HPLC/MS-TOF Analysis of Surface Resins from Three Poplar Clones Grown in Serbia

Background and Purpose: Poplar clones grown in Serbia are fast growing tree species important for many different purposes in forestry and industry. In this study chemical content of the surface resins of three poplar clones grown in Serbia -M1, B229 and PE 19/66 was analyzed, aiming at their potential usage as a source of natural products important for pharmacy and chemotaxonomy. Materials and Methods: Using HPLC/MS-TOF we gained the first information on chemical compounds which comprise of resins on terminal twigs cuttings of commonly grown poplar clones. Provided from the nursery of the Institute of Lowland Forestry and Environment (Serbia), terminal twigs cuttings with leaves of different development stage from two year old seedlings of M1 poplar clone (Populus euramericana L.), PE 19/66 clone and B229 clone (both belonging to Populus deltoides) were sampled. The washing of the surface resins from terminal twigs cuttings of every sample was done with methylene-chloride until the samples were prepared for HPLC/MS-TOF analysis. Results: Out of 38 different compounds which were identified, M1 clone qualitatively differed for 14 compounds as compared to two other clones. Generally, the results showed that the composition of the resins consisted of different phenolic acids, phenolic esters, flavonoids and other contents. Conclusion: These three poplar clones are potent producers of pharmacologically and chemotaxonomically potent compounds in forest ecosystems, especially M1 clone

VARIATION IN LEAF PHOTOSYNTHETIC TRAITS OF WILD CHERRY (Prunus avium L.) FAMILIES IN A NURSERY TRIAL

The paper presents the results of investigation of variability of net photosynthesis (A), transpiration (E), stomatal conductance (gs) and water use efficiency (WUE) in one year old seedlings of wild cherry (Prunus avium L.), assessed in a nursery trial. Study involved 5 families of half-sib progenies which originate from the Northern part of Serbia (Vojvodina Province). The results showed significant differences among families in regards of stomatal conductance (0.163-0.256 mol m(-2) s(-1)), transpiration (3.27-5.28 mmol m(-2) s(-1)) and water use efficiency (1.98-3.80 Firnol mmol(-1)) (p <= 3.001), while differences regarding net photosynthesis (10.49-12.44 mu mol m-2 s(-1)) were not statistically significant (p <= 0.124). Canonical discriminant analysis (CDA) was performed in order to estimate multivariate relations among analyzed leaf photosyntetical traits. Families were separated by the first canonical axis (CD1), which described 82\% of variability. Presence of differences in regards of E, gs and WUE indicate the possibility of choosing the best families for a breeding program.Ministry of Education and Science of the Republic of Serbia; project ``Studying climate change and its influence on the environment: impacts, adaptation and mitigation{''} {[}43007

VARIATION IN LEAF PHOTOSYNTHETIC TRAITS OF WILD CHERRY (Prunus avium L.) FAMILIES IN A NURSERY TRIAL

The paper presents the results of investigation of variability of net photosynthesis (A), transpiration (E), stomatal conductance (gs) and water use efficiency (WUE) in one year old seedlings of wild cherry (Prunus avium L.), assessed in a nursery trial. Study involved 5 families of half-sib progenies which originate from the Northern part of Serbia (Vojvodina Province). The results showed significant differences among families in regards of stomatal conductance (0.163-0.256 mol m(-2) s(-1)), transpiration (3.27-5.28 mmol m(-2) s(-1)) and water use efficiency (1.98-3.80 Firnol mmol(-1)) (p <= 3.001), while differences regarding net photosynthesis (10.49-12.44 mu mol m-2 s(-1)) were not statistically significant (p <= 0.124). Canonical discriminant analysis (CDA) was performed in order to estimate multivariate relations among analyzed leaf photosyntetical traits. Families were separated by the first canonical axis (CD1), which described 82\% of variability. Presence of differences in regards of E, gs and WUE indicate the possibility of choosing the best families for a breeding program.Ministry of Education and Science of the Republic of Serbia; project ``Studying climate change and its influence on the environment: impacts, adaptation and mitigation{''} {[}43007