28 research outputs found
Enteric Protozoa of Some Amphibians of the Elk Mountains, Colorado
Author Institution: Department of Velerinary Medicine, Oregon State University, Corvallis, Orego
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Histochemical study of some enzymes of the common liver fluke, Fasciola hepatica L.
Acid and alkaline phosphatase was localized histochemically in the intestinal epithelium and excretory duct respectively in fresh
frozen sections of Fasciola hepatica. Polyphenol oxidase was
localized in the vitellaria, vitelline ducts and egg-shells. The distribution
and nature of esterases was examined histochemically. Acetyl-cholinesterase
was observed in the tegument, neural ganglia, neural
commisure, acetabulum and oral sucker. Pseudocholinesterase was
observed in the pharynx, cirrus, cirrus pouch wall, prostate gland
sperm sac, testes, ovary, vitelline duct and duct walls of Mehlis'
gland. Use of differential inhibitors of mammalian cholinesterases
indicated that presence of acetylcholinesterase in structures containing
pseudocholinesterase could not be demonstrated by differential
enzyme activity. Non-specific esterase is reported in the excretory
ducts and intestinal epithelium. The possible functions of the various
esterases is discussed. Succinic dehydrogenase was observed in all
those organs and tissues reported to contain esterases. It is reported
from the cirrus, cirrus pouch wall, prostate gland and sperm
sac for the first time.
The effects of various concentrations of Ruelene (4-tert-butyl-2-chlorophenyl methyl methyl phosphoramidate), Halaxon (di(2-chloroethyl) 3-chloro-4 methylcoumarin-7-yl) phosphate, CoRal
(0, 0-diethyl-0-3-chloro-4-methyl-2-oxo-2H-l-benzyopyran-7-yl phosphorothioate), Dibenzylamine (DABA, 1, 6 bis (methylamino-methylphenoxy)
hexane dihydrochloride), Tris (p-aminophenyl) carbonium
chloride (TAC, pararosaniline chloride), Bayer 9015 (B9015,
3, 3'-dichloro-5, 5'-dinitro-0, 0'-biphenol), Bithionol (2, 2'-thiobis
(4, 6-dichlorophenol) 'Actamer') were examined for inhibitory effect
against fluke cholinesterases. Bithionol and B9015 failed to inhibit
esterases activity. CoRal, Neguvon, TAC and Halaxon inhibited all
cholinesterase activity. Ruelene reversibly inhibited pharyngeal and
reproductive complex cholinesterases but non-reversibly inhibited
tegumental and neural Acetylcholinesterase. Dibenzylamine reversibly
inhibited all cholinesterases.
Bayer 9015 and DABA failed to inhibit excretory duct alkaline
phosphatase activity. Bayer 9015 failed to inhibit acid phosphatase
activity in intestinal epithelium.
Bithionol, Bayer 9015 and Hexachlorophene (2, 2' dihydroxy-3, 5, 6-3'5'6' hexachlorodiphenyl methane) all inhibited
succinic dehydrogenase at all concentrations tested. Dibenzylamine
had no inhibitory effect while TAC partially inhibited the
staining reaction.
Incubation of adult flukes in DABA-Hedon-Fleig saline for 8,
18 or 24 hours resulted in a decrease in PAS positive material and
loss of tegument. This is compared with Bayer 9015 induced necrosis
and a hypothesis explaining the mechanism of this necrosis is
presented
INCIDENCE OF COCCIDIA (EIMERIDAE) IN ADULT EWES AND LAMBS ON ST. CROIX
INCIDENCE OF COCCIDIA (EIMERIDAE) IN ADULT EWES AND LAMBS ON ST. CROI
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Analyses of pig genomes provide insight into porcine demography and evolution
For 10,000 years pigs and humans have shared a close and complex relationship. From domestication to modern breeding practices, humans have shaped the genomes of domestic pigs. Here we present the assembly and analysis of the genome sequence of a female domestic Duroc pig (Sus scrofa) and a comparison with the genomes of wild and domestic pigs from Europe and Asia. Wild pigs emerged in South East Asia and subsequently spread across Eurasia. Our results reveal a deep phylogenetic split between European and Asian wild boars ∼1 million years ago, and a selective sweep analysis indicates selection on genes involved in RNA processing and regulation. Genes associated with immune response and olfaction exhibit fast evolution. Pigs have the largest repertoire of functional olfactory receptor genes, reflecting the importance of smell in this scavenging animal. The pig genome sequence provides an important resource for further improvements of this important livestock species, and our identification of many putative disease-causing variants extends the potential of the pig as a biomedical model
Restructuring the University - An Experiential Exercise
The author presents and comments on an experiential exercise addressing organizational structure and goal attainment
Tinte: developing a prototype for typesetting music in Clean : a case study
This paper describes the development of a typesetting program for music in the lazy functional programming language Clean. The system transforms a description of the music to be typeset in a dvi-file just like TEX does with mathematical formulae. The implementation makes heavy use of higher order functions. It has been implemented in just a few weeks and is able to typeset quite impressive examples. The system is easy to maintain and can be extended to typeset arbitrary complicated musical constructs. The paper can be considered as a status report of the implementation as well as a reference manual for the resulting system
Termination proofs for a lazy functional language by abstract reduction : (draft)
Automatic termination proofs of functional programming languages are an often challenged problem Most work in this area is done on strict languages Orderings for arguments of recursive calls are generated In lazily evaluated languages arguments for functions are not necessarily evaluated to a normal form It is not a trivial task to de ne orderings on expressions that are not in normal form or that do not even have a normal form We propose a method based on an abstract reduction process that reduces up to the point when su cient ordering relations can be found The proposed method is able to nd termination proofs for lazily evaluated programs that involve non terminating subexpressions Analysis is performed on a higher order polymorphic typed language and termi nation of higher order functions can be proved too The calculus can be used to derive information on a wide range on di erent notions of termination