Protease production and enzymatic soaking of salt-preserved buffalo hides for leather processing

Response surface methodological (RSM) optimization of protease by Pseudomonas aeruginosa MCM B327, increased 1.3-fold activity with 1% inoculum having cell density of 27.57 x 109 cells mL-1 at pH 7, 300C and 72 h of incubation. Protease enzyme recovered from P. aeruginosa showed characteristic activities against diverse proteins of hide. Enzyme was found to be active with substrates e.g. casein, Bovine serum albumin, gelatin, elastin, haemoglobin but inactive against keratin and collagen. During leather manufacturing, non-collagenase and non-keratinase activities have advantageous in a quality leather and hair saving process, respectively. Increased proteolytic enzyme concentration (0.1-0.5%) in soaking process showed increased water penetration because of hydrolysis of albumin and elastin proteins as indicated by opened fibers in histopathological sections. These findings suggest, protease secreted by P. aeruginosa may have application in soaking operation of leather processing for minimizing harmful deharing chemicals and processing time

Enzymatic Depilation of Animal Hide: Identification of Elastase (LasB) from Pseudomonas aeruginosa MCM B-327 as a Depilating Protease

Conventional leather processing involving depilation of animal hide by lime and sulphide treatment generates considerable amounts of chemical waste causing severe environmental pollution. Enzymatic depilation is an environmentally friendly process and has been considered to be a viable alternative to the chemical depilation process. We isolated an extracellular protease from Pseudomonas aeruginosa strain MCM B-327 with high depilation activity using buffalo hide as a substrate. This 33 kDa protease generated a peptide mass fingerprint and de novo sequence that matched perfectly with LasB (elastase), of Pseudomonas aeruginosa. In support of this data a lasB mutant of MCM B-327 strain lacked depilatory activity and failed to produce LasB. LasB heterologously over-produced and purified from Escherichia coli also exhibited high depilating activity. Moreover, reintroduction of the lasB gene to the P. aeruginosa lasB mutant via a knock-in strategy also successfully restored depilation activity thus confirming the role of LasB as the depilating enzyme

Effect of different processing methods on resistant starch content and in vitro starch digestibility of some common Indian pulses

In the present study, the effect of various processing methods like soaking, autoclaving, storage and pullulanase treatment on resistant starch content and in vitro starch digestibility of dhals (split pulses) of pulses pigeon pea, green gram and black gram was assessed. Results showed that these processing methods including pullulanase treatment significantly decreased the resistant starch (RS) content in all the samples. Further, results of in vitro starch digestibility demonstrated that autoclaving significantly increased the predicted glycemic index (pGI) and slowly digested starch (SDS). However, decrease in rapidly digested starch (RDS) and starch digestive index (SDI) was observed after autoclaving. Results suggested that RS and RDS are getting converted into SDS after processing treatments. Moreover, present findings suggest that, the processed dhals of pigeon pea, green gram and black gram could have added health promoting potential due to their high content of SDS

Effect of different processing methods on resistant starch content and <i style="mso-bidi-font-style:normal">in vitro</i> starch digestibility of some common Indian pulses

541-546In the present study, the effect of various processing methods like soaking, autoclaving, storage and pullulanase treatment on resistant starch content and in vitro starch digestibility of dhals (split pulses) of pulses pigeon pea, green gram and black gram was assessed. Results showed that these processing methods including pullulanase treatment significantly decreased the resistant starch (RS) content in all the samples. Further, results of in vitro starch digestibility demonstrated that autoclaving significantly increased the predicted glycemic index (pGI) and slowly digested starch (SDS). However, decrease in rapidly digested starch (RDS) and starch digestive index (SDI) was observed after autoclaving. Results suggested that RS and RDS are getting converted into SDS after processing treatments. Moreover, present findings suggest that, the processed dhals of pigeon pea, green gram and black gram could have added health promoting potential due to their high content of SDS

Comparative functional potential of some plant materials in India

159-166Soluble dietary fibers are known to play an important role in maintaining health. These may have prebiotic activity and additionally their basic physico-chemical properties may contribute to their multifunctional health potential. Present study was planned with 30 plant materials grouped as fruits, vegetables, and other tuber crops using gum acacia as standard, being a known dietary fiber having prebiotic potential. The materials were studied for water holding capacity, cholesterol and bile salt binding activity, α-amylase inhibition and trypsin inhibition activity. Fifteen plant materials showed cholesterol binding capacity significantly higher than gum acacia by 0.7 to 86 %.Nineteen plant materials were found to have bile salt binding capacity (27-99 %) higher than gum acacia (24 %). Water holding capacity was observed in the range of 2.71 to 35.4 g/ g dry material for all the materials, while gum acacia had the least value of 0.82 g/ g dry weight. Sixteen materials showed α-amylase inhibition in the range of 9.99-95.0 % as compared to 9.65 % of gum acacia. Trypsin inhibition activity showed in the range of 49-98 %. This is the first report of multifunctional activities of plant materials with prebiotic potential. The results may help to develop prebiotic formulations having multifunctional potential for treatment of gastrointestinal disorders

Comparative functional potential of some plant materials in India

Soluble dietary fibers are known to play an important role in maintaining health. These may have prebiotic activity and additionally their basic physico-chemical properties may contribute to their multifunctional health potential. Present study was planned with 30 plant materials grouped as fruits, vegetables, and other tuber crops using gum acacia as standard, being a known dietary fiber having prebiotic potential. The materials were studied for water holding capacity, cholesterol and bile salt binding activity, α-amylase inhibition and trypsin inhibition activity. Fifteen plant materials showed cholesterol binding capacity significantly higher than gum acacia by 0.7 to 86 %.Nineteen plant materials were found to have bile salt binding capacity (27-99 %) higher than gum acacia (24 %). Water holding capacity was observed in the range of 2.71 to 35.4 g/ g dry material for all the materials, while gum acacia had the least value of 0.82 g/ g dry weight. Sixteen materials showed α-amylase inhibition in the range of 9.99-95.0 % as compared to 9.65 % of gum acacia. Trypsin inhibition activity showed in the range of 49-98 %. This is the first report of multifunctional activities of plant materials with prebiotic potential. The results may help to develop prebiotic formulations having multifunctional potential for treatment of gastrointestinal disorders

Scholars Journal of Medical Case Reports ISSN 2347-9507 (Print) Unusual Presentation of Arthritis in an Immunodefecient Child

Abstract: Arthritis may be the first clinical manifestation of primary hypogammaglobulinaemia. We report a case of primary hypogammaglobulinemia who presented with migratory polyarthritis and sore throat. The diagnosis of primary hypogammaglobulinemia (Brutons disease) was delayed as it was previously thought it was migratory polyarthritis, due to Rhematic fever, Which is very common in Asian countries. These patients are likely to develop recurrent infections complicated by arthritis. This delay resulted in considerable joint destruction. The measurement of serum immunoglobulin concentrations readily differentiates immunodeficiency from conditions such as Stili&apos;s disease and dictates subsequent management. The importance of presenting this case was that one should be in look out for something more in cases where child is not responding well to antibiotics and getting various bacterial infections on and off