3D Printing of Regenerated Silk Fibroin and Antibody-Containing Microstructures via Multiphoton Lithography

Regenerated silk fibroin, a biopolymer derived from silkworm cocoons, is a versatile material that has been widely explored for a number of applications (e.g., drug delivery, tissue repair, biocompatible electronics substrates, and optics) due to its attractive biochemical properties and processability. Here, we report on the free-form printing of silk-based, 3D microstructures through multiphoton lithography. Utilizing multiphoton lithography in conjunction with specific photoinitiator chemistry and postprint cross-linking, a number of microarchitectures were achieved including self-supporting fibroin arches. Further, the straightforward production of high fidelity and biofunctional protein architectures was enabled through the printing of aqueous fibroin/immunoglobulin solutions

Surface Analysis

Surface analysis of water soluble proteins as a function of surface chemistr

Enzyme-Polymer Simulation

Simulation of protein-random heteropolymer complex in solution

Sequence simulation of random heteropolymer

The file contains simulated sequences of random heteropolymers and block length histogram analysis