TNFα protein secreted by MDM cells exposed to over time was measured by ELISA

<p><b>Copyright information:</b></p><p>Taken from "Cytokine responses of bovine macrophages to diverse clinical subspecies strains"</p><p>BMC Microbiology 2006;6():10-10.</p><p>Published online 14 Feb 2006</p><p>PMCID:PMC1382238.</p><p>Copyright © 2006 Janagama et al; licensee BioMed Central Ltd.</p> Total amount of protein (pg/ml) is plotted on Y-axis (note that the Y1-axis scales have been optimized for magnitude of TNFα expression for each strain). Intracellular bacterial numbers were calculated based on the genome size of and represented as genome equivalents (GE) on second Y-axis. MDMs stimulated with B1018 secreted low amounts of TNFα protein relative to other cell stimulations

TNFα mRNA expressed by MDM cells exposed to over time was measured by Real time RT PCR and fold change in gene expression relative to β actin was calculated by 2method

<p><b>Copyright information:</b></p><p>Taken from "Cytokine responses of bovine macrophages to diverse clinical subspecies strains"</p><p>BMC Microbiology 2006;6():10-10.</p><p>Published online 14 Feb 2006</p><p>PMCID:PMC1382238.</p><p>Copyright © 2006 Janagama et al; licensee BioMed Central Ltd.</p> Mean fold change in gene expression is plotted on Y-axis (note that the Y1-axis scales have been optimized for magnitude of IL-10 expression for each strain). Intracellular bacterial numbers based on the amplification of were calculated based on the genome size of and represented as genome equivalents (GE) on second Y-axis. MDMs stimulated with B1018 expressed lower levels of TNFα mRNA relative to other cell stimulations