Recent insight into the role of macrophage in alcohol-associated liver disease: a mini-review

Alcohol-associated liver disease (ALD) is a condition that develops due to prolonged and excessive alcohol consumption. It encompasses various stages of liver damage, including fatty liver, alcoholic hepatitis, and cirrhosis. Immune cells, particularly macrophages, of various types play a significant role in the onset and progression of the disease. Macrophages observed in the liver exhibit diverse differentiation forms, and perform a range of functions. Beyond M1 and M2 macrophages, human macrophages can polarize into distinct phenotypes in response to various stimuli. Recent advancements have improved our understanding of macrophage diversity and their role in the progression of ALD. This mini-review provides a concise overview of the latest findings on the role and differentiation of macrophages in ALD. Additionally, it discusses potential therapeutic targets associated with macrophages and explores potential therapeutic strategies

Integer estimation methods for GPS ambiguity resolution: an applications oriented review and improvement

The integer least squares (ILS) problem, also known as the weighted closest point problem, is highly interdisciplinary, but no algorithm can find its global optimal integer solution in polynomial time. We first outline two suboptimal integer solutions, which can be important either in real time communication systems or to solve high dimensional GPS integer ambiguity unknowns. We then focus on the most efficient algorithm to search for the exact integer solution, which is shown to be faster than LAMBDA in the sense that the ratio of integer candidates to be checked by the efficient algorithm to those by LAMBDA can be theoretically expressed by rm, where r[m] and m is the number of integer unknowns. Finally, we further improve the searching efficiency of the most powerful combined algorithm by implementing two sorting strategies, which can either be used for finding the exact integer solution or for constructing a suboptimal integer solution. Test examples clearly demonstrate that the improved methods can perform significantly better than the most powerful combined algorithm to simultaneously find the optimal and second optimal integer solutions, if the ILS problem cannot be well reduced

New morphological and DNA evidence supports the existence of Calligonum jeminaicum Z. M. Mao (Calligoneae, Polygonaceae) in China

Calligonum jeminaicum Z. M. Mao, a species regarded as endemic to China, was thought to be nonexistent owing to a lack of scientific records. The similarity of C. jeminaicum to C. mongolicum Turcz. warranted an investigation into the taxonomical relationship between these species. In this study, a naturally occurring population of C. jeminaicum was discovered and the taxonomical relationships of this species with C. mongolicum were resolved. Morphological traits, including fruit and flower characteristics, as well as nuclear (ETS, ITS) and chloroplast (psbA-trnH, ycf6-psbM, rpl32-trnL, rbcL, and trnL-F) DNA sequence data were studied to confirm the taxonomic status of C. jeminaicum. The nrDNA data (ITS1-2 and ETS) from C. jeminaicum reflected variability from the whole C. mongolicum complex, showing distinctive haplotypes in the Calligonum sect. Medusa Sosk. & Alexandr. The cpDNA data supplied similar evidence, showing unique branching in Bayesian and ML tree analyses. The specific status of C. jeminaicum is confirmed based on both morphological and molecular analyses. Here we present a revised description of C. jeminaicum along with its DNA barcode and discuss suggestions for the conservation of this species. Based on current evidence, this species was evaluated as Critically Endangered (CR) according to the IUCN criteria

Transcriptome profiling analysis of Mactra veneriformis by deep sequencing after exposure to 2,2',4,4'-tetrabromodiphenyl ether

Polybrominated diphenyl ethers (PBDEs) are ubiquitous global pollutants, which are known to have immune, development, reproduction, and endocrine toxicity in aquatic organisms, including bivalves. 2,2',4,4'-Tetrabromodiphenyl ether (BDE-47) is the predominant PBDE congener detected in environmental samples and the tissues of organisms. However, the mechanism of its toxicity remains unclear. In this study, high-throughput sequencing was performed using the clam Mactra veneriformis, a good model for toxicological research, to clarify the transcriptomic response to BDE-47 and the mechanism responsible for the toxicity of BDE-47. The clams were exposed to 5 mu g/L BDE-47 for 3 days and the digestive glands were sampled for high-throughput sequencing analysis. We obtained 127 648, 154 225, and 124 985 unigenes by de novo assembly of the control group reads (CG), BDE-47 group reads (BDEG), and control and BDE-47 reads (CG & BDEG), respectively. We annotated 32 176 unigenes from the CG & BDEG reads using the NR database. We categorized 24 401 unigenes into 25 functional COG clusters and 21 749 unigenes were assigned to 259 KEGG pathways. Moreover, 17 625 differentially expressed genes (DEGs) were detected, with 10 028 upregulated DEGs and 7 597 downregulated DEGs. Functional enrichment analysis showed that the DEGs were involved with detoxification, antioxidant defense, immune response, apoptosis, and other functions. The mRNA expression levels of 26 DEGs were verified by quantitative real-time PCR, which demonstrated the high agreement between the two methods. These results provide a good basis for future research using the M. veneriformis model into the mechanism of PBDEs toxicity and molecular biomarkers for BDE-47 pollution. The regulation and interaction of the DEGs would be studied in the future for clarifying the mechanism of PBDEs toxicity

A large scale of apparent sudden movements in Japan detected by high-rate GPS after the 2011 Tohoku Mw9.0 earthquake: Physical signals or unidentified artifacts?

Abstract A moment magnitude Mw9.0 earthquake hit northeastern Japan at 14:46:18 (Japan Standard Time), March 11, 2011. We have obtained 1 s precise point positioning solutions for 1198 GEONET stations. Although GPS position time series have been routinely investigated and used as waveforms for dynamic inversion of earthquakes, we focus on exploring the spatial displacement features of GEONET stations for this earthquake. A movie inspection of high-rate GPS waveforms leads us to find that 76.21% of the GEONET stations in the Japanese islands subsided suddenly within 1 s between 14:59:45 and 14:59:46, Japan local time, with an average displacement of $$-\,2.43$$ -2.43 , 2.83 and $$-\,4.75$$ -4.75  mm in the east, north and vertical components, respectively, about 15 min after the 2011 Tohoku earthquake. We have performed different types of independent tests, namely measurement error analysis, processing the GEONET data with a different software system, a statistical hypothesis testing under a simple assumption of sign distributions, the test computation of the displacement field outside of the Japanese islands and an independent test with the Japanese strong motion borehole network KiK-net, to see whether these sudden movements actually occurred. The first four independent tests are passed almost without any doubt, and the direction of the average sudden displacements is roughly consistent tectonically with the direction of subduction of the Pacific plate. Because there are only 78 KiK-net borehole stations available for an independent seismic test, the KiK-net results are marginally consistent with those of GEONET. In the daily seismological and geophysical practice, one may then conclude that the sudden movement within the second is real after passing these five independent tests. However, a further epoch-by-epoch check pinpoints a few more seconds with even a higher probability of sudden displacement from the 20-min three-component high-rate GPS waveforms after the main shock, or more precisely, the seconds between 14:59:04 and 14:59:05, 15:01:04 and 15:01:05, and 15:03:39 and 15:03:40 with 80.80, 84.14 and 85.89% of the GEONET stations simultaneously moving upward, southward and westward, respectively. Although these probabilities are very high, it may hardly be imagined that a large scale of sudden movements could occur repeatedly between 14:59:04 and 15:03:40. The high-rate GPS results imply that some detected sudden movements after the earthquake could be unidentified artifacts of GPS data processing, though we cannot rule out the possibility that the detected sudden movements in Japan after the 2011 Tohoku Mw9.0 earthquake are real physical signals

Genetic Association between Methylenetetrahydrofolate Reductase Gene Polymorphism and Risk of Osteonecrosis of the Femoral Head

Background. Methylenetetrahydrofolate reductase (MTHFR) SNP rs1801133 has been frequently investigated in recent years. Relevant candidate gene association studies with this SNP and osteonecrosis of the femoral head (ONFH) reported conflicting results. Meta-analysis provides a method to combine these data and to determine the association in a larger sample size. Method. We conducted a systematic search to identify possible studies. Four pooled ORs (odds ratios, T versus C, TT versus CC, TT/CT versus CC, and TT versus CT/CC), along with 95% confidence interval (CI), were calculated to evaluate the association between SNP rs1801133 and ONFH susceptibility. Both fixed effects model and random effects model were used. Findings. We eventually included twelve studies in this analysis, with results showing no overall association between ONFH susceptibility and SNP rs1801133 (T versus C: OR=1.15, 95% CI=0.97–1.38; TT versus CC: OR=1.15, 95% CI=0.91–1.46; TT/CT versus CC: OR=1.09, 95% CI=0.95–1.25; and TT versus CT/CC: OR=1.16, 95% CI=0.93–1.45). When stratified based on ethnicity, the results were still not significant. Conclusion. Our findings are generally supportive of no association between MTHFR SNP rs1801133 and the etiology of ONFH