Efficiency of Rz-1 based rhizomania resistance and molecular studies on BNYVV isolates from sugar beet cultivation in Geece

A survey was carried out to investigate the current situation concerning rhizomania disease incidence in sugar beet cultivation of Greece. A systematic field evaluation over locations and years revealed a consistent disease severity pattern according to favourable agroclimatic conditions and pointed to the so far effectiveness of the Rz1 gene-based resistance, as no major disease outbreaks were observed. Molecular analyses aiming at the characterization of the type and genetic diversity of the virus further confirmed the widespread occurrence of BNYVV in the country, as evidenced by RT-PCR amplification of all five known genomic molecules and nested-PCR assays. None of the isolates contained an RNA 5, typically found in pathotype P. On the basis of RFLP patterns, all BNYVV isolates analysed were classified as pathotype A. Sequence determination of the full-length RNA 3-encoded p25 protein, responsible for symptom development, revealed amino acid motifs ACHG/VCHG in the hypervariable region aa67–70. The presence of valine in position 67 did not appear associated with increased pathogenicity and resistance breaking properties, as earlier reported

Detection of beet soil-borne virus and beet virus Q in sugarbeet in Greece

Sugar beet plants with typical rhizomania symptoms were collected from the five major cultivation zones of Greece. The presence of Beet necrotic yellow vein virus (BNYVV), the primary causal agent of the disease, was ascertained by DAS-ELISA in 38 out of 40 fields surveyed and the positive samples were subsequently examined for the presence of other soil-borne viruses which are frequently associated with rhizomania, using a multiplex RT-PCR assay targeting BNYVV, Beet soilborne virus (BSBV) and Beet virus Q (BVQ). The occurrence of BSBV and BVQ was confirmed in 9 and 23 rhizomania-infected fields, respectively. In contrast to surveys conducted in other countries, the presence of BVQ prevailed throughout Greece in dual infections with BNYVV, whereas BSBV was restricted to rhizomania-infected fields from only two sugarbeet cultivation areas. Nine of the samples tested were infected with all three viruses. BSBV was always found in triple infections. To our knowledge, this is the first report of BSBV and BVQ in Greece. The future assessment of the impact of each of these viruses on sugarbeet could prove significant for bleeding objectives in terms of achieving a more durable resistance to the rhizomania syndrome