3 research outputs found
l-Ergothioneine Protects Skin Cells against UV-Induced DamageâA Preliminary Study
Many changes related to aging at the cellular level may be due to the physiological condition of mitochondria. One of the most common types of damage of mtDNA is the so-called âcommon deletionâ referring to a deletion of 4977 base pairs. In the skin cells this phenomenon probably is caused by oxidative damage of mtDNA induced by UV. The present study was aimed at evaluating the effect of the antioxidant l-ergothioneine on UV-induced damage in skin cells. The effect of l-ergothioneine on the reduced glutathione level was studied. The presence of the âcommon deletionâ in human fibroblasts irradiated with UVA and treated with l-ergothioneine was evaluated by a polymerase chain reaction. We have demonstrated that l-ergothioneine enhanced the level of reduced glutathione and protected cells from the induction of a photoaging-associated mtDNA âcommon deletionâ. In view of our results, l-ergothioneine could be an effective skin care and anti-photoaging ingredient
Toward selective detection of reactive oxygen and nitrogen species with the use of fluorogenic probes â Limitations, progress, and perspectives
International audienceOver the last 40 years, there has been tremendous progress in understanding the biological reactions ofreactive oxygen species (ROS) and reactive nitrogen species (RNS). It is widely accepted that the generationof ROS and RNS is involved in physiological and pathophysiological processes. To understand the role of ROSand RNS in a variety of pathologies, the specific detection of ROS and RNS is fundamental. Unfortunately,the intracellular detection and quantitation of ROS and RNS remains a challenge. In this short review, wehave focused on the mechanistic and quantitative aspects of their detection with the use of selectedfluorogenic probes. The challenges, limitations and perspectives of these methods are discussed
Designing of Biodegradable and Biocompatible Release and Delivery Systems of Selected Antioxidants Used in Cosmetology
Conjugates of antioxidants p-anisic
(p-AA) and vanillic (VA) acids
with nontoxic, biocompatible, and biodegradedable oligo-<i>(R,S)</i>-(3-hydoxybutyrate) carrier were synthesized, and their structural
and biological characterization was performed. The molecular structure
of the bioconjugates, in which antioxidants are covalently bonded
with oligoÂ(3-hydroxybutyrate) (OHB) chains, has been proven by mass
spectrometry supported by NMR. The bioconjugate hydrolytic degradation
studies allowed gaining thorough insight into the hydrolysis process
and confirmed the release of p-AA and VA. In vitro studies demonstrated
that all of the conjugates studied were well tolerated by KB and HaCaT
cell lines, as they had no marked cytotoxicity, while conjugates with
a relatively short OHB carrier are optimal to support keratinocyte
function. The preliminary study of the biological activity confirmed
the protective effect of VAâOHB conjugates against H<sub>2</sub>O<sub>2</sub>-induced lipid peroxidation in human keratinocytes (HaCaT).
It was also demonstrated that the selected bioconjugates can penetrate
all layers of the skin, which shows their functionality and opens
up their potential application in cosmetology