Professional cultures: New Zealand occupational therapists experience of an international internship

Mary Butler, Fenja Jones, and Susan E. Rya

Natural resources of the Barron River catchment 1: stream habitat, fisheries resources and biological indicators

The Barron River drains into the Coral Sea, near Cairns, Queensland (Australia) and in comparison to other Queensland wet tropics streams, it has a relatively large catchment area of about 219,000 ha. The catchment has high fish diversity, with at least 209 estuarine and freshwater species and the Barron River Estuary is a spawning and nursery ground for a variety of fish and prawn species and supports a wide range of commercial and recreational fish species. The variance and abundance of benthic macroinvertebrates can provide valuable information on the ecological condition of a river system. Overall, the macroinvertebrate populations indicated a relatively healthy system although nutrient enrichment may be causing degradation. Reserves such as existing Fish Habitat Areas and a proposed marine park will provide ongoing protection for coastal wetlands. A series of potential management issues for the catchment and future monitoring strategies are discussed

Kepemimpinan Kepala Sekolah dalam Meningkatkan Kualitas Pembelajaran Akuntansi Melalui Standar Sarana dan Prasarana di Sekolah Menengah Kejuruan Muhammadiyah 2 Karanganyar Tahun 2020/2021

This study aims to describe 1) the management of facilities and infrastructure at SMK Muhammadiyah 2 Karanganyar, 2) the role of the principal in improving the quality of accounting learning at SMK Muhammadiyah 2 Karanganyar. This research is a qualitative research with a ethnographic design. The subjects of this study were the principal, vice principal, accounting teacher and accounting students of SMK Muhammadiyah 2 Karanganyar. The data collection techniques used were interviews, observation and documentation. Researchers used source triangulation. Data analysis techniques used data collection, data reduction, data presentation and drawing conclusions. The results of this study indicate that 1) management of facilities and infrastructure is carried out starting from a) planning in the form of proposals submitted by teachers related to learning needs, b) procurement by checking availability and considering teacher opinions and available funds, c) use or utilization according to needs in learning and paying attention to its availability and feasibility, d) routine, periodic, and incidental maintenance, e) deletion of items that are damaged and cannot be reused. 2) The role of the principal in improving the quality of learning includes a) planning, including adjusting the needs for facilities and infrastructure as well as training or workshops for teachers, b) strategies, including optimizing the use of learning tools and media, implementing supervision, collaborating with the community, c) the learning process, by adjusting the material and infrastructure, d) constraints, including the lack of interest in learning by students and the facilities available at home

PERANCANGAN WEBSITE E-MARKETING PADA NOIR

PERANCANGAN WEBSITE E-MARKETING PADA NOIR

Investigating the efficacy of a proposed marine protected area for the Endangered humphead wrasse Cheilinus undulatus at a remote island group in Seychelles

The humphead wrasse Cheilinus undulatus is an iconic, ecologically important and Endangered fish species associated with coral reefs in the Indo-Pacific region. Due to its large size and complex life history characteristics, it is vulnerable to overfishing and has undergone substantial population declines in parts of its range. Knowledge of the species’ movement ecology is currently limited to only 2 previous studies, and very little is known about populations in the western Indian Ocean. The present study aimed to use passive acoustic telemetry to investigate the importance of a remote coral reef to a population of humphead wrasse in the Republic of Seychelles, and subsequently assess the efficacy of a proposed marine protected area at this location for protection of the species. Tagged fish (n = 20) exhibited persistent (\u3e500 d) site fidelity, with low dispersal distances (mean ± SD: 6.44 ± 4.0 km) and restricted core activity spaces (50% Brownian bridge kernel utilization density: 0.91 ± 0.61 km2). Additionally, the study site was home to a group of large (total length 97.9 ± 20.6 cm) and currently unexploited humphead wrasse that showed long-term predictable site fidelity and thus could be vulnerable to over-exploitation. The establishment of a proposed no-take marine protected area at the study site would encompass the core home range area of all tagged humphead wrasse and could effectively conserve this stronghold of Endangered fish to ensure the persistence of the species in Seychelles waters

Association between an AMH promoter polymorphism and serum AMH levels in PCOS patients

STUDY QUESTION: Do polymorphisms in the anti-Müllerian hormone (AMH) promoter have an effect on AMH levels in patients with polycystic ovary syndrome (PCOS)? SUMMARY ANSWER: We have identified a novel AMH promoter polymorphism rs10406324 that is associated with lower serum AMH levels and is suggested to play a role in the mechanism of regulation of AMH gene expression in women. WHAT IS KNOWN ALREADY: Follicle number is positively correlated with serum AMH levels, reflected by elevated AMH levels in women with PCOS. In addition, it is suggested that AMH production per follicle is higher in women with PCOS than in normo-ovulatory women, implying an altered regulation of AMH in PCOS. STUDY DESIGN, SIZE, DURATION: A discovery cohort of 655 PCOS women of Northern European ancestry and both an internal and external validation PCOS cohort (n = 458 and n = 321, respectively) were included in this study. Summary-level data of an AMH genome-wide association study meta-analysis including 7049 normo-ovulatory women was included as a control cohort. A genetic approach was taken through association analysis and in silico analysis of the associated variants in the AMH promoter. In vitro analysis was performed to investigate the functional mechanisms. PARTICIPANTS/MATERIALS, SETTING, METHODS: All common two-allelic single-nucleotide polymorphisms (SNPs) in the region Chr19:2 245 353–2 250 827 bp (Build 37) were selected for the analysis. Linear regression analyses were performed to determine the association between SNPs in the AMH promoter region and serum AMH levels. For the in silico analysis, the webtools ‘HaploReg’ v4.1 for ENCODE prediction weight matrices and ‘atSNP’ were used. In vitro analysis was performed using KK1 cells, a mouse granulosa cell line and COV434 cells, a human granulosa tumor cell line. Cells were transfected with the reference or the variant human AMH promoter reporter construct together with several transcription factors (TFs). Dual-Glo(®) Luciferase Assay was performed to measure the luciferase activity. MAIN RESULTS AND THE ROLE OF CHANCE: Polymorphism rs10406324 was significantly associated with serum AMH levels in all three PCOS cohorts. Carriers of the minor allele G had significantly lower log-transformed serum AMH levels compared to non-carriers (P = 8.58 × 10(−8), P = 1.35 × 10(−3) and P = 1.24 × 10(−3), respectively). This result was validated in a subsequent meta-analysis (P = 3.24 × 10(−12)). Interestingly, rs10406324 was not associated with follicle count, nor with other clinical traits. Also, in normo-ovulatory women, the minor allele of this variant was associated with lower serum AMH levels (P = 1.04 × 10(−5)). These findings suggest that polymorphism rs10406324 plays a role in the regulation of AMH expression, irrespective of clinical background. In silico analysis suggested a decreased binding affinity of the TFs steroidogenenic factor 1, estrogen-related receptor alpha and glucocorticoid receptor to the minor allele G variant, however in vitro analysis did not show a difference in promoter activity between the A and G allele. LIMITATIONS, REASONS FOR CAUTION: Functional analyses were performed in a mouse and a human granulosa cell line using an AMH promoter reporter construct. This may have limited assessment of the impact of the polymorphism on higher order chromatin structures. Human granulosa cells generated from induced pluripotent stem cells, combined with gene editing, may provide a method to elucidate the exact mechanism behind the decrease in serum AMH levels in carriers of the −210 G allele. We acknowledge that the lack of follicle number in the external validation and the control cohort is a limitation of the paper. Although we observed that the association between rs10406324 and AMH levels was independent of follicle number in our discovery and internal validation PCOS cohorts, we cannot fully rule out that the observed effects on serum AMH levels are, in part, caused by differences in follicle number. WIDER IMPLICATIONS OF THE FINDINGS: These results suggest that variations in serum AMH levels are not only caused by differences in follicle number but also by genetic factors. Therefore, the genetic context should be taken into consideration when assessing serum AMH levels in women. This may have clinical consequences when serum AMH levels are used as a marker for the polycystic ovarian morphology phenotype. STUDY FUNDING/COMPETING INTEREST(S): No external funding was used. J.S.E.L. has received consultancy fees from the following companies: Ferring, Roche Diagnostics and Ansh Labs and has received travel reimbursement from Ferring. J.A.V. has received royalties from AMH assays, paid to the institute/lab with no personal financial gain. The other authors declare no competing interests. TRIAL REGISTRATION NUMBER: N/A

Cognitive functioning in children with internalising, externalising and dysregulation problems: a population-based study

Psychiatric symptoms in childhood are closely related to neurocognitive deficits. However, it is unclear whether