Histopathological Analysis of Skeletal Muscle Biopsy of Patient with Peripheral Arterial Disease before and after Peripheral Blood Stem Cells Intramuscular Injection

© 2016, Springer Science+Business Media New York.Peripheral arterial diseases are characterized by a progressing tissue ischemia which results in the invalidization of patients. The aim of our research was to study the morphological effects of autologous peripheral blood stem cells intramuscular injection into patients with peripheral arterial disease. Peripheral blood stem cells were transplanted intramuscularly into a 48-year-old male patient with peripheral arterial disease stage IIb by Fontaine. The biopsies of his gastrocnemius muscle were taken before the stem cells were transplanted and 3 months after transplantation. These biopsies were stained with H&E and also with antibodies against CD34, myogenin, caspase 3, and bcl-2. Immunohistochemical study results showed an increase of capillary density of 32.7 % (P = 0.005). In muscular biopsies obtained before therapy, we identified single myogenin+ myosatellite cells, while 3 months after transplantation we detected the presence of cells with myogenin-positive nuclei and multinucleated myotubes. We also observed the formation of young myogenin+ muscle fibers with central nuclei. There was no significant difference in the expression of caspase-3 before transplantation and 3 months after transplantation. An increased number of bcl-2+ myosatellite cells, myotubes, and muscle fibers were detected after transplantation. The patient’s ankle-brachial index increased by 13.56 % (0.59 before and 0.67 3 months post transplantation). The patient’s pain-free walking distance by 89.97 % (from 59.56 to 113.77 m). Control arteriograms showed the formation of new collaterals. Transplanted autologous peripheral blood stem cells stimulated the formation of new capillaries, the activation of myosatellite cells and bcl-2 expression in muscle fibers

One-dimensional dynamics of domain walls in two-layer ferromagnet structure with different parameters of magnetic anisotropy and exchange

a b s t r a c t One-dimensional non-linear dynamics of domain walls (DW) under the influence of external constant magnetic field in a two-layer ferromagnet with different values of magnetic anisotropy and exchange parameters in the layers is theoretically studied in the article. Using analytical methods a motion equation for the DW centre coordinate, its stationary velocity after transition from one layer to another and its minimum velocity necessary for DW transition from one layer to another are found. It is shown that for the case of small defects, the results obtained analytically are well coordinated with the numerical ones

SO2 removal from air with dielectric barrier discharges

Este artigo tem como objetivo examinar os usos e os efeitos da passagem pelo sistema de ensino, focalizando estratégias desenvolvidas por famílias de dirigentes paulistas para controlar a educação escolar de suas filhas, em uma cidade em acelerado processo de urbanização, crescimento demográfico vertiginoso e de industrialização crescente como era São Paulo nas décadas de 1950 e 1960. Constituiu em objeto de estudo as trajetórias socioprofissionais de um grupo de mulheres, ex-alunas de três escolas católicas da cidade, para interrogar-se sobre a gênese dos investimentos femininos no mundo social. Os procedimentos de pesquisa incluiram uma pesquisa de campo, a aplicação de questionários e a realização de entrevistas biográficas com ex-professoras, diretoras das escolas e ex-alunas. Os resultados sugerem que o investimento diferenciado dessas mulheres sobre a esfera profissional está relacionado com a estrutura de patrimônio das famílias, as formas de estruturar a experiência escolar das meninas e os padrões de alianças matrimoniais realizadas por esse grupo de mulheres.This research studied the social and professional experience of a group of 82 women born between 1940 and 1950, coming from high income groups from São Paulo city, all ex-students of three Catholic women's colleges. The main objective was to identify and compare the forms of socialization to which they were exposed in their socioprofessional training. Research methodology included fieldwork, questionnaires and biographical interviews with former lecturers, school directors and past students. The study's findings enabled a better understanding of the mechanisms through which socialization processes determine the different dispositions characterizing the former students of a particular teaching system. They also helped show how the outcomes of this 'primary' socialization interact with later experiences, modifying, reconstituting or reproducing past social patterns and expectations

Western Star, 1919-12-10

The Western Star began publication on Newfoundland's west coast on 4 April 1900, appearing weekly with brief semiweekly periods up to 1952, when it became a daily. As of 17 April 2019 it continues as a free weekly community paper