TNFα mRNA expressed by MDM cells exposed to over time was measured by Real time RT PCR and fold change in gene expression relative to β actin was calculated by 2method

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Cytokine responses of bovine macrophages to diverse clinical subspecies strains"</p><p>BMC Microbiology 2006;6():10-10.</p><p>Published online 14 Feb 2006</p><p>PMCID:PMC1382238.</p><p>Copyright © 2006 Janagama et al; licensee BioMed Central Ltd.</p> Mean fold change in gene expression is plotted on Y-axis (note that the Y1-axis scales have been optimized for magnitude of IL-10 expression for each strain). Intracellular bacterial numbers based on the amplification of were calculated based on the genome size of and represented as genome equivalents (GE) on second Y-axis. MDMs stimulated with B1018 expressed lower levels of TNFα mRNA relative to other cell stimulations