Rudimentary G-Quadruplex-Based Telomere Capping In Saccharomyces Cerevisiae

Telomere capping conceals chromosome ends from exonucleases and checkpoints, but the full range of capping mechanisms is not well defined. Telomeres have the potential to form G-quadruplex (G4) DNA, although evidence for telomere G4 DNA function in vivo is limited. In budding yeast, capping requires the Cdc13 protein and is lost at nonpermissive temperatures in cdc13-1 mutants. Here, we use several independent G4 DNA-stabilizing treatments to suppress cdc13-1 capping defects. These include overexpression of three different G4 DNA binding proteins, loss of the G4 DNA unwinding helicase Sgs1, or treatment with small molecule G4 DNA ligands. In vitro, we show that protein-bound G4 DNA at a 3\u27 overhang inhibits 5\u27-\u3e 3\u27 resection of a paired strand by exonuclease I. These findings demonstrate that, at least in the absence of full natural capping, G4 DNA can play a positive role at telomeres in vivo

The Effect of Childhood Sexual Victimization on Women's Income

Numerous studies show that survivors of childhood sexual abuse (CSA) suffer as adults from depression, post-traumatic stress disorder, alcohol and drug abuse, and other mental illnesses. As such, the effect of experiencing traumatic events during childhood including sexual abuse can be long lasting. The lasting effects of CSA may also have economic implications. The purpose of this paper is to examine whether a history of CSA affects women's wages and to examine whether such effects are a function of the severity of abuse. Eastern Economic Journal (2008) 34, 27–40. doi:10.1057/palgrave.eej.9050017