio of the magnetic and thermal energy density behind the shock is ~0.14. Synchrotron and Coulomb cooling dominate the losses of the electrons. The injected electron spectrum has a power law index -2.1, consistent with diffusive shock acceleration, and the number density scales with the thermal electron energy density. The total energy density of the relativistic electrons is, if extrapolated to gamma ~ 1, ~ 5x10^-4 of the thermal energy density. The free-free absorption required is consistent with previous calculations of the circumstellar temperature of SN 1993J, T_e ~ (2-10)x10^5 K. The relative importance of free-free absorption, Razin suppression, and the synchrotron self-absorption effect for other supernovae are briefly discussed. Guidelines for the modeling and interpretation of VLBI observations are given.Comment: accepted for Ap.