Evaluation of bio-agent formulations to control Fusarium wilt of tomato

The ED50 value of antagonistic substance from Chaetomium globosum N0802 extracted with hexane was 157 μg/ml. This gave the highest inhibition of conidial production of Fusarium oxysporum f. sp. lycopersici causing tomato wilt var Sida. Crude hexane from Chaetomium lucknowense CLT and crude methanol from Trichoderma harzianum PC01 gave ED50 values of 188 and 192 μg/ml. It clearly demonstrated that antagonistic substances from all tested fungi could be deformed and this could break the conidial cells. The bio-agent formulations namely N0802, CLT and PC01 gave significantly highest disease reduction of tomato wilt which were 44.68, 36.28 and 41.01%, respectively, followed by prochoraz (21.95%). All tested bio-agent formulations could significantly increase the yield of tomato when compared to prochoraz and inoculated control. It is concluded that C. globosum, C. lucknowense and T. harzianumKeywords: Fusarium oxysporum f. sp. lycopersici, Chaetomium globosum, Chaetomium lucknowense, Trichoderma harzianum, bio-agent formulationsAfrican Journal of Biotechnology Vol. 9(36), pp. 5836-5844, 6 September, 201

Copy number alterations and allelic ratio in relation to recurrence of rectal cancer

BACKGROUND: In rectal cancer, total mesorectal excision surgery combined with preoperative (chemo)radiotherapy reduces local recurrence rates but does not improve overall patient survival, a result that may be due to the harmful side effects and/or co-morbidity of preoperative treatment. New biomarkers are needed to facilitate identification of rectal cancer patients at high risk for local recurrent disease. This would allow for preoperative (chemo)radiotherapy to be restricted to high-risk patients, thereby reducing overtreatment and allowing personalized treatment protocols. We analyzed genome-wide DNA copy number (CN) and allelic alterations in 112 tumors from preoperatively untreated rectal cancer patients. Sixty-six patients with local and/or distant recurrent disease were compared to matched controls without recurrence. Results were validated in a second cohort of tumors from 95 matched rectal cancer patients. Additionally, we performed a meta-analysis that included 42 studies reporting on CN alterations in colorectal cancer and compared results to our own data. RESULTS: The genomic profiles in our study were comparable to other rectal cancer studies. Results of the meta-analysis supported the hypothesis that colon cancer and rectal cancer may be distinct disease entities. In our discovery patient study cohort, allelic retention of chromosome 7 was significantly associated with local recurrent disease. Data from the validation cohort were supportive, albeit not statistically significant, of this finding. CONCLUSIONS: We showed that retention of heterozygosity on chromosome 7 may be associated with local recurrence in rectal cancer. Further research is warranted to elucidate the mechanisms and effect of retention of chromosome 7 on the development of local recurrent disease in rectal cancer. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12864-015-1550-0) contains supplementary material, which is available to authorized users

Morphological and molecular phylogeny studies on Eurotiales isolated from soil

Abstract Seven isolates belonging to Eurotiales were isolated from forest soils in the North of Thailand. These isolates were identified and confirmed down to species level by morphological and molecular phylogeny. Six isolates namely: EU02, EU03, EU04, EU07, EU12 and EU14 were identified as Penicillium verruculosum and isolate EU06 was identified as Neosartorya hiratsukae