Aplicação intravesical de amicacina e solução ozonizada em animal com cistite fibrinonecrótica transmural severa

Cystitis is the most common disease of the lower urinary tract. The diagnosis is based on clinical signs and laboratory tests of urine. Due to the indiscriminate use of antibiotics destinated to the treatment, the resistance index has increased, new forms of treatment have been sought, such as ozone therapy and the use of topical antibiotics. The aim of this report is to describe a case of fibrinonecrotic cystitis in a dog treated with ozonized serum and solution of amikacin applied on the intravesical lumen. This treatment proved to be highly effective, since the case was resolved successfully and without recurrence.Pesquisa sem auxílio de agências de fomentoA cistite é a doença mais comum do trato urinário inferior. Seu diagnóstico baseia-se nos sinais clínicos e exames laboratoriais de urina. Devido ao uso indiscriminado de antibióticos para o seu tratamento, o índice de resistência tem aumentado, foram buscadas novos formas de tratamento como ozonioterapia e uso de antibióticos tópicos. Este relato de caso tem como objetivo descrever um caso de cistite fibrinonecrótica em um cão tratado com soro ozonizado e amicacina aplicados de forma tópica intravesical. Este tratamento revelou grande eficácia, já que o caso foi resolvido com sucesso e sem apresentar recidivas.2022-02-1

AAV-Mediated Expression of miR-17 Enhances Neurite and Axon Regeneration In Vitro

Neurodegenerative disorders, including traumatic injuries to the central nervous system (CNS) and neurodegenerative diseases, are characterized by early axonal damage, which does not regenerate in the adult mammalian CNS, leading to permanent neurological deficits. One of the primary causes of the loss of regenerative ability is thought to be a developmental decline in neurons’ intrinsic capability for axon growth. Different molecules are involved in the developmental loss of the ability for axon regeneration, including many transcription factors. However, the function of microRNAs (miRNAs), which are also modulators of gene expression, in axon re-growth is still unclear. Among the various miRNAs recently identified with roles in the CNS, miR-17, which is highly expressed during early development, emerges as a promising target to promote axon regeneration. Here, we used adeno-associated viral (AAV) vectors to overexpress miR-17 (AAV.miR-17) in primary cortical neurons and evaluate its effects on neurite and axon regeneration in vitro. Although AAV.miR-17 had no significant effect on neurite outgrowth and arborization, it significantly enhances neurite regeneration after scratch lesion and axon regeneration after axotomy of neurons cultured in microfluidic chambers. Target prediction and functional annotation analyses suggest that miR-17 regulates gene expression associated with autophagy and cell metabolism. Our findings suggest that miR-17 promotes regenerative response and thus could mitigate neurodegenerative effects

Exames anatomopatológicos em animais selvagens

The necropsy and histopathology in non domestic animals, from wild or captivity, is an important material for various purposes, such as study of anatomy, biology, food habits, parasitism, their diseases, etiologic agent, etc. From the carcasses of these animals can be mounted skeletons and skins (taxidermy) for use in museums, schools, environmental education, among others. From the study of etiologic agents and existing vectors, studies can be done to verify the participation of these animals in the epidemiology as reservoir or as host, of many diseases that affect both, captive or free wild animals, as well domestic animals and humans. Finally this study aims to contribute to "One Health", considering the health of the environmental, animal and human. In addition, the results of the necropsy and histopathology exam sent to the owners provide information for the treatment, prevention, control of the detected diseases.A utilização de exames necroscópicos e histopatológicos em animais selvagens (de vida livre ou de cativeiro) é uma importante ferramenta para diversos fins, tais como: estudo da anatomia, biologia, hábitos alimentares, parasitismo, suas doenças, agente etiológicos. A partir das carcaças desses animais é possível montar os esqueletos e as peles (taxidermia) para utilização em museus, escolas, na educação ambiental, entre outros. Ainda a partir do estudo dos agentes etiológicos e vetores existentes, podem ser feitos estudos para verificar a participação deste animal na epidemiologia de inúmeras doenças que acometem tanto animais selvagens (vida livre ou de cativeiro), assim como animais domésticos (de produção ou de estimação) e os seres humanos. Finalmente este estudo visa o princípio do "One Health", uma só saúde, considerando a saúde ambiental, animal e humana. Além disso, os laudos emitidos referentes aos exames, enviados aos proprietários servem de orientação e esclarecimento para o tratamento, prevenção, controle das enfermidades detectadas

Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora