P53 tumour-suppressor gene mutations are mainly localised on exon 7 in human primary and metastatic prostate cancer.

Mutations in the p53 tumour-suppressor gene are among the most common genetic alterations in human cancers. In the present study we analysed the mutations in the p53 tumor-suppressor gene in 25 primary and 20 metastatic human prostate cancer specimens. DNA extracted from the paraffin-embedded sections was amplified by hot-start polymerase chain reaction, and p53 gene mutations in the conserved mid-region (exons 4-9) were examined using single-strand conformation polymorphism (SSCP) analysis and immunohistochemistry. In the present study, we used a novel hot-start PCR-SSCP technique using DNA Taq polymerase antibody, which eliminates primer-dimers and non-specific products. Because of this new technique, the results of PCR-SSCP showed very high resolution. Polymerase chain reaction products were sequenced directly for point mutations for the p53 gene. Mutations were found in 2 out of 25 primary prostate cancers (8%) and 4 out of 20 metastatic cancers (20%). Mutations were observed exclusively in exon 7 and not in exons 4, 5, 6, 8 or 9. Nuclear accumulation of p53 protein, determined by immunohistochemistry, correlated with the degree of metastasis in prostatic cancer

Business IT strategy in action : case study of #PUPRU

Predicted key business IT trends through to 2020 acknowledge there will be increased adoption of disruptive technologies that will impact within business contexts (Gartner (1), 2015). These include moves to small-screen marketing, rising value of big data intelligence and increased use of social systems. However, IT penetration and literacy gaps between advanced and emerging economies, as well as social and organisational issues associated with technological implementation of business strategy, provide challenges to adoption of these trends. Strategy As Practice (SAP) presents a potential solution. It is concerned with the practice of strategising, including both the formulation of strategy and implementation that delivers strategic renewal and change. Through the “doing of strategy”, organisations not only gain an insight into strategic management from a theoretical level, but also can focus on micro-level social activities, process and practices that characterise both organisational strategy and strategising. In this paper, the Pop-Up Research Unit (#PUPRU), a newly-established mobile research centre in the Salford Business School (SBS) will be discussed. It aims to adopt a SAP approach to inform business strategy. Three disruptive technologies - Beacon, Raspberry Pi and 3D printer - available as part of #PUPRU will be examined. Future experiments in various business scenarios and live projects evaluating how these digital disrupters can be employed will also be considered

Towards Work-Efficient Parallel Parameterized Algorithms

Parallel parameterized complexity theory studies how fixed-parameter tractable (fpt) problems can be solved in parallel. Previous theoretical work focused on parallel algorithms that are very fast in principle, but did not take into account that when we only have a small number of processors (between 2 and, say, 1024), it is more important that the parallel algorithms are work-efficient. In the present paper we investigate how work-efficient fpt algorithms can be designed. We review standard methods from fpt theory, like kernelization, search trees, and interleaving, and prove trade-offs for them between work efficiency and runtime improvements. This results in a toolbox for developing work-efficient parallel fpt algorithms.Comment: Prior full version of the paper that will appear in Proceedings of the 13th International Conference and Workshops on Algorithms and Computation (WALCOM 2019), February 27 - March 02, 2019, Guwahati, India. The final authenticated version is available online at https://doi.org/10.1007/978-3-030-10564-8_2

SL(2,R)SL(2,R) symmetry and quasi-normal modes in the BTZ black hole

With the help of two new intrinsic tensor fields associated with the $SL(2,R)$ quadratic Casimir of Killing fields, we uncover the $SL(2,R)$ symmetry satisfied by the solutions to the equations of motion for various fields in the BTZ black hole in a uniform way by performing tensor and spinor analysis without resorting to any specific coordinate system. Then with the standard algebraic method developed recently, we determine the quasi-normal modes for various fields in the BTZ black hole. As a result, the quasi-normal modes are given by the infinite tower of descendants of the chiral highest weight mode, which is in good agreement with the previous analytic result obtained by exactly solving equations of motion instead.Comment: JHEP style, 1+13 pages, version to appear in JHE

Cooperative contractility: the role of stress fibres in the regulation of cell-cell junctions.

We present simulations of cell-cell adhesion as reported in a recent study [Liu et al., 2010, PNAS, 107(22), 9944-9] for two cells seeded on an array of micro-posts. The micro-post array allows for the measurement of forces exerted by the cell and these show that the cell-cell tugging stress is a constant and independent of the cell-cell junction area. In the current study, we demonstrate that a material model which includes the underlying cellular processes of stress fibre contractility and adhesion formation can capture these results. The simulations explain the experimentally observed phenomena whereby the cell-cell junction forces increase with junction size but the tractions exerted by the cell on the micro-post array are independent of the junction size. Further simulations on different types of micro-post arrays and cell phenotypes are presented as a guide to future experiments.WR and PMcG acknowledge Science Foundation Ireland grant 10/RFP/ENM2960 and Short Term Travel Fellowship (STTF 11).This is the accepted manuscript. The final version is available from Elsevier at http://www.sciencedirect.com/science/article/pii/S0021929014006137

pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis

Background The ethanol-producing bacterium Zymomonas mobilis has attracted considerable scientific and commercial interest due to its exceptional physiological properties. Shuttle vectors derived from native plasmids have previously been successfully used for heterologous gene expression in this bacterium for a variety of purposes, most notably for metabolic engineering applications. Results A quantitative PCR (qPCR) approach was used to determine the copy numbers of two endogenous double stranded DNA plasmids: pZMO1A (1,647 bp) and pZMO7 (pZA1003; 4,551 bp) within the NCIMB 11163 strain of Z. mobilis. Data indicated pZMO1A and pZMO7 were present at ca. 3-5 and ca. 1-2 copies per cell, respectively. A ca. 1,900 bp fragment from plasmid pZMO7 was used to construct two Escherichia coli - Z. mobilis shuttle vectors (pZ7C and pZ7-184). The intracellular stabilities and copy numbers of pZ7C and pZ7-184 were characterized within the NCIMB 11163, ATCC 29191 and (ATCC 10988-derived) CU1 Rif2 strains of Z. mobilis. Both shuttle vectors could be stably maintained within the ATCC 29191 strain (ca. 20-40 copies per cell), and the CU1 Rif2 strain (ca. 2-3 copies per cell), for more than 50 generations in the absence of an antibiotic selectable marker. A selectable marker was required for shuttle vector maintenance in the parental NCIMB 11163 strain; most probably due to competition for replication with the endogenous pZMO7 plasmid molecules. N-terminal glutathione S-transferase (GST)-fusions of four endogenous proteins, namely the acyl-carrier protein (AcpP); 2-dehydro-3-deoxyphosphooctonate aldolase (KdsA); DNA polymerase III chi subunit (HolC); and the RNA chaperone protein Hfq; were successfully expressed from pZ7C-derived shuttle vectors, and their protein-protein binding interactions were analyzed in Z. mobilis ATCC 29191. Using this approach, proteins that co-purified with AcpP and KdsA were identified. Conclusions We show that a shuttle vector-based protein affinity 'pull-down' approach can be used to probe protein interaction networks in Z. mobilis cells. Our results demonstrate that protein expression plasmids derived from pZMO7 have significant potential for use in future biological or biotechnological applications within Z. mobilis.published_or_final_versio

Timed Implementation Relations for the Distributed Test Architecture

In order to test systems that have physically distributed interfaces, called ports, we might use a distributed approach in which there is a separate tester at each port. If the testers do not synchronise during testing then we cannot always determine the relative order of events observed at different ports and this leads to new notions of correctness that have been described using corresponding implementation relations. We study the situation in which each tester has a local clock and timestamps its observations. If we know nothing about how the local clocks relate then this does not affect the implementation relation while if the local clocks agree exactly then we can reconstruct the sequence of observations made. In practice, however, we are likely to be between these extremes: the local clocks will not agree exactly but we have some information regarding how they can differ. We start by assuming that a local tester interacts synchronously with the corresponding port of the system under test and then extend this to the case where communications can be asynchronous, considering both the first-in-first-out (FIFO) case and the non-FIFO case. The new implementation relations are stronger than implementation relations for distributed testing that do not use timestamps but still reflect the distributed nature of observations. This paper explores these alternatives and derives corresponding implementation relations

A novel algorithm for dynamic student profile adaptation based on learning styles

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.E-learning recommendation systems are used to enhance student performance and knowledge by providing tailor- made services based on the students’ preferences and learning styles, which are typically stored in student profiles. For such systems to remain effective, the profiles need to be able to adapt and reflect the students’ changing behaviour. In this paper, we introduce new algorithms that are designed to track student learning behaviour patterns, capture their learning styles, and maintain dynamic student profiles within a recommendation system (RS). This paper also proposes a new method to extract features that characterise student behaviour to identify students’ learning styles with respect to the Felder-Silverman learning style model (FSLSM). In order to test the efficiency of the proposed algorithm, we present a series of experiments that use a dataset of real students to demonstrate how our proposed algorithm can effectively model a dynamic student profile and adapt to different student learning behaviour. The results revealed that the students could effectively increase their learning efficiency and quality for the courses when the learning styles are identified, and proper recommendations are made by using our method