Efficient Design of Integrated Underbody and Battery Pack for Battery Electric Vehicles

Climate change and the consequent more restrictive regulations are pushing the industry towards higher efficiency and lower emissions means of transport. The road transport sector is deeply affected, leading the main Original Equipment Manufacturers (OEMs) to start the transition to alternative powertrains, among which Battery Electric Vehicles (BEVs) are the faster-growing. The large, heavy, and safety-critical battery pack asks for a specific optimized solution for a lightweight and high-performance vehicle platform. In this research, a first benchmarking analysis is presented to study the current state-of-the-art for BEV underbody and battery pack designs. Following this investigation, a classification scheme was designed to allow easier comparison between different solutions, pointing out the most relevant characteristics and best design choices. Starting from the results of the benchmarking analysis, the study followed with a series of Finite Element Analyses (FEAs), on different simplified platform models, organized through a fractional factorial Design of Experiment (DOE). The responses of the experiments were the torsional stiffness and bending stiffness and first resonance mode, evaluated together with the mass of the system. These investigations revealed that the most influential factors for the analyzed performance outputs were the torque box and rocker rail internal structures, together with the material of the battery pack. Other parameters were less influential, but the study was still able to highlight the more favourable configurations. Through two additional analyses the battery pack was found to heavily affect the structure static stiffness, but both the battery pack and floor panels needed appropriate stiffening to avoid low-frequency resonance. The conducted analysis allowed the development of a linear regression model and the execution of a design optimization which delivered two different optimized solutions, showing good performance and high weight efficiency. After the discussion of the gathered results, validated design guidelines were created to provide a starting point for the development of future dedicated and integrated BEV underbodies and battery packs

Role of Circadian Clock on the Pathogenesis and Lifestyle Management in Non-Alcoholic Fatty Liver Disease

Several features of the modern lifestyle, such as weekly schedules or irregular daily eating patterns, have become major drivers of global health problems, including non-alcoholic fatty liver disease (NAFLD). Sleep is an essential component of human well-being, and it has been observed that when circadian rhythms are disrupted, or when sleep quality decreases, an individual’s overall health may worsen. In addition, the discrepancy between the circadian and social clock, due to weekly work/study schedules, is called social jetlag and has also been associated with adverse metabolic profiles. Current management of NAFLD is based on dietary intake and physical activity, with circadian preferences and other environmental factors also needing to be taken into account. In this regard, dietary approaches based on chrononutrition, such as intermittent fasting or time-restricted feeding, have proven to be useful in realigning lifestyle behaviors with circadian biological rhythms. However, more studies are needed to apply these dietary strategies in the treatment of these patients. In this review, we focus on the impact of circadian rhythms and the role of sleep patterns on the pathogenesis and development of NAFLD, as well as the consideration of chrononutrition for the precision nutrition management of patients with NAFLD

PDGF-BB Carried by Endothelial Cell-Derived Extracellular Vesicles Reduces Vascular Smooth Muscle Cell Apoptosis in Diabetes

Endothelial cell-derived extracellular vesicles (CD31EVs) constitute a new entity for therapeutic/prognostic purposes. The roles of CD31EVs as mediators of vascular smooth muscle cell (VSMC) dysfunction in type 2 diabetes (T2D) are investigated herein. We demonstrated that, unlike serum-derived extracellular vesicles in individuals without diabetes, those in individuals with diabetes (D CD31EVs) boosted apoptosis resistance of VSMCs cultured in hyperglycemic condition. Biochemical analysis revealed that this effect relies on changes in the balance between antiapoptotic and proapoptotic signals: increase of bcl-2 and decrease of bak/bax. D CD31EV cargo analysis demonstrated that D CD31EVs are enriched in membrane-bound platelet-derived growth factor-BB (mbPDGF-BB). Thus, we postulated that mbPDGF-BB transfer by D CD31EVs could account for VSMC resistance to apoptosis. By depleting CD31EVs of platelet-derived growth factor-BB (PDGF-BB) or blocking the PDGF receptor β on VSMCs, we demonstrated that mbPDGF-BB contributes to D CD31EV-mediated bak/bax and bcl-2 levels. Moreover, we found that bak expression is under the control of PDGF-BB-mediated microRNA (miR)-296-5p expression. In fact, while PDGF-BB treatment recapitulated D CD31EV-mediated antiapoptotic program and VSMC resistance to apoptosis, PDGF-BB-depleted CD31EVs failed. D CD31EVs also increased VSMC migration and recruitment to neovessels by means of PDGF-BB. Finally, we found that VSMCs, from human atherosclerotic arteries of individuals with T2D, express low bak/bax and high bcl-2 and miR-296-5p levels. This study identifies the mbPDGF-BB in D CD31EVs as a relevant mediator of diabetes-associated VSMC resistance to apoptosis