Study of drug utilization in cardiovascular emergency at a rural teaching hospital in Tamilnadu, India

Background: Cardiovascular disease is a common term which encompasses a range of disorders that affect the heart. Cardiovascular disease is the leading cause of death among men and women in India and across the globe. There is evidence of irrational drug use in cardiovascular disorders. Drug utilization study is used to evaluate the rationality of medication use. The objective of this study was to observe the most frequently treated emergency cardiovascular diseases and to study the prescribing pattern among inpatients admitted in CICU Unit of tertiary care teaching hospital.Methods: A retrospective, observational drug utilization study was designed to evaluate the drug prescribing pattern in cardiovascular emergency patients admitted in CICU unit of tertiary care teaching hospital. Study was carried out for a duration of 4 months. Data were collected from patient case reports after getting permission from Medical superintendent of hospital. Prescribing pattern of drugs were studied based on WHO indicators.Results: Total 150 patients were included in the study. Most common cardiovascular emergency reported was Myocardial infarction (60.66%) followed by Angina pectoris (24.66%). The most common comorbidities found along with cardiovascular emergency were hypertension (62.66%) followed by Diabetes mellitus (15.33%). Most prescribed cardiovascular drugs were from the category of hypolipidemics (94.66%) and anti-platelets (92.66%). Average number of drugs per prescription was found to be 9.42. Average hospital stay per patient was found to be 4.25 days. Percentage of drugs prescribed by generic name was 70.66%. Percentage of drugs prescribed from essential drug list and National list of essential medicines were 87.4% and 97.23% respectively.Conclusions: All the drugs prescribed were according to the current guidelines. Majority of the drugs were prescribed in generic form

Time resolved area normalized emission spectroscopy (TRANES) of DMABN confirms emission from two states

4-N, N-Dimethylaminobenzonitrile (DMABN) is a simple molecule which is extensively studied to understand the excited state kinetics and the origin of time dependent fluorescence in several organic solvents. We use a recently described method, time resolved area normalized emission spectroscopy (TRANES), for the analysis of wavelength dependent fluorescence of DMABN in acetonitrile and 1,4-dioxane. An isoemissive point was observed in the TRANES spectra, which confirms that there are only two emissive species A∗ and B∗:A→A∗⇔B∗

TRANES analysis of the fluorescence of nile red in organized molecular assemblies confirms emission from two species

Time-resolved area normalized emission spectroscopy (TRANES) is a new method for the analysis of fluorescence of dyes in complex chemical and biological systems (A S R Koti, M M G Krishna and N Periasamy, 2001,J. Phys. Chem. 105, 1767). The model-free method extends the power of time-resolved emission spectroscopy (TRES) analysis and removes the ambiguity in the interpretation when the emission spectrum is time-dependent. Observation of an isoemissive point in TRANES analysis of fluorescence is an unambiguous indication for the presence of two emissive species in the sample. The isoemissive point occurs at a wavelength where the ratio of the radiative rates of the two species is equal to the ratio of their total radiative rates. The polarity-sensitive nile red dye shows time-dependent emission spectra in the organized bilayer assemblies of TX micelle and bilayer egg-phosphotidylcholine (egg-PC) membrane. Time-dependent spectra in complex systems support many important models (solvation model and heterogeneity in the ground and/or excited state). TRANES analysis shows that the fluorescence emission of nile red in TX micelle and egg-PC membrane is due to two emissive species solubilized in different sites

Solvent exchange in excited-state relaxation in mixed solvents

The fluorescence of styrylthiazoloquinoxaline (STQ) in the solvent mixture methanol and dichloromethane (DCM) and 2-octanol have many common characteristics: biexponential fluorescence decay, wavelength-dependent amplitudes, a negative amplitude for the short-lifetime component at long emission wavelengths, and a time-dependent red shift of the emission spectrum. In octanol, the fluorescence lifetime decreases with increasing temperature, whereas the lifetime increases with temperature in the methanol/DCM mixture. The fluorescence characteristics in 2-octanol (η=7.29 cP) are readily explained by the conventional model of excited-state relaxation kinetics by solvent reorientation. This model is not applicable for low-viscosity (η=0.455 cP) solvent mixtures. A model of excited-state relaxation kinetics involving solvent exchange (versus solvent reorientation in pure solvents) in the excited state is proposed for the solvent mixture. The model assumes that the solvent compositions around the solute are different in the ground and excited states and the solvent composition is temperature dependent

Desuperheater Temperature Control System Using Distributed Control System (DCS)

The Control System for Desuperheater Temperature of Boiled water is a critical task in industry. In this Paper reviews the study and collection of measurement datas from in TNPL ( Tamil Nadu Newsprint and Papers Limited) Power Plant , there are three sections namely Deaerator tank, Boiler and Turbine. I have done this work in the turbine section. The steam f rom the Boiler is at very High Temperature. The temperature of this high temperature steam is lowered by spraying the oxygen dissolved water using Desuperheater. The spraying water is controlled by Distributed Control System ( DCS ). The Input to the turbine is at High Temperature and High Pressure steam. The output of the turbine is mentioned in Mega Watts. When the superheated steam is given to the turbine then the Temperature and Pressure output steam condition is Low. The Low level steam is fed to the Desuperheater depends upon requirements in the various units. It is supplied to each section in TNPL

Study on Prevalence and Surgical Management of Haemorrhoid’s in a Tertiary Care Teaching Hospital in a Rural Area

Background: Haemorrhoids, which is also termed as piles characterized by the enlarged and bulged blood vessels surrounding the supporting tissues present in the anal canal of an individual. In the present study, an attempt has been made to find the prevalence of haemorrhoids and surgical management done in our geographical area. Methods: The present study was retrospective, cross-sectional study from the period of 1st January 2013 to 31st December 2017. A total of 351 haemorrhoid’s patients were admitted in inpatient surgical ward during that period. The demographic details of all the patients were noted from the medical records. Results: A total 219 (62%) patients were male and 132 (38%) were females. Most of the patients are predominantly adults above the age of 21 years. The post-operative stay in hospital is almost less than 10 days as it indicates that most of the patients were discharged without any complication. Conclusions: The male patients are predominant compared to female patients. The surgical complications are less. The exact prevalence rate may be high as some patients may either undergo a self-treatment or native medicine treatmen

Real Time, Spatial, and Temporal Mapping of the Distribution of c-di-GMP during Biofilm Development

© 2017 by The American Society for Biochemistry and Molecular Biology, Inc. Bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di- GMP) is a dynamic intracellular signaling molecule that plays a central role in the biofilm life cycle. Current methodologies for the quantification of c-di-GMP are typically based on chemical extraction, representing end point measurements. Chemical methodologies also fail to take into consideration the physiological heterogeneity of the biofilm and thus represent an average c-di-GMP concentration across the entire biofilm. To address these problems, a ratiometric, image-based quantification method has been developed based on expression of the green fluorescence protein (GFP) under the control of the c-di-GMPresponsive cdrA promoter (Rybtke, M. T., Borlee, B. R., Murakami, K., Irie, Y., Hentzer, M., Nielsen, T. E., Givskov, M., Parsek, M. R., and Tolker-Nielsen, T. (2012) Appl. Environ. Microbiol. 78, 5060-5069). The methodology uses the cyan fluorescent protein (CFP) as a biomass indicator and the GFP as a c-di-GMP reporter. Thus, the CFP/GFP ratio gives the effective c-di-GMP per biomass. A binary mask was applied to alleviate background fluorescence, and fluorescence was calibrated against known c-di-GMP concentrations. Using flow cells for biofilm formation, c-di-GMP showed a non-uniform distribution across the biofilm, with concentrated hot spots of c-di- GMP. Additionally, c-di-GMP was found to be localized at the outer boundary of mature colonies in contrast to a uniform distribution in early stage, small colonies. These data demonstrate the application of a method for the in situ, real time quantification of c-di-GMP and show that the amount of this biofilmregulating second messenger was dynamic with time and colony size, reflecting the extent of biofilm heterogeneity in real time

Assessment of drug utilization among geriatric patients based on defined daily dose concept in a rural tertiary care teaching hospital

Background: The objectives of the study were to use the anatomical therapeutic chemical classification (ATC) /defined daily dose (DDD) concept to study the drug utilization pattern in geriatric patients in a rural tertiary care teaching hospital.Methods: An observational study was conducted at Rajah Muthiah Medical College Hospital (OPD and IPD) over a period of six months from November 2018 to April 2019. The data was collected from 204 patients using specially designed data collection form. The patients were selected based on inclusion and exclusion criteria. Results: A total of 204 patients were included in this study. The study population consisting of males 130 (63.7%) and females 74 (36.2%). Diseases related to the cardiovascular system 67 (32.84%) were the most common cause for the geriatric patients to attend the hospital, followed by surgical diseases 47 (23.03%), Oncological diseases 2 (0.98%) were the least frequency encountered. The average number of drugs prescribed per prescription was 8.79. Out of 1795 drugs prescribed, 60.86% of drugs were prescribed in generic form, and 39.14% were prescribed in brand name. The study analysed that 71.25% of drugs prescribed were from essential drug list (EDL) 2016-2018. Number of prescriptions with an injection was 74.04%. Paracetamol (N02B01) was the most frequently prescribed drug, followed by Amlodipine (C08CA01), Dexamethasone (H02AB02), Clopidogrel (B01AC04), Ferrous sulfate (B03AA07), Acetylsalicylic acid (B01AC06), Hydrocortisone (H02AB09), Tamsulosin (G04CA02), Atorvastatin (C10AA05), Furosemide (C03CA01).Conclusions: Drug utilization study can help in evaluating the quality of care given to the geriatric patients and promote rational use of medicines

Metastases to the craniovertebral junction: illustrative case report and review of literature

Metastases at the craniovertebral junction represent 0.5-1% of spinal metastatic lesions. Common primary sites include breast, lung and prostate carcinoma. Initial presenting features include neck pain and occipital neuralgia. High index of suspicion is required to recognize this entity in patients presenting with neck pain. If left unrecognized, these metastatic lesions have the propensity to cause catastrophic collapse leading to significant morbidity and mortality due to fracture subluxation and spinal cord compression. Here we discuss such a patient who presented with acute onset quadriparesis and lower cranial nerve palsies due to metastatic lesion involving the C1 and C2 vertebra causing medullary and cervical cord compression. Early detection and timely intervention are key to improving outcomes in such patients

Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000RAD and 15000RAD

The availability of genomic resources including linkage information for camelids has been very limited. Here, we describe the construction of a set of two radiation hybrid (RH) panels (5000RAD and 15000RAD) for the dromedary (Camelus dromedarius) as a permanent genetic resource for camel genome researchers worldwide. For the 5000RAD panel, a total of 245 female camel-hamster radiation hybrid clones were collected, of which 186 were screened with 44 custom designed marker loci distributed throughout camel genome. The overall mean retention frequency (RF) of the final set of 93 hybrids was 47.7%. For the 15000RAD panel, 238 male dromedary-hamster radiation hybrid clones were collected, of which 93 were tested using 44 PCR markers. The final set of 90 clones had a mean RF of 39.9%. This 15000RAD panel is an important high-resolution complement to the main 5000RAD panel and an indispensable tool for resolving complex genomic regions. This valuable genetic resource of dromedary RH panels is expected to be instrumental for constructing a high resolution camel genome map. Construction of the set of RH panels is essential step toward chromosome level reference quality genome assembly that is critical for advancing camelid genomics and the development of custom genomic tools