At the Start of the Sarcomere: A Previously Unrecognized Role for Myosin Chaperones and Associated Proteins during Early Myofibrillogenesis

The development of striated muscle in vertebrates requires the assembly of contractile myofibrils, consisting of highly ordered bundles of protein filaments. Myofibril formation occurs by the stepwise addition of complex proteins, a process that is mediated by a variety of molecular chaperones and quality control factors. Most notably, myosin of the thick filament requires specialized chaperone activity during late myofibrillogenesis, including that of Hsp90 and its cofactor, Unc45b. Unc45b has been proposed to act exclusively as an adaptor molecule, stabilizing interactions between Hsp90 and myosin; however, recent discoveries in zebrafish and C. elegans suggest the possibility of an earlier role for Unc45b during myofibrillogenesis. This role may involve functional control of nonmuscle myosins during the earliest stages of myogenesis, when premyofibril scaffolds are first formed from dynamic cytoskeletal actin. This paper will outline several lines of evidence that converge to build a model for Unc45b activity during early myofibrillogenesis

Path length distribution in two-dimensional causal sets

We study the distribution of maximal-chain lengths between two elements of a causal set and its relationship with the embeddability of the causal set in a region of flat spacetime. We start with causal sets obtained from uniformly distributed points in Minkowski space. After some general considerations we focus on the 2-dimensional case and derive a recursion relation for the expected number of maximal chains $n_k$ as a function of their length $k$ and the total number of points $N$ between the maximal and minimal elements. By studying these theoretical distributions as well as ones generated from simulated sprinklings in Minkowski space we identify two features, the most probable path length or peak of the distribution $k_0$ and its width $\Delta$, which can be used both to provide a measure of the embeddability of the causal set as a uniform distribution of points in Minkowski space and to determine its dimensionality, if the causal set is manifoldlike in that sense. We end with a few simple examples of $n_k$ distributions for non-manifoldlike causal sets.Comment: 9 pages, 5 figure

Thurston equivalence of topological polynomials

We answer Hubbard's question on determining the Thurston equivalence class of ``twisted rabbits'', i.e. images of the ``rabbit'' polynomial under n-th powers of the Dehn twists about its ears. The answer is expressed in terms of the 4-adic expansion of n. We also answer the equivalent question for the other two families of degree-2 topological polynomials with three post-critical points. In the process, we rephrase the questions in group-theoretical language, in terms of wreath recursions.Comment: 40 pages, lots of figure

Natural history of irritable bowel syndrome

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73897/1/j.1365-2036.2004.01929.x.pd

Dynamical Belyi maps

We study the dynamical properties of a large class of rational maps with exactly three ramification points. By constructing families of such maps, we obtain infinitely many conservative maps of degree $d$; this answers a question of Silverman. Rather precise results on the reduction of these maps yield strong information on the rational dynamics.Comment: 21 page

Life history parameters in acellular extrinsic fiber cementum microstructure

Life-history parameters such as pregnancies, skeletal trauma, and renal disease have previously been identified from hypomineralized growth layers (incremental lines) of acellular extrinsic fiber cementum (AEFC). The precise periodicity of these growth layers remains vaguely approximated, so causal life-history explanations using tooth cementum cannot yet be rigorously calculated or tested. On the other hand, we show how life history parameters in AEFC can be identified by two contrasting elemental detection methods. Based on our results we reject the possibility of accurate estimation of pregnancies and other life history parameters from cementum using scanning electron microscopy alone. Here, we propose a new methodological approach for cementum research, Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS), to measure degree and distribution of mineralization of cementum growth layers. Our results show that Tof-SIMS can significantly increase our knowledge of cementum composition and is therefore a powerful new tool for life history researchers

Assessing temporal genetic variation in a cougar population: influence of harvest and neighboring populations

The geography of the Black Hills region of South Dakota and Wyoming may limit connectivity for many species. For species with large energetic demands and large home ranges or species at low densities this can create viability concerns. Carnivores in this region, such as cougars (Puma concolor), have the additive effect of natural and human-induced mortality; this may act to decrease long-term viability. In this study we set out to explore genetic diversity among cougar populations in the Black Hills and surrounding areas. Specifically, our objectives were to first compare genetic variation and effective number of breeders of cougars in the Black Hills during three harvest regimes: pre (2003–2006), moderate (2007–2010), and heavy (2011–2013), to determine if harvest impacted genetic variation. Second, we compared genetic structure of the Black Hills cougar population with cougar populations in neighboring eastern Wyoming and North Dakota. Using 20 microsatellite loci, we conducted genetic analysis on DNA samples from cougars in the Black Hills (n = 675), North Dakota (n = 113), and eastern Wyoming (n = 62) collected from 2001–2013. Here we report that the Black Hills cougar population maintained genetic variation over the three time periods. Our substructure analysis suggests that the maintenance of genetic variation was due to immigration from eastern Wyoming and possibly North Dakota