Simplification of real-estate contracts with the Land Administration System: a case study of Poland

This article examines the practicalities of Notaries Public using the Land Administration System for e-service transfer of property ownership. Herein, we use the example of the Polish Integrated Real Estate Information System (IREIS) in the activities of a Notary Public. Good governance and its genesis and IREIS developmental stages are initially assessed with emphasis on the functionality of the system and integration of the databases containing the information needed to prepare for notarial duties. The most important legal provisions applied to the conducting of professional duties by notaries are then discussed. These notably include the provisions of Notary Law and the Civil Code. Notarial tasks were then examined with regard to the possibility and admissibility of using the IREIS to accelerate their activities, decrease delays in essential formalities and minimize associated costs. Conclusions are drawn, and these include the forecast of future innovative solutions by e-service communication

Usefulness of RAPD, RFLP and SCAR molecular markers and AGPaseB gene methylation level in the screening of resistance to the golden cyst nematode (Globodera rostochiensis) pathotype Ro1 in different Polish potato genotypes

Abstract The aim of this study was to assess the application of various DNA markers as well as the ADP-glucose pyrophosphorylase small subunit (AGPaseB) gene methylation for the screening of potato cultivars and breeding lines with different resistance to Globodera rostochiensis. Tetraploid genotypes were included. The 2 kb and 0.7 kb random amplified polymorphic (RAPD) fragments as well as a PCR-amplified 1.6 kb fragment of AGPaseB gene positioned close to the Gro1 locus were tested. Two novel sequence characterised amplified region (SCAR) fragments were generated from 2 kb RAPD product of susceptible and resistant genotypes. Significant correlation (r=0.32) was detected only between the presence of SCARI and SCARII fragments markers in all the investigated genotypes. Three different allelic forms of the AGPaseB gene were detected in tetraploid potato genotypes. One of these allelic forms may be closely linked with Gro1. A strong methylation occurred at promoter region and the 5' part of AGPaseB gene which was generally absent at its 3' UTR region. The methylation pattern of AGPaseB investigated by restriction with MspI and HpaII varied between some G. rostochiensis resistant and sensitive genotypes. We concluded that methylation profiling of AGPaseB gene should be promising for the development of practical assays for resistance to nematodes in potato

NGS analysis of collagen type I genes in Polish patients with Osteogenesis imperfecta: a nationwide multicenter study

Osteogenesis imperfecta (OI) is a rare genetic disorder of the connective tissue. It presents with a wide spectrum of skeletal and extraskeletal features, and ranges in severity from mild to perinatal lethal. The disease is characterized by a heterogeneous genetic background, where approximately 85%–90% of cases have dominantly inherited heterozygous pathogenic variants located in the COL1A1 and COL1A2 genes. This paper presents the results of the first nationwide study, performed on a large cohort of 197 Polish OI patients. Variants were identified using a next-generation sequencing (NGS) custom gene panel and multiplex ligation probe amplification (MLPA) assay. The following OI types were observed: 1 (42%), 2 (3%), 3 (35%), and 4 (20%). Collagen type I pathogenic variants were reported in 108 families. Alterations were observed in α1 and α2 in 70% and 30% of cases, respectively. The presented paper reports 97 distinct causative variants and expands the OI database with 38 novel pathogenic changes. It also enabled the identification of the first glycine-to-tryptophan substitution in the COL1A1 gene and brought new insights into the clinical severity associated with variants localized in “lethal regions”. Our results contribute to a better understanding of the clinical and genetic aspects of OI

Characterization of patients with pulmonary arterial hypertension : data from the polish registry of pulmonary hypertension (BNP-PL)

Current knowledge of pulmonary arterial hypertension (PAH) epidemiology is based mainly on data from Western populations, and therefore we aimed to characterize a large group of Caucasian PAH adults of Central-Eastern European origin. We analyzed data of incident and prevalent PAH adults enrolled in a prospective national registry involving all Polish PAH centers. The estimated prevalence and annual incidence of PAH were 30.8/mln adults and 5.2/mln adults, respectively and they were the highest in females ≥65 years old. The most frequent type of PAH was idiopathic (n = 444; 46%) followed by PAH associated with congenital heart diseases (CHD-PAH, n = 356; 36.7%), and PAH associated with connective tissue disease (CTD-PAH, n = 132; 13.6%). At enrollment, most incident cases (71.9%) were at intermediate mortality risk and the prevalent cases had most of their risk factors in the intermediate or high risk range. The use of triple combination therapy was rare (4.7%). A high prevalence of PAH among older population confirms the changing demographics of PAH found in the Western countries. In contrast, we found: a female predominance across all age groups, a high proportion of patients with CHD-PAH as compared to patients with CTD-PAH and a low use of triple combination therapy

Die Schifffahrtspolitik und die Markierung des Schifffahrtsweges Stettin–Swinemünde in den Jahren 1820–1945

W artykule ukazano ważność dróg wodnych prowadzących do Szczecina – ważnego portu przeładunkowego towarów transportowanych drogami śródlądowymi i morskimi. Rozwój handlu i spory ruch żeglugowy na trasie Bałtyk–Szczecin wymusiły na władzach modernizację portu morskiego w Świnoujściu, podejścia do tego portu oraz dróg wodnych prowadzących do Szczecina. Modernizacja portu i dróg wodnych przebiegała etapami i była związana z rozwojem różnorodnych znaków nawigacyjnych, które zapewniały odpowiedni poziom bezpieczeństwa żeglugi. Z drugiej strony polityka żeglugowa zmierzała do dopuszczenia do żeglugi na trasie Świnoujście–Szczecin statków o większym tonażu, a tym samym o większym zanurzeniu. Ten proces wymuszał konieczność okresowego pogłębiania głównego toru wodnego. W rezultacie tych i innych działań kompleks portowy Świnoujście-Szczecin stał się jednym z ważniejszych węzłów tego typu na południowym Bałtyku. Konkurował on z węzłami Piława-Królewiec i kompleksem portowym Gdańska

Resources and adaptation following involuntary resettlement in the Bytom-Karb community

Studies show that involuntary displacement often creates various threats for the community and individuals. To reduce these risks, Environmental and Social Impact Assessment, Health Impact Assessment, and Social Assessment are recommended. Whereas assessments focus mostly on the community level and studies describe cases of large population displacements, there is a lack of empirical evidence about how individuals cope with involuntary displacement and what factors contribute or hinder their successful adaptation in the target location. This study uses semi-structured interviews with 21 people about their experience of resettlement due to a mine collapse in Bytom, Poland, that led to involuntary displacement of 560 people. Data was analyzed according to the constructivist grounded theory principles. Results show that this case illustrates a mixture of post-disaster and development-induced displacement. Various factors and resources that affected coping strategies were analyzed, including: material and legal status, health and age, communication skills, and relocation experience. Our findings suggest that, when circumstances allow, an individual resources assessment should also be conducted to counteract impoverishment and further marginalization of the disprivileged and vulnerable individuals

Association of Novel Advanced Glycation End-Product (AGE10) with Complications of Diabetes as Measured by Enzyme-Linked Immunosorbent Assay

Advanced glycation end-products (AGEs) contribute to vascular complications and organ damage in diabetes. The unique AGE epitope (AGE10) has recently been identified in human serum using synthetic melibiose-derived AGE (MAGE). We aimed at developing ELISA for AGE10 quantification, determining whether AGE10 is present in diabetic patients (n = 82), and evaluating its association with diabetic complications. In a competitive ELISA developed, the reaction of synthetic MAGE with anti-MAGE was inhibited by physiological AGE10 present in serum. In this assay, new murine IgE anti-MAGE monoclonal antibodies, which do not recognize conventional AGEs, a synthetic MAGE used to coat the plate, and LMW-MAGE (low molecular mass MAGE) necessary to plot a standard curve were used. AGE10 was significantly higher in patients with microangiopathy, in whom it depended on treatment, being lower in patients treated with aspirin. AGE10 levels were positively correlated with estimated glomerular filtration rate (eGFR) and negatively with creatinine. As a marker of stage ≥3 chronic kidney disease or microangiopathy, AGE10 displayed moderate overall accuracy (respectively, 69% and 71%) and good sensitivity (82.6% and 83.3%) but poor specificity (58.1% and 57.8%). In conclusion, newly developed immunoassay allows for AGE10 quantification. AGE10 elevation is associated with microangiopathy while its decrease accompanies stage ≥3 chronic kidney disease