Additional file 2: of MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis

IL-1β gene expression in the peripheral white blood cells of patients with acute gouty arthritis (GA, n = 14) and of healthy control subjects (HC, n = 10). IL-1β mRNA expression was detected by quantitative real-time PCR. Each bar shows the mean ± SEM. (DOCX 48 kb

Additional file 1: Table S1. of MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis

Primer sequences used for the amplification of IL-1β 3′UTR. (DOCX 15 kb

Additional file 2: of MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis

IL-1β gene expression in the peripheral white blood cells of patients with acute gouty arthritis (GA, n = 14) and of healthy control subjects (HC, n = 10). IL-1β mRNA expression was detected by quantitative real-time PCR. Each bar shows the mean ± SEM. (DOCX 48 kb

Additional file 3: of MicroRNA-488 and -920 regulate the production of proinflammatory cytokines in acute gouty arthritis

THP-1 cells were transfected with 50 nM of miRNA mimics or negative control (scrambled) by using Lipofectamine RNAiMAX reagent. Expression of miRNAs was detected by quantitative real-time PCR. Each bar shows the mean ± SEM. (DOCX 31 kb

Carbohydrate–DNA Conjugation Enabled by Glycosyl Radicals Generated from Glycosyl Sulfinates

Herein, we report a method that enables the synthesis of carbohydrate–DNA conjugates by radical addition. Key to the success is the use of readily available, bench-stable, and unprotected glycosyl sulfinates as precursors to glycosyl radicals. The redox neutral reaction proceeds under mild and simple conditions and tolerates a broad substrate scope. A small library of carbohydrate–DNA conjugates was prepared