21 research outputs found
One-Pot Green Synthesis and Bioapplication ofl-Arginine-Capped Superparamagnetic Fe3O4 Nanoparticles
Water-solublel-arginine-capped Fe3O4 nanoparticles were synthesized using a one-pot and green method. Nontoxic, renewable and inexpensive reagents including FeCl3,l-arginine, glycerol and water were chosen as raw materials. Fe3O4 nanoparticles show different dispersive states in acidic and alkaline solutions for the two distinct forms of surface bindingl-arginine. Powder X-ray diffraction and X-ray photoelectron spectroscopy were used to identify the structure of Fe3O4 nanocrystals. The products behave like superparamagnetism at room temperature with saturation magnetization of 49.9 emu g−1 and negligible remanence or coercivity. In the presence of 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride, the anti-chloramphenicol monoclonal antibodies were connected to thel-arginine-capped magnetite nanoparticles. The as-prepared conjugates could be used in immunomagnetic assay
Transformation of Framework of Triple Cubane- Type Rh-Mo Oxide Cluster by Using Mercaptans: Dynamic Behavior of Linear-Type TetranuclearComplexes in Solution
Transformation of Framework of Triple Cubane- Type Rh-Mo Oxide Cluster by Using Mercaptans: Dynamic Behavior of Linear-Type TetranuclearComplexes in Solution
Facile Metathetical Exchange between Carbon Dioxide and the Divalent Group 14 Bisamides M[N(SiMe 3
Preparation of Anti-Tetracycline Antibodies and Development of an Indirect Heterologous Competitive Enzyme-Linked Immunosorbent Assay to Detect Residues of Tetracycline in Milk
Reversing tumor to “Hot”:A NIR light-triggered carrier-free nanoplatform for enhanced tumor penetration and photo-induced immunotherapy
Immunogenic cell death (ICD) process associated with phototherapy is a promising strategy to inhibit tumor growth and metastasis. To facilitate deep tumor-penetrating photo-immunotherapy, we herein report a near-infrared (NIR) light-triggered carrier-free nanoplatform (IR837) loaded with indocyanine green (ICG) and immune adjuvant R837. The IR837 nanoparticles were prepared as carrier-free nanoassembly and showed good photostability. With the thermal-responsive polydopamine as the shell, the IR837 (∼78 nm) can efficiently disassociate into smaller size (∼10 nm) under NIR irradiation. Through intravenous injection, IR837 displays prolonged blood circulation and turns into the smaller-sized nanoaggregates to deeply penetrate in the core sites of tumors. The ICD process is then induced and results in dendritic cells (DCs) maturation to initiate the immune response. Eventually, the “cold” tumor would be reversed into the “hot” tumor, promoting the immunotherapeutic outcomes against in-situ and distal tumors under mild temperature (45 °C). In summary, our research provides a prospective intelligent nanodelivery system to perform efficient photothermal immunotherapy against primary and distal tumor growth with hopefully low side effect
Synergetic Pyroptosis with Apoptosis Improving Phototherapy of Mitochondria-Targeted Cyanines with Superior Photostability
Pyroptosis has been reported to improve the antitumor
effect by
evoking a more intense immune response and a therapeutic effect. For
phototherapy, several photosensitizers have been found to initiate
pyroptosis. However, the effect of pyroptosis associated with apoptosis
in enhancing the antitumor therapy needs sufficient characterization,
especially under long-term treatment. As a NIR photosensitizer, heptamethine
cyanines have been discovered for anticancer phototherapy for deep
tissue penetration and inherent tumor-targeted capability. However,
they are not quite stable for long-term performance. To investigate
the effect of pyroptosis along with apoptosis on the anticancer immune
responses and phototherapy, here, we chemically modulate the cyanine
IR780 to regulate hydrophobicity, stability, and intracellular targeting.
Two photosensitizers, T780T-TPP and T780T-TPP-C12, were
finally optimized and showed excellent photostability with high photothermal
conversion efficiency. Although the cellular uptake of the two molecules
was both mediated by OATP transporters, T780T-TPP induced tumor cell
death via pyroptosis and apoptosis and accumulated in tumor accumulation,
while T780T-TPP-C12 was prone to accumulate in the liver.
Ultimately, via one injection-multiple irradiation treatment protocol,
T780T-TPP displayed a significant antitumor effect, even against the
growth of large tumors (200 mm3)
Establishment of Enhanced Chemiluminescent Immunoassay Formats for Stanozolol Detection in animal-derived foodstuffs and Other Matrices
Chemiluminescence Immunoassay for S‑Adenosylhomocysteine Detection and Its Application in DNA Methyltransferase Activity Evaluation and Inhibitors Screening
Aberrant
methylation by DNA transferase is associated with cancer
initiation and progression. For high-throughput screening of DNA methyltransferase
(MTase) activity and its inhibitors, a novel chemiluminescence immunoassay
(CLIA) was established to detect S-adenosylhomocysteine (SAH), the
product of S-adenosylmethionine (SAM) transmethylation reactions.
We synthesized two kinds of immunogens for SAH and characterized the
polyclonal antibodies in each group. The antibody with higher titer
was used to develop a competitive CLIA for SAH, in which SAH in samples
would compete with SAH coated on microplate in binding with SAH antibodies.
Successively, horseradish peroxidase labeled goat antirabbit IgG (HRP-IgG)
was conjugated with SAH antibodies on the microplate. In substrate
solution containing luminol and H<sub>2</sub>O<sub>2</sub>, HRP-IgG
catalyzed luminol oxidation by H<sub>2</sub>O<sub>2</sub>, generating
a high chemiluminescence signal. The method could detect as low as
9.8 ng mL<sup>–1</sup> SAH with little cross-reaction (3.8%)
to SAM. Since higher DNA MTase activity leads to more production of
SAH, a correlation between the chemiluminescence intensity and DNA
MTase activity was obtained in the range from 0.1 to 8.0 U/mL of DNA
MTase. The inhibition study showed that, in the presence of SAM as
methyl donor, Lomeguatrib, 5-Azacytidine, and 5-Aza-2′-deoxycytidine
could inhibit the DNA MTase activity with IC<sub>50</sub> values of
40.57 nM, 2.26 μM, and 0.48 μM, respectively. These results
are consistent with the published studies. The proposed assay does
not depend on recognizing methylated cytosines in oligonucleotides
(methyl acceptor) and showed the potential as an accessible platform
for sensitive detection of DNA MTase activity and screening its inhibitors