Mitochondrial respiration inhibition after exposure to UWB pulses as a possible mechanism of antitumor action

The respiration of isolated mice liver mitochondria after exposure to nanosecond UWB pulses (0.15 – 36 kV/cm, 0.6 – 1.0 GHz centre frequency, 3 – 20 ns pulse duration) has been investigated. The respiratory control (RC, the ratio of oxygen consumption) was estimated. The possibility of mitochondrial membrane electroporation was detected as the decrease in the electrical resistance, according to the β-dispersion of the electric current. The monotonous decrease of RC after 1000 UWB pulses from 0.15 kV/cm was observed, the ohmic resistance of mitochondria suspension was reduced. The obtained data indicate the inhibitory effect of UWB pulses on a state of irradiated mitochondria and its membrane

Study of the switching characteristics of gas S-diodes

In the study, the switching properties of avalanche GaAs S-diodes are investigated. We studied diode assemblies of various configurations. It is shown that when using two or more diodes connected in series, the edge of the voltage pulse can be sharpened to about 1 ns

Different sensitivity of normal and tumor cells to pulsed radiofrequency exposure

The effect of nanosecond radiofrequency pulses (nsRF) on tumor and normal cells has been studied. To determine the viability of cells, an MTT test was used, as well as a real time system for analyzing cell cultures-iCELLigence. It has been shown that ns RF pulses under certain combinations of operating conditions reduce cell proliferation of both tumor and normal cells. Double exposure to 1000 pulses leads to the most effective inhibition of tumor cell proliferation and was 40% after 5 days. Inhibition of the proliferative activity of normal cells was 10% and was maximum after 3 days, then cell growth resumed. The results obtained allow to consider ns RF pulses with different parameters as a promising effective factor for controlling cellular processes for biomedical purposes

Coherent summation of emission from relativistic Cherenkov sources as a way of production of extremely high-intensity microwave pulses

For relativistic Cherenkov devices, we investigate the process of high-power microwave pulse generation with its phase correlating to the sharp edge of an e-beam current pulse. Our theoretical consideration is referred to quasi-stationary and superradiative (SR) generation regimes when spontaneous emission of the e-beam edge serves as the seed for the development of further coherent oscillations. Phase correlation of the excited microwave pulses with the characteristics of the current pulse front and/or an initial external electromagnetic pulse has been additionally confirmed by particle-in-cell simulations. Pulse-to-pulse stability of the radiation phase within several percents of the oscillation period makes it possible to arrange multichannel schemes producing mutually coherent microwave pulses. In the experiments that have been carried out, the cathodes of independent generators were powered by identical accelerating pulses from strictly synchronized voltage modulators, or by splitting the pulse from a single powerful modulator. For the 2-ns regime with the power of each Ka-band backward-wave oscillator about 100 MW, we demonstrate quadratic growth of the power density in the interference maximum of the directional diagram. In a short pulse SR regime, with the peak power of 600 MW in a single channel, for a four-channel 2-D array, we attained a 16-fold radiation intensity gain

Mitochondrial respiration inhibition after exposure to UWB pulses as a possible mechanism of antitumor action

The respiration of isolated mice liver mitochondria after exposure to nanosecond UWB pulses (0.15 – 36 kV/cm, 0.6 – 1.0 GHz centre frequency, 3 – 20 ns pulse duration) has been investigated. The respiratory control (RC, the ratio of oxygen consumption) was estimated. The possibility of mitochondrial membrane electroporation was detected as the decrease in the electrical resistance, according to the β-dispersion of the electric current. The monotonous decrease of RC after 1000 UWB pulses from 0.15 kV/cm was observed, the ohmic resistance of mitochondria suspension was reduced. The obtained data indicate the inhibitory effect of UWB pulses on a state of irradiated mitochondria and its membrane