Effect of a combination of enzymes on the fundamental rheological behavior of bread dough enriched with resistant starch

The effect of three enzymes on the fundamental rheological parameters of bread dough with high content of resistant starch (RS) was studied. The RS was added as an alternative to increase the fiber ingestion while the enzymes, to overcome the gluten dilution. Optimum dough was formulated with partial substitution of wheat flour by RS (12.5 g/100 g) and enzymes transglutaminase (4 mg/100 g), glucose oxidase (2.5 mg/100 g) and xylanase (0.5 mg/100 g). Dough produced with RS and without enzymes was considered as control and dough without RS or enzymes was considered as regular for comparison. Fundamental rheological parameters were obtained from uniaxial extension, biaxial extension and oscillatory tests. Also, starch gelatinization and retrogradation were studied by differential scanning calorimetry. The partial replacement of WF by RS resulted in less extensible dough, whereas the addition of enzymes increased the strain hardening index allowing higher dough expansion. The addition of enzymes reduced the elastic modulus resulting in a behavior similar to the regular dough. RS was not gelatinized during baking, hence it can be considered as dietetic fiber. Wheat starch retrogradation after 7 days of storage was observed, indicating bread aging.Fil: Altuna, Luz. Universidade de Sao Paulo; BrasilFil: Ribotta, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Tadini, Carmen C.. Universidade de Sao Paulo; Brasi

Chemical composition and physical properties of sorghum flour prepared from different sorghum hybrids grown in Argentina

This work analyzed the physical, chemical, and thermal properties of sorghum flour and the relationships among these, in order to evaluate its suitability for the development of food products. Sorghum flour was obtained through roller dry milling from 20 commercial hybrids grown in Argentina with the average chemical composition of the samples being: 0.68% ash, 3.67% fat, 12.21% protein, 83.45% total carbohydrates, 79.77% starch (amylose 26.6%), and 34.9 mg of tannic acid per 100 g of flour. A high degree of variability among evaluated properties was found, particularly in the pasting properties peak viscosity (2809–5184 mPa/s), breakdown (1169–3170 mPa/s), and final viscosity (3030–4401 mPa/s) with onset temperature (To) and gelatinization enthalpy (ΔH) varying between 66.8 and 72.6°C, and 5.38 and 8.48 J/g, respectively. A principal component analysis demonstrated that the grain color did not influence the chemical composition of the flour. Cluster analysis permitted the separation of flour into three different groups with different thermal and physicochemical characteristics, and enabled the selection of hybrids. Thus, sorghum flour is a versatile ingredient and can be used in several food and non-food applications.Fil: Palavecino, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Penci, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Calderón-Domínguez, Georgina. Instituto Politécnico Nacional; MéxicoFil: Ribotta, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentin

Effect of planetary ball milling on physicochemical and morphological properties of sorghum flour

Planetary ball milling was applied to white sorghum flour with the aim of modifying its thermal, structural and morphological properties. Median particle size decreased from 57.2 μm a 20.8 μm with increasing milling energy. Particle size-energy models indicated that only part of the energy was used for developing new surfaces. Abrasion of starch granules could be observed by SEM and the increment of damaged starch levels. Gelatinization temperatures measured by DSC were not affected by the process (Tp average 73.4 ± 0.4 °C); yet, gelatinization enthalpy (ΔH) and crystallinity degree (determined by WAXS) decreased with increasing milling energy from 5.54 J/g and 28% to 2.98 J/g and 17.0%, respectively. Then, some pasting parameters significantly changed: final viscosity (from 3947 to 3535 cP) and, consequently, setback (from 2339 to 1896 cP). Planetary ball milling significantly changed the functional properties of sorghum flour and suggested that this method is an alternative to widen sorghum flour food applications.Fil: Palavecino, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Penci, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Ribotta, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina. Universidad Nacional de Córdoba. Secretaría de Ciencia y Tecnología. Instituto Superior de Investigación, Desarrollo y Servicio de Alimentos; Argentin

Impact of chemical modifications in pilot-scale isolated sorghum starch and commercial cassava starch

The aim of this work was to evaluate the impact of different chemical modification methods on starch from different botanical sources. Brown and white sorghum starch was isolated throughout a time saving process and avoiding the use of dangerous compounds. Brown and white sorghum and commercial cassava starch were treated using acetic acid, acetic anhydride and octanoyl chloride. Its chemical, morphological and thermal characterization was afterwards carried out. The modifications reduced amylose content and increased damaged starch. Both acetylation and acid treatment produced no significant changes in the size and shape of granules; yet, they increased superficial pores. SEM observations supported the results, indicating that octanoyl modification causes a partial destruction of the granule structure. Thus, particle size distribution changes significantly. Crystallinity degree decreased with all the modifications. However, the effect was more pronounced in octanoyl esterification. In general, modifications increase the water absorption of the native starch but no considerable effect was found over oil absorption, and gelatinization parameters were significantly altered as a result of modifications. Starch from these subtilized sources was successfully modified, which could arouse interest in its industrial production.Fil: Palavecino, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Penci, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Ribotta, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentina. Instituto Superior de Investigación, Desarrollo y Servicios en Alimentos, SeCyT; Argentin

Effects of enzymatic modification of soybean protein on the pasting and rheological profile of starch–protein systems

ABSTRACT Reformulation of traditional food systems to introduce new ingredients may change their structure and perceived texture. Interactions between proteins and starch during processing can markedly influence starch gel network structure and rheological profile. The present work aimed to study the effects of soybean protein and the products of enzymatic modification on the pasting and rheological profile of corn and cassava starch. The behaviour of those protein enriched gels during storage was also assessed. Soybean protein isolate (SPI) was incubated with endopeptidase (AL) or food grade microbial transglutaminase (TG). Pasting and rheological behaviour, water retention capacity and structure of protein-and hydrolysed protein-starch gels were analyzed. Protein incorporation increased the viscosity of starch suspension during and after heating. SPImodified proteins increased peak viscosity. Only the structural modifications brought by TG on SPI increased the final viscosity during starch pasting and the storage modulus (G′). This modulus (G′) of the gelled systems decreased with the addition of AL-treated protein isolate. Light and fluorescence microscopy showed that SPI formed a continuous phase, like a network, in the gelled system. Different network structures and rheological properties can be obtained when SPI are modified by protease and transglutaminase enzymes, which may be very useful for designing new food products

Chia (Salvia hispanica L.) oil stability: Study of the effect of natural antioxidants

The chia seed (Salvia hispanica L.) is globally popular and valued for its nutritional and health attributes. Chia oil is mainly composed of triglycerides, in which polyunsaturated fatty acids (PUFAs, linoleic and α-linolenic acids) are found in high amounts. Although it seems evident that such fatty acid composition is favorable from a nutritional point of view, a higher content of linoleic and linolenic acids results in poorer oxidative stability and shorter shelf life of the oil. The aim of this study was to evaluate the combined effects of the storage condition (300 days under fluorescent light - 800 Lux - or in the dark, both at room temperature) with the addition of natural antioxidants (rosemary extract, RE; tocopherol, TOC; ascorbyl palmitate, AP). In the dark, the combined addition of AP and TOC significantly reduced lipid oxidation and improved oil shelf life. Moreover, this combination maintained an acceptable quality of at least up to 300 storage days. Results from this work highlight the influence of illumination condition on chia oil oxidative stability, suggesting that this oil should be stored in containers with light-barrier properties, and probably added to the antioxidants examined in the current study.Fil: Bodoira, Romina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Penci, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Ribotta, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Martinez, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentin

Estudio de las transformaciones fisico-químicas que se producen durante el procesamiento de masas congeladas

Objetivo general: • Estudiar las transformaciones fisicoquímicas que se producen durante la congelación y el almacenamiento en estado congelado de las masas para elaborar pan. Objetivos particulares: • Estudiar el efecto del proceso de congelación y almacenamiento en estado congelado sobre la calidad del pan. • Determinar los cambios físicoquímicos y funcionales sufridos por las proteínas durante la congelación y el almacenamiento de la masa de pan. • Determinar los cambios físicoquímicos y funcionales sufridos por el almidón durante la congelación y el almacenamiento de la masa de pan y el envejecimiento del pan. • Estudiar el efecto del proceso de congelación de las levaduras sobre su viabilidad, capacidad de producción de gas y las sustancias liberadas por las células. • Analizar la influencia del proceso de congelación y el almacenamiento a -18°C sobre el comportamiento reológico de las masas. • Estudiar el efecto del proceso de congelación y almacenamiento sobre la microestructura de la masa. • Estudiar el efecto del agregado de aditivos a las masas sobre el proceso de congelación y almacenamiento a -18°C. • Estudiar el efecto de la precocción de la masa y su almacenamiento sobre la calidad del pan.Tesis digitalizada en SEDICI gracias a la Biblioteca Central de la Facultad de Ciencias Exactas (UNLP).Facultad de Ciencias Exacta

Study of the preparation process and variation of wall components in chia (Salvia hispanica L.) oil microencapsulation

A study of the microencapsulation process of omega-3 rich oil extracted from chia (Salvia hispanica L.) seeds was carried out, which included a comparative analysis of the microcapsules obtained by the spray- and freeze-drying methods using isolated soy proteins and maltodextrin as wall materials at different proportions. Color characterization of the obtained powders was performed and revealed a darker and yellower appearance of the freeze-dried samples compared with the spray-dried ones. Moisture content was measured for each sample and all presented values around 3.5%. The SEM micrographs revealed that spray-dried microcapsules can occur individually or may form clusters or aggregates, with the particles exhibiting a size range varying from 4 to 10 µm. Encapsulation efficiency was measured, with no significant differences found between drying methods or on varying the proportion of wall components (all samples presented values of aproximately 60%). The oxidative stability of microencapsulated oils under accelerated oxidative conditions revealed protection factors 2-fold higher for all samples. In addition, microencapsulated oil stored under 25 ºC revealed lower hydroperoxide values than those of unencapsulated oil throughout the whole storage test. Finally, oil encapsulated in SPI microcapsules also showed lower HPV values than that of the acceptable limit for virgin and cold-pressed vegetable oils (15 meq. O2/kg oil) during the storage test, while unencapsulated oil attained the acceptable limit in 62 days. This result represents an increase in the time of the oil shelf life of between 30 and 48%.Fil: González, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Martinez, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Paredes, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Leon, Alberto Edel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Ribotta, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentin

Estudio de las transformaciones fisico-químicas que se producen durante el procesamiento de masas congeladas

Objetivo general: • Estudiar las transformaciones fisicoquímicas que se producen durante la congelación y el almacenamiento en estado congelado de las masas para elaborar pan. Objetivos particulares: • Estudiar el efecto del proceso de congelación y almacenamiento en estado congelado sobre la calidad del pan. • Determinar los cambios físicoquímicos y funcionales sufridos por las proteínas durante la congelación y el almacenamiento de la masa de pan. • Determinar los cambios físicoquímicos y funcionales sufridos por el almidón durante la congelación y el almacenamiento de la masa de pan y el envejecimiento del pan. • Estudiar el efecto del proceso de congelación de las levaduras sobre su viabilidad, capacidad de producción de gas y las sustancias liberadas por las células. • Analizar la influencia del proceso de congelación y el almacenamiento a -18°C sobre el comportamiento reológico de las masas. • Estudiar el efecto del proceso de congelación y almacenamiento sobre la microestructura de la masa. • Estudiar el efecto del agregado de aditivos a las masas sobre el proceso de congelación y almacenamiento a -18°C. • Estudiar el efecto de la precocción de la masa y su almacenamiento sobre la calidad del pan.Tesis digitalizada en SEDICI gracias a la Biblioteca Central de la Facultad de Ciencias Exactas (UNLP).Facultad de Ciencias Exacta

Differential scanning calorimetry (DSC) studies on the thermal properties of peanut proteins

Studies related to the functional and thermal properties of peanut proteins are limited if compared with other vegetable protein sources. The aim of this work was to study the thermal denaturation of peanut protein isolates (PPI) by DSC. The thermal profile of PPI showed two endothermic peaks (assigned to denaturation of arachin and conarachin fractions). The thermal stability of arachin and conarachin increased when water content decreased, and a critical water level was found for both fractions. The effect of protein denaturants was studied. Low contents of urea stabilized protein fractions, but lower T d values were found with increasing concentrations. δH values of arachin were affected by urea. SDS affected δH values and thermal stability of conarachin; the arachin fraction showed higher resistance to SDS-induced denaturation. DTT addition did not affect conarachin stability, although enthalpy values decreased significantly. On the other hand, arachin was greatly affected by DTT. In summary, thermal denaturation parameters of PPI were sensitive to water content, indicating that polar groups of arachin and conarachin contribute to structure stabilization. Urea addition mainly affected the structure of the arachin fraction, which was attributed to its higher surface hydrophobicity. Results obtained from SDS and DTT suggest that hydrophobic interactions and disulfide bonds play an important role in structure maintenance of arachin and conarachin.Fil: Colombo, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Ribotta, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; ArgentinaFil: Leon, Alberto Edel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Ciencia y Tecnología de Alimentos Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Ciencia y Tecnología de Alimentos Córdoba; Argentin